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Team:CityU HK/notebook/safety
From 2014.igem.org
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<h2 id="sub">Environmental Safety</h2> | <h2 id="sub">Environmental Safety</h2> | ||
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| - | Possible risks: Spillage of bacterial cultures or organic wastes/solutions <br> | + | Possible risks <b>:</b> Spillage of bacterial cultures or organic wastes/solutions <br> |
Solution: Spillages will be immediately dealt with in the lab using appropriate disinfection procedures (e.g. disinfection with antibacterial detergents) and wipe down benches surface with 70% ethanol.<br><br> | Solution: Spillages will be immediately dealt with in the lab using appropriate disinfection procedures (e.g. disinfection with antibacterial detergents) and wipe down benches surface with 70% ethanol.<br><br> | ||
Revision as of 14:13, 23 September 2014
Safety
The organisms we used for this project :
Species |
Strain no. / name |
Risk Group |
Any risk to humans? |
|
E. coli K 12 |
DH5α |
1 |
E. coli K-12 is considered as having no or low individual and community risk. It is unlikely to cause human or animal disease. |
The organisms we used for this project :
Part number |
Source of the physical DNA |
What species does this part originally come from ? |
Risk Group |
The function of the parts |
|
BBa_K1472601 ('TesA) |
City University of Hong Kong |
Escherichia coli K 12 |
1 |
A protein that catalyzes the conversion of fatty acyl-ACP (acyl carrier protein) or fatty acyl-CoA to free fatty acids |
|
BBa_K1472606 (FadD & FadL) |
City University of Hong Kong |
Escherichia coli K 12 |
1 |
A TetR repressible construct encoding the FadD and FadL intermembrane proteins. |
|
BBa_K?????? |
Life Technologies Limited |
Synechocystis sp. PCC6803 |
1 |
A DNA construct containing △9 desaturase, △12 desaturase and △15 desaturase that work together to convert saturated fatty acid into ALA |
General public Safety :
All E. coli strains used in this project are attenuated mutant strains that are unlikely to survive/propagate in the environment outside the laboratory as they harbour many genetic mutations in their genome.
To reduce the risk of biological materials escaping from the lab, all bacterial strains are kept and stored in designated refrigerators (before and after all experiments) in the supervisor’s (Dr. Richard Kong) laboratory which is not accessible to the general public. The lab where iGEM experiments are conducted is also not accessible by people of the general public because all lab workers need to be authorized to work in the lab and are required to carry a “Name badge”.
Environmental Safety
Possible risks : Spillage of bacterial cultures or organic wastes/solutions
Solution: Spillages will be immediately dealt with in the lab using appropriate disinfection procedures (e.g. disinfection with antibacterial detergents) and wipe down benches surface with 70% ethanol.
Organic solvents, biological materials and wastes (microorganisms) and plastic-wares are collected in separate waste containers for collection by technical staff. Biological wastes are treated with bleach and detergent and subsequently autoclaved to kill off all live microorganisms.
