Team:UESTC-China/Protocol

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Revision as of 04:13, 24 September 2014

UESTC-China

DNA ligation with T4 DNA Ligase
Component 20ul Reaction
1.33X Master Mix 15ul
Insert DNA the moles of insert DNA to vector DNA is 5：1
>Vector DNA 50ng
ddH2O To 20ul
Incubate at 50℃ for 1 hour
DNA ligation with T4 DNA Ligase
Name Type Description Designer Length
XylS BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO Pseudomonas putida xxxxxxxx xxxxxxxxxxx
XylS BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO Pseudomonas putida xxxxxxxx xxxxxxxxxxx
XylS BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO Pseudomonas putida xxxxxxxx xxxxxxxxxxx
XylS BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO Pseudomonas putida xxxxxxxx xxxxxxxxxxx
XylS BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO Pseudomonas putida xxxxxxxx xxxxxxxxxxx
XylS BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO Pseudomonas putida xxxxxxxx xxxxxxxxxxx
XylS BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO Pseudomonas putida xxxxxxxx xxxxxxxxxxx
XylS BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO Pseudomonas putida xxxxxxxx xxxxxxxxxxx
XylS BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO Pseudomonas putida xxxxxxxx xxxxxxxxxxx
XylS BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO Pseudomonas putida xxxxxxxx xxxxxxxxxxx

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