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Team:Bielefeld-CeBiTec/Notebook/Journal/rMFC/Jul
From 2014.igem.org
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<li> This week we amplified the pSB1C3 backbone of FumA and FumBCD to use it for Gibson Assembly afterwards </li> | <li> This week we amplified the pSB1C3 backbone of FumA and FumBCD to use it for Gibson Assembly afterwards </li> | ||
<ul> | <ul> | ||
| - | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification</a></li> | + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification </a>(<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_Fum_A" target="_blank"> pSB1C3_pre_Fum_A</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_Fum_A " target="_blank"> pSB1C3_suf_Fum_A </a>)</li> |
| + | <ul> | ||
| + | <li>Annealing temperature: -- °C</li> | ||
| + | <li>Bands are as expected (2,07 kb)</li> | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 13:52, 28 September 2014
 |
July |
 |
- Amplification of pSB1C3 backbone for FumA and FumBCD
- This week we amplified the pSB1C3 backbone of FumA and FumBCD to use it for Gibson Assembly afterwards
- PCR amplification ( pSB1C3_pre_Fum_A, pSB1C3_suf_Fum_A )
- Annealing temperature: -- °C
- Bands are as expected (2,07 kb)
