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Team:Bielefeld-CeBiTec/Notebook/Journal/rMFC/Jul
From 2014.igem.org
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<li><b> oprF </b></li> | <li><b> oprF </b></li> | ||
<ul> | <ul> | ||
| - | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of | + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of pSB1C3_<i>oprF</i> </li> |
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification </a> of <i>oprF</i> for integration into chromosome (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#porine_fwd_FRT" target="_blank"> porine_fwd_FRT</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#porine_rev_FRT " target="_blank"> porine_rev_FRT </a>)</li> | ||
| + | <ul> | ||
| + | <li>Annealing temperature: 55 °C</li> | ||
| + | <li>bands are as expected (1,36 kb) </li> | ||
| + | </ul> | ||
| + | <li><b> RedET </b></li> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of RedEt plasmid </li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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Revision as of 09:59, 29 September 2014
 |
July |
 |
- Amplification of pSB1C3 backbone for FumA and FumBCD
- This week we amplified the pSB1C3 backbone of FumA and FumBCD to use it for Gibson Assembly afterwards
- PCR amplification ( pSB1C3_pre_Fum_A, pSB1C3_suf_Fum_A )
- Annealing temperature: 55 °C
- Bands are as expected (2,07 kb)
- PCR amplification ( pSB1C3_pre_Fum_BCD, pSB1C3_suf_Fum_BCD )
- Annealing temperature: 55 °C
- Bands are as expected (2,07 kb)
- PCR purification of backbones
- oprF
- Plasmid isolation of pSB1C3_oprF
- PCR amplification of oprF for integration into chromosome ( porine_fwd_FRT, porine_rev_FRT )
- Annealing temperature: 55 °C
- bands are as expected (1,36 kb)
- RedET
- Plasmid isolation of RedEt plasmid
