"
Team:Evry/Notebook/Sensing/PCBs/week6
From 2014.igem.org
(Difference between revisions)
| Line 10: | Line 10: | ||
<p> | <p> | ||
| - | |||
<h4> 08/4/14 :</h4> | <h4> 08/4/14 :</h4> | ||
<h4> 08/5/14 :</h4> | <h4> 08/5/14 :</h4> | ||
| + | <u>Sensor construction hbpR/PhbpC</u> | ||
<div class="center"> | <div class="center"> | ||
<div class="thumb tnone"> | <div class="thumb tnone"> | ||
| Line 44: | Line 44: | ||
<br/> Plasmid purification with NucleoSpin Plasmid (Macherey-Nagel) protocol: 487,9 ng/µL | <br/> Plasmid purification with NucleoSpin Plasmid (Macherey-Nagel) protocol: 487,9 ng/µL | ||
</p> | </p> | ||
| + | |||
| + | <u>Sensor construction hbpR/PhbpC</u> | ||
<h4> 08/9/14 :</h4> | <h4> 08/9/14 :</h4> | ||
Latest revision as of 16:04, 19 August 2014
Week 6
08/4/14 :
08/5/14 :
Sensor construction hbpR/PhbpC
08/6/14 :
Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:Plate on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp) and incubation overnight at 37°C
08/7/14 :
Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:Culturing of 1 colony in 5mL of LB + 5µL Amp and incubation at 37°C, 200rpm
08/8/14 :
Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:Plasmid purification with NucleoSpin Plasmid (Macherey-Nagel) protocol: 487,9 ng/µL Sensor construction hbpR/PhbpC
