Team:Bielefeld-CeBiTec/Notebook/Journal/Isobutanol/Aug

From 2014.igem.org

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          <li>Bands as expected (3,3 kb)</li>
          <li>Bands as expected (3,3 kb)</li>
              </ul>
              </ul>
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                       <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of pSB1C3-alsS-ilvC-ilvD-kivD</li>
+
                       <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of pSB1K3-alsS-ilvC-ilvD-kivD</li>
                       <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#RestrictionDigestion" target="_blank">Restriction digestion</a> with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionEnzymes" target="_blank"><i>NotI</i></a></li>
                       <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#RestrictionDigestion" target="_blank">Restriction digestion</a> with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionEnzymes" target="_blank"><i>NotI</i></a></li>
              <ul>
              <ul>

Revision as of 16:01, 25 September 2014


August

  • psB1C3-alsS-ilvC-ilvD
  • alsS, ilvC, ilvD, kivD and backbone pSB1K3
    • This week we tried to begin from zero again with optimized conditions and new competent cells.
      Therefor we prepared new plasmids from alsS, ilvC, ilvD, kivD and the backbone pSB1K3-RFP