Team:ITESM-Guadalajara

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Profile</td>
 
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        <td data="#project" class="menu-item">Project</td>
 
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item">Attributions</td>
 
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href="https://2014.igem.org/Main_Page"><img
 
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    <h1><b1><font size=20>Bio San</font></b></h1>
 
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    Chitosan is a polysaccharide found in abundance in shrimp
 
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shell. The current extraction methods involve the use of strong
 
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acids and alkalis, these substances are dangerous wastes that can
 
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compromise the integrity of soil and water where they are
 
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disposed.
 
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    <br><br>Due to its stability and biocompatibility, chitosan
 
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has been widely used in pharmaceutical, food, and cosmetic
 
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industries, among many others. The Chitosan global market has an
 
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estimated value of $20 billion USD. Particularly, the
 
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pharmaceutical industry represents on average 1.2% of total GDP
 
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in Mexico and has grown at a 10% rate since 2008.
 
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    <br><br>Therefore, it is imperative to develop a new and
 
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efficient extraction process, that is also eco-friendly. We’ve
 
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developed a new process that consists in the pretreatment of
 
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shrimp shells, followed by a fermentation in which it is
 
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demineralized and deproteinized by B. subtilis. Finally, it goes
 
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through a deacetylation process in a fermentation performed by
 
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our genetically modified E. coli strain. This modified bacterium
 
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contains 6 different enzymes that are found naturally in
 
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microorganisms that are capable of cutting and deacetylating
 
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chitin for the generation of chitosan. The action of these
 
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enzymes and a careful temperature treatment will allow us to
 
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generate chitosan with a 90% deacetylation degree. This
 
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deacetylation
 
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degree is the required one by the pharmaceutical industry, our
 
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target market.
 
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          <tr><td>Sofy</td></tr>
 
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          <tr><td>Rodolfo</td></tr>
 
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          <tr><td>Maggie</td></tr>
 
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          <tr><td>Nombre</td></tr>
 
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        <table class="team-member-footer">
 
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          <tr><td>Luis</td></tr>
 
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  <div id="teamprofile" class="container">
 
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    Team Profile section
 
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  <div id="project" class="container">
 
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    Project section
 
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  <div id="parts" class="container">
 
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    Parts section
 
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  <div id="modeling" class="container">
 
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    Modeling section
 
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  <div id="notebook" class="container">
 
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    Notebook section
 
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  <div id="safety" class="container">
 
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    Safety section
 
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  <div id="attributions" class="container">
 
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    Attributions section
 
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<p style="color:#E7E7E7"> <a
 
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href="https://2014.igem.org/wiki/index.php?title=Team:ITESM-
 
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Guadalajara&action=edit"style="color:#190718"> Click here  to
 
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edit this page!</a> </p>
 

Revision as of 05:31, 5 September 2014