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Team:UCL/Science/Experiment
From 2014.igem.org
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<a href="/Team:UCL/protocols"><span class="label-warning">digest</span></a> | <a href="/Team:UCL/protocols"><span class="label-warning">digest</span></a> | ||
<a href="/Team:UCL/protocols"><span class="label-warning">gel</span></a></p> | <a href="/Team:UCL/protocols"><span class="label-warning">gel</span></a></p> | ||
| - | < | + | <h4>Background</h4> |
<p>Content</p> | <p>Content</p> | ||
| - | < | + | <h4>Results</h4> |
<p>Content Here</p> | <p>Content Here</p> | ||
| - | < | + | <h4>Conclusion</h4> |
<p>Content Here</p> | <p>Content Here</p> | ||
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<a href="/Team:UCL/protocols"><span class="label-warning">gel</span></a></p> | <a href="/Team:UCL/protocols"><span class="label-warning">gel</span></a></p> | ||
<br> | <br> | ||
| - | < | + | <h4>Background</h4> |
<br> | <br> | ||
<p>Performed transformation of the following BioBricks using non-commercial competent cells:</p> | <p>Performed transformation of the following BioBricks using non-commercial competent cells:</p> | ||
<br> | <br> | ||
| - | < | + | <ol> |
| - | < | + | <li>BBa_J23119</li> |
| - | < | + | <li>BBa_R0010</li> |
| - | < | + | <li>BBa_R0011</li> |
| - | < | + | <li>BBa_K206000</li> |
| - | < | + | <li>BBa_B0034</li> |
| - | < | + | <li>BBa_B0012</li> |
| - | < | + | <li>BBa_J04450</li> |
| - | < | + | <li>BBa_E2050</li> |
| + | </ol> | ||
<p>Performed transformations of Lisbon plasmids using NEB competent cells (c2987):</p> | <p>Performed transformations of Lisbon plasmids using NEB competent cells (c2987):</p> | ||
| + | <ol> | ||
| + | <li>pPAzoR - P. putida Azo reductase</li> | ||
| + | <li>pCotA - Laccase</li> | ||
| + | <li>pPpDyP - P.Putida dye decolourizing peroxidase</li> | ||
| + | <li>pBsDyP - B. subtilis dye decolourizing peroxidase</li> | ||
| + | <li>p1B6 - Azoreductase 1B6 (mutated from AzoR)</li> | ||
| + | </ol> | ||
| + | <h4>Results</h4> | ||
<br> | <br> | ||
| - | + | <p>Lisbon plasmid transformation using NEB competent cells successfully grew when plated with the appropriate antibiotic</p> | |
| - | + | <p>BioBrick plasmid transformations were successful, however the colonies had grown as a lawn making it difficult to pick a single colony.</p> | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | <p>Lisbon plasmid transformation using NEB competent cells successfully grew when plated with the appropriate antibiotic<p | + | |
| - | + | ||
| - | <p>BioBrick plasmid transformations were successful, however the colonies had grown as a lawn making it difficult to pick a single colony.<p> | + | |
<br> | <br> | ||
| - | < | + | <h4>Conclusion</h4> |
<p>Made overnight culture of lisbon plasmids, preparing to keep in glycerol stocks</p> | <p>Made overnight culture of lisbon plasmids, preparing to keep in glycerol stocks</p> | ||
Revision as of 16:11, 8 September 2014
Experiments
Laboratory Team
List of Experiments
- Experiment 01: Extraction of useful BioBrick plasmids from iGEM 2014 Distribution Kit
- Experiment 02: Transforming E. coli with Azo-reductase plasmids
- Experiment 03: Diagnostic digest of azo-reductase plasmids
- Experiment 04: Creation of azo-reductase BioBrick parts from plasmids
- Experiment 05: Diagnostic digest of azo-reductase BioBrick parts
- Experiment 06: Assembling azo-reductase BioBrick Device(s)
- Experiment 07: Characterisation of azo-reductase BioBrick devices
Experiment 1: Extraction of useful BioBrick plasmids from iGEM 2014 Distribution Kit - Date: [ddmmyy]
Protocols
competent cells
transformation
miniprep
digest
gel
Background
Content
Results
Content Here
Conclusion
Content Here
Experiment 2: Transforming E. coli with Azo-reductase plasmids - Date: [ddmmyy]
Protocols
competent cells
transformation
miniprep
digest
gel
Background
Performed transformation of the following BioBricks using non-commercial competent cells:
- BBa_J23119
- BBa_R0010
- BBa_R0011
- BBa_K206000
- BBa_B0034
- BBa_B0012
- BBa_J04450
- BBa_E2050
Performed transformations of Lisbon plasmids using NEB competent cells (c2987):
- pPAzoR - P. putida Azo reductase
- pCotA - Laccase
- pPpDyP - P.Putida dye decolourizing peroxidase
- pBsDyP - B. subtilis dye decolourizing peroxidase
- p1B6 - Azoreductase 1B6 (mutated from AzoR)
Results
Lisbon plasmid transformation using NEB competent cells successfully grew when plated with the appropriate antibiotic
BioBrick plasmid transformations were successful, however the colonies had grown as a lawn making it difficult to pick a single colony.
Conclusion
Made overnight culture of lisbon plasmids, preparing to keep in glycerol stocks
Experiment 3: Diagnostic digest of azo-reductase plasmids - Date: [ddmmyy]
Protocols
competent cells
transformation
miniprep
digest
gel
senectus et netus et malesuada
Experiment 4: Creation of azo-reductase BioBrick parts from plasmids - Date: [ddmmyy]
Protocols
competent cells
transformation
miniprep
digest
gel
senectus et netus et malesuada
Experiment 5: Diagnostic digest of azo-reductase BioBrick parts - Date: [ddmmyy]
Protocols
competent cells
transformation
miniprep
digest
gel
senectus et netus et malesuada
Experiment 6: Assembling azo-reductase BioBrick Device(s) - Date: [ddmmyy]
Protocols
competent cells
transformation
miniprep
digest
gel
senectus et netus et malesuada
Experiment 7: Characterisation of azo-reductase BioBrick devices - Date: [ddmmyy]
Protocols
competent cells
transformation
miniprep
digest
gel
senectus et netus et malesuada
