Team:Caltech/week13
From 2014.igem.org
(Difference between revisions)
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<br>Preparation of fsr system for LC/MS analysis: | <br>Preparation of fsr system for LC/MS analysis: | ||
<ul><li>Set up 0 nM, 250 nM, & 500 nM aTc inductions of pTG005-unFLAG expressing cells in MOPS media. 5 μL aliquots of last night's liquid culture was used to start each culture.</li> | <ul><li>Set up 0 nM, 250 nM, & 500 nM aTc inductions of pTG005-unFLAG expressing cells in MOPS media. 5 μL aliquots of last night's liquid culture was used to start each culture.</li> | ||
+ | <li>Made a glycerol stock of pTG005-noFLAG</li> | ||
</ul> | </ul> | ||
<b>lamBCDA & fsrABC Reception Systems</b> | <b>lamBCDA & fsrABC Reception Systems</b> | ||
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<td valign="top"> | <td valign="top"> | ||
<b>Export Systems</b> | <b>Export Systems</b> | ||
- | < | + | <br>Preparation of fsr system for LC/MS analysis: |
- | + | <ul><li>Vacufuged pTG005-unFLAG acetonitrile samples to completion.</li> | |
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</ul> | </ul> | ||
</td> | </td> |
Latest revision as of 01:34, 15 September 2014
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