Team:HokkaidoU Japan

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Anti-sense RNA (asRNA) is a small non-coding RNA which has a complementary sequence with a certain messenger RNA, and inhibits the translation of the mRNA by RNA pairing. Utilization of anti-sense RNA (asRNA) is becoming more popular method for silencing of target gene expression even among iGEMers. However, it is difficult to find out the most efficient asRNA structure, since the efficiency of an asRNA may be affected by several essential elements, (1) its secondary structures, (2) its stability in cells, (3) stability of asRNA-mRNA duplex, and (4) its annealing position with mRNA etc.</ br>
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Aim of our project is directed to generate a standard protocol for finding efficient asRNA structure, which has simple paired-termini (PT) structure and is suited to the BioBrick standard. PT flanks anti-sense sequence and stabilizes asRNA by forming “stem-loop” structure in E. coli (N. Nakashima et al., 2006). We also make a protocol for designing the best anti-sense sequence against any mRNA. We believe that our products will help many iGEMers and whom considering of asRNA.</ br>
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In our Human Practice, we are planning to give a presentation about synthetic biology and the activity of iGEM in our “University Festival” and our “Visit high schools”. In every event, we do an interview survey to know what opinions about genetic recombination younger students or people have. Then, we make some helps to get them right knowledge about gene recombination through relationship with us. On our activities, we hope that this project contribute to lead young high school students to accept genetic engineering and to change future public acceptance of genetic engineering.
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<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Background">Overview</a></li>
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<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Background">Background</a></li>
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<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/H_Stem">H-stem System</a></li>
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                                                        <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/H_Stem#Overview">Overview</a></li>
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                                                        <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/H_Stem#asRNA_stabilization">Stabilization</a></li>
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                                                        <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/H_Stem#Modelling">Modelling</a></li>
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                                                        <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/H_Stem#Construct">Construct</a></li>
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<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/asB0034">Anti-sense B0034</a></li>
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            <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/asB0034">Overview</a></li>
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                                                            <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/asB0034#Results">Results</a></li>
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                                                            <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/asB0034#Conclusion">Conclusion</a></li>
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<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Length">Length Variation</a></li>
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<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Materials">Extra Materials</a></li>
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<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Evaluation">Evaluation</a></li>
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<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/About_Us#Acknowledgements">Acknowledgements</a></li>
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<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/About_Us#Team">Team</a></li>
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<h1 style="font-family: 'Special Elite', cursive; font-size: 48px;">~ RNA in love ~</h1>
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<h3 style="font-family: 'Special Elite', cursive; font-size: 24px;">This year iGEM HokkaidoU invites you to a lovely story ...</h3>
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This is a love story of one shy girl called Annie. She was very hesitant, but her passion for him was second to none. One day, Cupid found Annie and helped her. Well, how would the future of her romance going to be?
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Annie / asRNA
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The shiest girl in the village, but has a feeling to Mike in secrecy.
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Mike / mRNA
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The most popular boy in the village, but a little indecisive.
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Rachel / ribosome
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The most confident girl in the village. She loves Mike and approaches him positively.
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Cupid / HokkaidoU
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A wonderful sprite in the village. He sometimes helps a villager whimsically.
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Once upon a time, there was a girl, Annie. She fell in love with Mike who was the darling of the town. But she had a formidable rival, Rachel. She was very positive to her love so that she always wanted to monopolize Mike.
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Contrasting to her, as Annie was too shy, it was difficult for her only to approach to Mike. Nevertheless, she never stopped thinking about Mike. Cupid found such poor Annie.
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As Cupid wanted to help Annie’s love, he casted a spell over her. This was the wonderful magic STEM. Enchanted, her clothes turned into greatly beautiful clothes.
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She was very surprised to look at herself in a mirror because the girl in a mirror appeared like not herself to her. Owing to wonderful magic, she was able to have confidence and a little courage to go to Mike.
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Mike fell in love with changed Annie at a first sight.
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“Just a small change of myself makes my world more wonderful than I have dreamed ever.”
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Although Annie couldn’t hide her surprise, she felt very happy.
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And then, Annie and Mike were together happily forever. 
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Latest revision as of 15:20, 9 September 2015

~ RNA in love ~

This year iGEM HokkaidoU invites you to a lovely story ...

This is a love story of one shy girl called Annie. She was very hesitant, but her passion for him was second to none. One day, Cupid found Annie and helped her. Well, how would the future of her romance going to be?

Annie / asRNA

The shiest girl in the village, but has a feeling to Mike in secrecy.

Mike / mRNA

The most popular boy in the village, but a little indecisive.

Rachel / ribosome

The most confident girl in the village. She loves Mike and approaches him positively.

Cupid / HokkaidoU

A wonderful sprite in the village. He sometimes helps a villager whimsically.

Once upon a time, there was a girl, Annie. She fell in love with Mike who was the darling of the town. But she had a formidable rival, Rachel. She was very positive to her love so that she always wanted to monopolize Mike.
Contrasting to her, as Annie was too shy, it was difficult for her only to approach to Mike. Nevertheless, she never stopped thinking about Mike. Cupid found such poor Annie.
As Cupid wanted to help Annie’s love, he casted a spell over her. This was the wonderful magic STEM. Enchanted, her clothes turned into greatly beautiful clothes.
She was very surprised to look at herself in a mirror because the girl in a mirror appeared like not herself to her. Owing to wonderful magic, she was able to have confidence and a little courage to go to Mike.
Mike fell in love with changed Annie at a first sight. “Just a small change of myself makes my world more wonderful than I have dreamed ever.” Although Annie couldn’t hide her surprise, she felt very happy. And then, Annie and Mike were together happily forever.

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