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Team:TU Eindhoven/Project/protocols
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<span>genetic engineering protocols</span> | <span>genetic engineering protocols</span> | ||
| - | <br> | + | <br> <p class="para">The following protocols are used in the Biolab during the modification of bacteria. |
| - | <p class="para">Colony PCR</p> | + | |
| + | <br><br> | ||
| + | |||
| + | </p> | ||
| + | <p class="para"><a target="_blank" href="https://static.igem.org/mediawiki/2014/6/6e/Protocol_-_Colony_PCR.pdf">Colony PCR</a> | ||
| + | |||
| + | <a target="_blank" href="https://static.igem.org/mediawiki/2014/b/b1/Protocol_-_FACS_%28Antibody_Titration%29.pdf">Antibody Titration with FACS</a> | ||
| + | <br><br> | ||
| + | |||
| + | <a target="_blank" href="https://static.igem.org/mediawiki/2014/7/7e/Protocol_-_FACS_%28DBCO-56-TAMRA%29.pdf">FACS for sorting with DBCO-TAMRA</a> | ||
| + | <br><br> | ||
| + | |||
| + | <a target="_blank" href="https://static.igem.org/mediawiki/2014/f/f0/Protocol_-_FACS_%28DBCO-PEG_10_kDa%29.pdf">FACS for sorting with DBCO-PEG(10kDa)</a> | ||
| + | <br><br> | ||
| + | |||
| + | <a target="_blank" href="https://static.igem.org/mediawiki/2014/a/ae/Protocol_-_Insert_%2B_Vector_Ligation.pdf">Vector Ligation</a></p> | ||
| + | |||
| + | <a target="_blank" href="https://static.igem.org/mediawiki/2014/8/81/Protocol_-_Overhang_PCR.pdf">Overhang PCR</a></p> | ||
| + | |||
| + | <a target="_blank" href="https://static.igem.org/mediawiki/2014/0/0a/Protocol_-_PCR_Purification_of_Insert_Fragment.pdf">PCR purification of DNA fragments</a></p> | ||
| + | |||
| + | <a target="_blank" href="https://static.igem.org/mediawiki/2014/2/22/Protocol_-_Plasmid_Amplification.pdf">Plasmid Amplification</a></p> | ||
| + | <a target="_blank" href=" | ||
| + | https://static.igem.org/mediawiki/2014/0/07/Protocol_-_Plasmid_and_gene_digestion.pdf">Plasmid Gene Digestion</a></p> | ||
| + | |||
| + | <a target="_blank" href=" | ||
| + | https://static.igem.org/mediawiki/2014/2/29/Protocol_-_Plasmid_purification.pdf">Plasmid Purification</a></p> | ||
| + | |||
| + | <a target="_blank" href=" | ||
| + | https://static.igem.org/mediawiki/2014/1/13/Protocol_-_Preparative_steps.pdf">Preparative Steps</a></p> | ||
| + | |||
| + | <a target="_blank" href=" | ||
| + | https://static.igem.org/mediawiki/2014/f/f8/Protocol_-_Protein_Expression_Curve.pdf">Protein Expression Curve</a></p> | ||
| + | |||
| + | <a target="_blank" href=" | ||
| + | https://static.igem.org/mediawiki/2014/4/45/Protocol_-_Protein_expression.pdf">Protein Expression</a></p> | ||
| + | <a target="_blank" href=" | ||
| + | https://static.igem.org/mediawiki/2014/6/62/Protocol_-_Site_Directed_Mutagenesis.pdf">Site Directed Mutagenesis</a></p> | ||
| + | |||
| + | <a target="_blank" href=" | ||
| + | https://static.igem.org/mediawiki/2014/b/b5/Protocol_-_Transformation.pdf">Vector Transformation</a></p> | ||
| + | |||
| + | |||
| + | |||
</div> | </div> | ||
| - | + | ||
<div class="clear"></div> | <div class="clear"></div> | ||
</div> | </div> | ||
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<span>chemistry protocols</span> | <span>chemistry protocols</span> | ||
| - | <p class="para"> | + | <p class="para"> |
| + | |||
| + | <a href=" | ||
| + | https://static.igem.org/mediawiki/2014/3/3d/Protocol_-_Monitoring_SPAAC_with_UVVIS.pdf">SPAAC reaction monitoring with UV-Visible Spectroscopy</a></p> | ||
| + | |||
| + | |||
</div> | </div> | ||
<div class="clear"></div> | <div class="clear"></div> | ||
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<span>microfluidics protocols</span> | <span>microfluidics protocols</span> | ||
| - | <p class="para">Here will follow all | + | <p class="para">Here will follow all microfluidic engineering protocols</p> |
</div> | </div> | ||
<div class="clear"></div> | <div class="clear"></div> | ||
Latest revision as of 19:19, 31 August 2014
Protocols
Introduction
For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.
genetic engineering protocols
The following protocols are used in the Biolab during the modification of bacteria.
Colony PCR
Antibody Titration with FACS
FACS for sorting with DBCO-TAMRA
FACS for sorting with DBCO-PEG(10kDa)
Vector Ligation
chemistry protocols
microfluidics protocols
Here will follow all microfluidic engineering protocols
