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Team:Bielefeld-CeBiTec/Notebook/Journal/Isobutanol/Sep
From 2014.igem.org
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| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#RestrictionDigestion" target="_blank">Restriction digestion</a> with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionEnzymes" target="_blank"><i>EcoR</i>I</a> and <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionEnzymes" target="_blank"><i>Pst</i>I</a> of pSB1K3_<i>adhA</i> </li> | ||
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| + | <li>Bands as expected (~1200 bp)</li> | ||
| + | <div class="element" style="height:350px; width:150px; text-align:center"> | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/9a/Bielefeld_CeBiTec_2014-10-14_adhA_Kontrollverdau_09_01.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/9/9a/Bielefeld_CeBiTec_2014-10-14_adhA_Kontrollverdau_09_01.png" height="230px"></a><br><font size="1">Agarose gel from the restriction digestion with <i>EcoR</i>I and <i>Pst</i>I. As a Ladder we used <a href="http://www.thermoscientificbio.com/nucleic-acid-electrophoresis/generuler-1-kb-dna-ladder-ready-to-use-250-to-10000-bp">GeneRuler™ 1 kb DNA Ladder from Thermo Scientific</a>. </font> | ||
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Revision as of 07:57, 15 October 2014
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September |
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- Restriction digestion with EcoRI and PstI of pSB1K3_adhA
- Bands as expected (~1200 bp)
Agarose gel from the restriction digestion with EcoRI and PstI. As a Ladder we used GeneRuler™ 1 kb DNA Ladder from Thermo Scientific.
