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Team:TU Eindhoven/Project/protocols
From 2014.igem.org
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<span>genetic engineering protocols</span> | <span>genetic engineering protocols</span> | ||
| - | <br> | + | <br> <p class="para">The following protocols are used in the Biolab during the modification of bacteria. |
| - | <p class="para"><a href="https://static.igem.org/mediawiki/2014/6/6e/Protocol_-_Colony_PCR.pdf">Colony PCR</a | + | |
| + | <br><br> | ||
| + | |||
| + | </p> | ||
| + | <p class="para"><a target="_blank" href="https://static.igem.org/mediawiki/2014/6/6e/Protocol_-_Colony_PCR.pdf">Colony PCR</a> | ||
| - | + | <a target="_blank" href="https://static.igem.org/mediawiki/2014/b/b1/Protocol_-_FACS_%28Antibody_Titration%29.pdf">Antibody Titration with FACS</a> | |
| - | <a href="https://static.igem.org/mediawiki/2014/b/b1/Protocol_-_FACS_%28Antibody_Titration%29.pdf">Antibody Titration with FACS</a>< | + | <br><br> |
| - | <a href="https://static.igem.org/mediawiki/2014/7/7e/Protocol_-_FACS_%28DBCO-56-TAMRA%29.pdf">FACS for sorting with DBCO-TAMRA</a>< | + | <a target="_blank" href="https://static.igem.org/mediawiki/2014/7/7e/Protocol_-_FACS_%28DBCO-56-TAMRA%29.pdf">FACS for sorting with DBCO-TAMRA</a> |
| + | <br><br> | ||
| - | <a href="https://static.igem.org/mediawiki/2014/f/f0/Protocol_-_FACS_%28DBCO-PEG_10_kDa%29.pdf">FACS for sorting with DBCO-PEG(10kDa)</a>< | + | <a target="_blank" href="https://static.igem.org/mediawiki/2014/f/f0/Protocol_-_FACS_%28DBCO-PEG_10_kDa%29.pdf">FACS for sorting with DBCO-PEG(10kDa)</a> |
| + | <br><br> | ||
| - | <a href="https://static.igem.org/mediawiki/2014/a/ae/Protocol_-_Insert_%2B_Vector_Ligation.pdf">Vector Ligation</a></p> | + | <a target="_blank" href="https://static.igem.org/mediawiki/2014/a/ae/Protocol_-_Insert_%2B_Vector_Ligation.pdf">Vector Ligation</a></p> |
| - | <a href="https://static.igem.org/mediawiki/2014/8/81/Protocol_-_Overhang_PCR.pdf">Overhang PCR</a></p> | + | <a target="_blank" href="https://static.igem.org/mediawiki/2014/8/81/Protocol_-_Overhang_PCR.pdf">Overhang PCR</a></p> |
| - | <a href="https://static.igem.org/mediawiki/2014/0/0a/Protocol_-_PCR_Purification_of_Insert_Fragment.pdf">PCR purification of DNA fragments</a></p> | + | <a target="_blank" href="https://static.igem.org/mediawiki/2014/0/0a/Protocol_-_PCR_Purification_of_Insert_Fragment.pdf">PCR purification of DNA fragments</a></p> |
| - | <a href="https://static.igem.org/mediawiki/2014/2/22/Protocol_-_Plasmid_Amplification.pdf">Plasmid Amplification</a></p> | + | <a target="_blank" href="https://static.igem.org/mediawiki/2014/2/22/Protocol_-_Plasmid_Amplification.pdf">Plasmid Amplification</a></p> |
| - | <a href=" | + | <a target="_blank" href=" |
https://static.igem.org/mediawiki/2014/0/07/Protocol_-_Plasmid_and_gene_digestion.pdf">Plasmid Gene Digestion</a></p> | https://static.igem.org/mediawiki/2014/0/07/Protocol_-_Plasmid_and_gene_digestion.pdf">Plasmid Gene Digestion</a></p> | ||
| - | <a href=" | + | <a target="_blank" href=" |
https://static.igem.org/mediawiki/2014/2/29/Protocol_-_Plasmid_purification.pdf">Plasmid Purification</a></p> | https://static.igem.org/mediawiki/2014/2/29/Protocol_-_Plasmid_purification.pdf">Plasmid Purification</a></p> | ||
| - | <a href=" | + | <a target="_blank" href=" |
https://static.igem.org/mediawiki/2014/1/13/Protocol_-_Preparative_steps.pdf">Preparative Steps</a></p> | https://static.igem.org/mediawiki/2014/1/13/Protocol_-_Preparative_steps.pdf">Preparative Steps</a></p> | ||
| - | <a href=" | + | <a target="_blank" href=" |
https://static.igem.org/mediawiki/2014/f/f8/Protocol_-_Protein_Expression_Curve.pdf">Protein Expression Curve</a></p> | https://static.igem.org/mediawiki/2014/f/f8/Protocol_-_Protein_Expression_Curve.pdf">Protein Expression Curve</a></p> | ||
| - | <a href=" | + | <a target="_blank" href=" |
https://static.igem.org/mediawiki/2014/4/45/Protocol_-_Protein_expression.pdf">Protein Expression</a></p> | https://static.igem.org/mediawiki/2014/4/45/Protocol_-_Protein_expression.pdf">Protein Expression</a></p> | ||
| - | <a href=" | + | <a target="_blank" href=" |
https://static.igem.org/mediawiki/2014/6/62/Protocol_-_Site_Directed_Mutagenesis.pdf">Site Directed Mutagenesis</a></p> | https://static.igem.org/mediawiki/2014/6/62/Protocol_-_Site_Directed_Mutagenesis.pdf">Site Directed Mutagenesis</a></p> | ||
| - | <a href=" | + | <a target="_blank" href=" |
| - | https://static.igem.org/mediawiki/2014/b/b5/Protocol_-_Transformation.pdf">Transformation</a></p> | + | https://static.igem.org/mediawiki/2014/b/b5/Protocol_-_Transformation.pdf">Vector Transformation</a></p> |
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<span>microfluidics protocols</span> | <span>microfluidics protocols</span> | ||
| - | <p class="para">Here will follow all | + | <p class="para">Here will follow all microfluidic engineering protocols</p> |
</div> | </div> | ||
<div class="clear"></div> | <div class="clear"></div> | ||
Latest revision as of 19:19, 31 August 2014
Protocols
Introduction
For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.
genetic engineering protocols
The following protocols are used in the Biolab during the modification of bacteria.
Colony PCR
Antibody Titration with FACS
FACS for sorting with DBCO-TAMRA
FACS for sorting with DBCO-PEG(10kDa)
Vector Ligation
chemistry protocols
microfluidics protocols
Here will follow all microfluidic engineering protocols
