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BUCT-CHINA

Project

Overview

There are a population of 884 million still drink water without the previous purification all over the world...

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Biosensors mining

Quorum sensing was first found in Vibrio fischeri in the ocean. Microorganisms achieve information communication by ...

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Biosensors

Adoption of luminescent bacteria for air pollution monitoring, antibiotics screening and rapid...

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Device

This device includes two parts, one of them is physical instrument and the other is matched software...

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overview

There are a population of 884 million still drink water without the previous purification all over the world. There are about 3 billion to 4 billion families lack of drinkable water. Each year about 3.5 million people's deaths are associated with insufficient water supply and sanitation condition, which occurs mainly in developing countries. According to all above, the matter of water quality is a great matter of human beings’ life quality. It has always been crucial to many researchers engaging in improving and making breakthroughs on the approaches towards water quality detection. Currently chemical detection methods are more complex and time consuming; besides ,on the way of bringing the samples to the laboratory, some of the indicators may probably be changed. Another thing we may acknowledge is that chemical detection apparatus cost too much money. Based on the matters above, this year we intended to look for a new way using biotechnology. Here come the ideas, through two kinds of approaches we may achieve rapid water quality detection. One of the approaches is so called comprehensive detection, here we mainly rely on LuxAB ,which encodes a kind of luciferase. Because anything toxic within the water may have a negative impact on the activity of luciferase, the luminescence is likely to be decreased. The other is specific detection, because only in this way can we successfully quantify specific metal ions like Hg(II). One sequence of MerR operon occupies a significant position along with quorum sensing system. In addition, we use gfp as reporter. Besides the two biosensors we built, the joint uses of microfluidic chips, automatic detection devices, photoelectric detection technology and micro total analysis systems will extremely promote biological detection method. This device can also be linked to the computer and has the ability of offline CNC screen operation, which improves the detection efficiency. What’s more, little device is convenient to carry and free from limitation of experimental conditions. Biological method not only has the priority of concise operations and less consumption, but also has the advantages of high accuracy, specificity, and security.

Biosensors Mining

Quorum sensing was first found in Vibrio fischeri in the ocean. Microorganisms achieve information communication by releasing a signaling molecules referred as auto inducer(AI). AI will promotes related gene expression in bacteria, meanwhile regulate microbes’ biological behaviors. Bacteria use quorum sensing to coordinate certain behaviors such as biofilm formation, virulence, bioluminescence and antibiotic resistance, based on the local density of bacteria. Thus microbes can collaborate to achieve cell populations’s engineering. In Vibrio fischeri , plux promoter regulates the expression of quorum sensing system while plux promoter is automatically regulated by AI. PluxL and pluxR respectively promote transcription to the left and right. PluxL regulates transcription of LuxR gene ;in the meantime, pluxR can also start expression of luxI and fluorescence related genes. LuxI, is responsible for production of N-acyl-homoserine-lactone (AHL) autoinducer while luxR,is activated by this autoinducer to increase transcription of the luciferase operon. The enhanced E.coli quorum sensing system we reconstructed is greatly dependent to plac promoter. Plac will serve as pluxL in this system to promote transcription. Once the transcription has been started by plac, it will extend to series genes such as luxR、pluxR and luxI downstream. Noticeably, the LuxI/LuxR genes form a functional pair, considering LuxI as the auto-inducer synthase and LuxR as the receptor. We can obtain translation products luxI to catalyze the formation of AHL. The yield of luxI protein is very low with a tenuous concentration of cells, likewise result in the subtle concentration of AHL in periplasmic. As the amount of cells increases, the density of AHL will reach a threshold value, this can strongly accelerate the formation of one kind of complex which is composed of AHL and luxR , also the complex will firmly promotes transcription of pluxR ,hence we acquire desired expression of bioluminescence related genes as well as increasing amount of luxI、luxR、AHL, which in turn form a positive regulation. In addition, plac is a fully functional part which act as a substitution to pluxL, preventing pluxL from inhibition of the AHL-LuxR complex, what’s more, releasing the negative influence on the expression of LuxR. Based on this enhanced E.coli quorum sensing system, we got a innovative method for specific metal ions detection. The highlight is that we combine MerR and relevant promoter sequence pMerT to the downstream of luxI. The MerR family of metal-binding, metal-responsive proteins is unique in that they activate transcription from unusual promoters and coordinate metals through cysteine residues. They have conserved primary structures yet can effectively discriminate metals in vivo. Expression of MerR (in the absence of MerR and Hg (II) in the cell) proceeds from the PmerR promoter. In the absence of Hg(II), the MerR homodimer binds to the operator region within the divergent promoter with binding centered on the dyad symmetrical DNA sequence between the -35 and -10 sequences of PmerT, slightly repressing transcription of the structural gene promoter, and repressing transcription of merR from the PmerR promoter. Once the MerR homodimer has bound to merOP, recruitment of RNAP to the mer promoter occurs , and MerR has been shown to cross-link to several subunits of RNAP . In the presence of mercuric ions, one Hg(II) per MerR homodimer coordinates in a trigonal manner to three essential cysteine residues of the MerR homodimer, two cysteines from one monomer, and one from the other. Hg(II) binding to the MerR homodimer results in both a relaxation of the DNA bends induced by apo-MerR, and both DNA distortion and an allosteric under winding of the promoter sequence by approximately 33. The under winding of the promoter DNA aligns the -10 and -35 sequences, such that RNA polymerase can recognize and bind to these sites, initiating transcription from PmerT . The reporter gene we apply is gfp ,which also depends on the transcription of the same promoter pMerT. According to the design, we intended to make best use of the recombinant E.coli quorum sensing system to increase the bacteria density to a threshold value and produce a sufficient amount of AHL and LuxR protein, forming a continuous positive feedback. Meanwhile, there supposed to be a “switch” referred as MerR and pMerT. Only the presence of Hg(II) do drive the expression of structural genes and fluorescence related gene. Moreover, the fluorescence intensity should theoretically link a certain correlation to the concentration of Hg(II) in internal condition. For the purpose of continuous detection, what should not be ignored is that we desire enough MerR homodimer to bind to pMerT then interdict the transcription pathway. This clarifies the second aspect of recombinant E.coli quorum sensing system. Concluded, we designed this innovative system intending to get a fasten detection method towards specific metal ions in vivo.

Project: The influence of temperature on the engineering bacteria in the fermentation tank

23rd Mar 2014 Meet Up with BIT-iGEM

Encountered Problems:

1、 too complicated gene pathway to accomplish in a short period of time;

2、 the effects of Web site, poster and other artists are not so beautiful;

3、 time for Sponsorship and advertising is a bit tight;

4、 visa and travel arrangements should be considered early.

Notice:

1、 DNA distribution must be tested before you intend to use one of those parts;

2、 Meet up between teams and parts interchange needed to be paid attention to;

3、 Souvenirs are recommended;

4、 Preparation of team T-shirt and mascot;

5、 Pamphlets are really necessary , portable, convenient and practical .

Help Issues:

1、 the bacterial genes and genetic circuits implementation;

2、 some help about experimental skills and techniques;

3、 can provide temperature sensitive strains if needed.

Sensors

LuxAB---comprehensive biosensor

Luminescent Bacteria Method：

Adoption of luminescent bacteria for air pollution monitoring, antibiotics screening and rapid detection of water attracts wide attention in recent years. Luminescent bacteria is a kind of bacteria that can produce visible fluorescence in normal conditions, while its bioluminescence is produced by molecular oxygen and catalyzed by luciferase inside the cell. Luciferase oxidates flavin mononucleotide (FMNH2) and long chain fatty aldehyde in the reduction state to FMN and long chain fatty acid, at the same time emits blue-green light in 450 ~ 490 nm. Equation can be expressed as follows:

2、 the effects of Web site, poster and other artists are not so beautiful;

The process of luminescence involves a variety of substances, which are products of bacterial metabolism. When external condition worsens, its luminous way will be affected in a very short period of time, resulting in suppression or shutdown of luminescent reaction. As a result, luminescence of bacteria is positively correlated to the concentration of toxin in condition, what’s more, the correlation can be approached by a linear function. Based on the mechanism, luminescent bacteria have been widely adopted as a biological indicator in rapid quantification of toxin in the environment.

Luminescent bacteria luciferase gene

Bacteria luminescence is a consequence of its luciferase catalytic metabolic process. The luciferase encoding gene is LuxAB. All genus of luminescent bacteria have five core constitute lux gene operon, order of which is luxCDABE. Bacteria luciferase is composed of two subunits heterodimer, including luciferase alpha and beta subunits coded by luxA and luxB respectively. LuxC, luxD, luxE encode for reduction of fatty acid synthesis fatty aldehyde enzyme complexes of three subunits: reductase, transferase, synthetase, respectively. Mancin (1988) successfully cloned the photobacterium phosphoreum’s luciferase operon luxCDABFE and expressed it in Escherichia coli. Except for methods though plasmid, lux gene can also be integrated into the cell chromosome by genetic engineering techniques in pursuit of stability. What’s more, bioluminescent detection through lux gene is easy to achieve, and it is fast, simple and sensitive. Hence the bioluminescent detection method has extensive research and application in gene transduction, expression and regulation, environmental chemistry and its toxicity detection,etc.

Reference:

Shi J, Frymier P D. Toxicity of metals and organic chemicals evaluated with bioluminescence assays[J]. Chemosphere, 2005, 58: 543-550. Ru CX, Yang SD. Luminescent bacteria lux report gene system evaluation and application [J]. Microbiology, 2000, 27(4):297-299.

MerR ---Hg(II) Biosensor

Metals can pose a particular problem to bacteria,because essential metals may be limiting in the environment, requiring active uptake mechanisms to import minimum concentrations of essential metals (Butler 1998; Outten et al. 2000).Bacteria can encounter purely toxic metals, such as Hg, Cd and As, which have no beneficial role in cellular metabolism, and must be avoided, removed or neutralized metal. The key first step in how bacteria respond to varying levels of both toxic and essential metals in the internal environment of the cell is due to the metal sensor and regulator proteins that they encode. There are several known types of prokaryotic metal ion sensing regulators. These include the MerR and Fur families of regulators, the ArsR/SmtB family repressors and several other structural regulator types .

MerR – a mercury sensing gene activator

A key class of prokaryotic metal ion responsive activators is the MerR family, of which the mercuric ion sensing MerR is the archetype. The closely-related 144 amino-acid MerR proteins from the mercuric ion resistance (mer) operons from transposons Tn501 and Tn21 have been the most heavily studied of these proteins (reviewed in Summers 1992, 1986; Hobman & Brown 1997;Outten et al. 2000a; Barkay et al. 2003; Brown et al.2003, and references therein), and the mechanism of Hg(II) resistance is now well known (reviewed most recently by Barkay et al. (2003)). In the mer operon from Tn501, MerR binds to operator DNA within a divergent promoter and regulates both its own expression and expression of the downstream structural genes. The MerR family of metal-binding, metal-responsive proteins is unique in that they activate transcription from unusual promoters and coordinate metals through cysteine residues. They have conserved primary structures yet can effectively discriminate metals in vivo. Activation of transcription from the PmerT promoter by MerR in response to Hg(II) is hypersensitive (Ralston &O’Halloran 1990; Rouch et al. 1995), with virtually total induction of promoter activity occurring across a very narrow Hg(II) concentration rang. MerR has a strong selectivity for Hg(II) (Ralston & O’Halloran 1990)and Hg(II) has a very high affinity for MerR (Shewchuk et al. 1989b).

The model for regulation of the mer promoter

The current model for MerR activation of transcription proposes that expression of MerR (in the absence of MerR and Hg (II) in the cell) proceeds from the PmerR promoter. In the absence of Hg(II), the MerR homodimer binds to the operator region within the divergent promoter with binding centered on the dyad symmetrical DNA sequence between the -35 and -10 sequences of PmerT, slightly repressing transcription of the structural gene promoter, and repressing transcription of merR from the PmerR promoter. This is probably due to MerR interfering with RNA polymerase (RNAP) binding, or open complex formation. PmerT is unusual, it has a 19 bp spacing between the -35 and -10 sites , rather than the 16–18 bp spacing found in most prokaryotic promoters (Harley & Reynolds 1987). This makes PmerT suboptimal for RNAP recognition of, and binding to, the -35 and -10 sequences, preventing formation of the open complex and transcriptional activation (see Browning & Busby 2004). In the absence of Hg(II), the tight binding of the apo-MerR homodimer to PmerT causes a further bending of the promoter DNA to itself, making it even less ideal for RNAP binding (Ansari et al. 1995). Once the MerR homodimer has bound to merOP, recruitment of RNAP to the mer promoter occurs (Heltzel et al. 1990), and MerR has been shown to cross-link to several subunits of RNAP (Kulkarni & Summers 1999). In the absence of Hg(II) the ternary complex of MerR, RNAP, and merOP represses transcription of the mer structural genes. In the presence of mercuric ions, one Hg(II) per MerR homodimer (O’Halloran et al. 1989; Shewchuk et al. 1989a) coordinates in a trigonal manner to three essential cysteine residues of the MerR homodimer, two cysteines from one monomer, and one from the other (Helmann et al. 1990; Utschig et al. 1995). Hg(II) binding to the MerR homodimer results in both a relaxation of the DNA bends induced by apo-MerR, and both DNA distortion (Frantz &O’Halloran 1990) and an allosteric under winding of the promoter sequence by approximately 33(Ansari et al. 1992). The underwinding of the promoter DNA aligns the-10 and -35 sequences, such thatRNApolymerase can recognize and bind to these sites, initiating transcription from PmerT (Heltzel et al. 1990; Ansari et al.1992, 1995). The current model describes a resistance mechanism whose expression is repressed until Hg(II) ions enter the cytoplasm of the cell, but is primed to initiate transcription of the resistance genes at very low levels of Hg(II), and to fully induce resistance gene expression across a narrow increase in external Hg(II) concentration.

Reference:

Butler A. 1998 Acquisition and utilization of transition meal ions by marine organisms. Science 281, 207–210. Outten FW, Outten CE, O’Halloran TV. 2000a Metalloregulatory systems at the interface between bacterial metal homeostasis and resistance. In: Storz G, Hengge-Aronis R,(eds.) Bacterial stress responses. American Society of Microbiology;Washington DC: pp. 145–157. Outten FW, Outten CE, Hale J, O’Halloran TV. 2000b Transcriptional activation of an Escherichia coli copper efflux regulon by the chromosomal MerR homologue CueR. J Biol Chem 275, 31024–31029 Barkay T, Miller SM, Summers AO. 2003 Bacterial mercury resistance from atoms to ecosystems. FEMS Microbiol Rev 27, 355–384. Hobman JL, Brown NL. 1997 Bacterial mercury resistance genes. In: Sigel A, Sigel H, eds. Metal Ions in Biological Systems. New York: Marcel Dekker; 34, 527–568. Brown NL, Stoyanov JS, Kidd SP, Hobman JL. 2003 The MerR family of transcriptional regulators. FEMS Microbiol Rev 27, 145–163. O’Halloran TV, Frantz B, Shin MK, Ralston DM, Wright JG.1989 The MerR heavy metal receptor mediates positive activation in a topologically novel transcription complex.Cell 56, 119–129. Rouch DA, Parkhill J, Brown NL. 1995 Induction of bacterial mercury- and copper-responsive promoters: functional differences between inducible systems and implications for their use in gene-fusions for in vivo metal biosensors. J Ind Microbiol 14, 249–253. Shewchuk LM, Verdine GL, Walsh CT. 1989b Transcriptional switching by the metalloregulatory MerR protein: initial characterization of DNA and mercury (II) binding activities.Biochemistry 28, 2331–2339. Harley CB, Reynolds RP. 1987 Analysis of Escherichia coli promoter sequences. Nuc Acids Res 15, 2343–2361. Browning DF, Busby SJW. 2004 The regulation of bacterial transcription initiation. Nat Rev Microbiol 2, 57–65. Ansari AZ, Bradner JE, O’Halloran TV. 1995 DNA-bend modulation in a repressor-to-activator switching mechanism. Nature 374, 371–375. Kulkarni R, Summers AO. 1999 MerR crosslinks to the a, b and r70 subunits of RNA polymerase in the preinitiation complex at the merTPCAD promoter. Biochemistry 38, 3362–3368. Utschig LM, Bryson JW, O’Halloran TV. 1995 Mercury-199 NMR of the metal receptor site in MerR and its protein-DNA complex. Science 268, 380–385. Ansari AZ, Chael ML, O’Halloran TV. 1994 Allosteric under winding of DNA is a critical step in positive control of transcription by Hg-MerR. Nature 355, 87–89.

Parts

Quorum sensing system

Our part is constituted by Plac、luxR、Pluxr、luxr. LuxI can result in the production of AHL—a kind of auto-inducer which can ...

Fast-type merR biosensor

merR gene acts as a regulator which can promote downstream genes’ expression in the presence of Hg (II), the detailed mechanism of it is well-known as a whole...

Hg(II)
sensor

We built our part with an existed part----which provided us a PmerT promoter----and a merR sequence synthetized by ourselves.However, you need to add upstream an rbs to make it work if you decide to adopt our part....

K1513000: quorum sensing system

Our part is constituted by Plac、luxR、Pluxr、luxr. LuxI can result in the production of AHL—a kind of auto-inducer which can bind to the protein LuxR and induce the high expression of downstream genes of Pluxr，therefore，this system can drive a mass of bacteria highly express a particular gene. What’s more, AHL can be used by all bacteria in certain range.

K1513001: Fast-type merR biosensor

merR gene acts as a regulator which can promote downstream genes’ expression in the presence of Hg (II), the detailed mechanism of it is well-known as a whole. This summer we aimed at finding out a fast as well as accurate method to detect the Hg (II) in water. However, we found that the former method costs much time, so we turn to look for a rapid way which meets our expectation.

K1513002：Hg(II) sensor

We built our part with an existed part----which provided us a PmerT promoter----and a merR sequence synthetized by ourselves. However, you need to add upstream an rbs to make it work if you decide to adopt our part.

Saftey

Human Practice

Meet Up with BIT-iGEM

23rd Mar. 2014

The influence of temperature on the engineering bacteria in the fermentation tank

Synthetic Biology Symposium

24th Sep. 2014

2014 Metabolic Engineering and Synthetic Biology Symposium

Briefing Session in university

21st Sep. 2014

Introduction of the concept of Synthetic Biology and the meaning of iGEM

Project: The influence of temperature on the engineering bacteria in the fermentation tank

23rd Mar 2014 Meet Up with BIT-iGEM

Encountered Problems:

1、 too complicated gene pathway to accomplish in a short period of time;

2、 the effects of Web site, poster and other artists are not so beautiful;

3、 time for Sponsorship and advertising is a bit tight;

4、 visa and travel arrangements should be considered early.

Notice:

1、 DNA distribution must be tested before you intend to use one of those parts;

2、 Meet up between teams and parts interchange needed to be paid attention to;

3、 Souvenirs are recommended;

4、 Preparation of team T-shirt and mascot;

5、 Pamphlets are really necessary , portable, convenient and practical .

Help Issues:

1、 the bacterial genes and genetic circuits implementation;

2、 some help about experimental skills and techniques;

3、 can provide temperature sensitive strains if needed.

Project: Microfluidic technology application of antibiotics detection in the milk

3rd May 2014 Meet Up with BIT-China

Reason for losing the high award:

1、 for lack of time;

2、 for lack of experience;

3、 for lack of awards.

Notice:

1、 Pay close attention to official website;

2、 the subject should be in line with international ideas and the international market;

3、 Show every work during the event, and sum up experience.

Art design:

1、 logo、poster、wiki、pamphlets should share the same style, keeping the fluency and clarity of thought；

2、 pamphlets :for the use of information interchange; also can be a nice way to deepen mutual understanding between the teams;

3、 small gifts should also be considered and designed.

Domestic propaganda:

1、introduction of the synthetic biology, iGEM competition, and previous teams;

2、communication between teams and substantial help should be done.

Help Issues:

1、 to help us process a kind of chips of which the channel is 50μm;

2、 help to complete moulding and chips processing;

3、 to do some related experiment in exchange lab.

Project: Introduction of the concept of Synthetic Biology and the meaning of iGEM

21st Sep. 2014 Briefing Session in university

To make iGEM and Synthetic Biology be known to more people, especially the students who are major in biology and who intend to engage in biology in the coming future, BUCT-China came to Beijing University of Chemical Technology to deliver a report to the lower grades.

Before the report, we also took some other measures like designing our poster and delivering leaflets. Besides, A wide range of activities in social networks as MicroBlog and WeChat are organized.

In the keynote, six of our team members introduced the contents and the meaning of synthetic biology in turn; explain the iGEM concept and previous iGEM competitions, along with the Giant Jamboree. Also, we made necessary introduction of our own project, which was really of great popularity among the junior.

After the report, there were still some students interested in the competition and asked several questions like the ideas about synthetic biology as far as they were concerned and the information of how to join in the next year’s iGEM competition. Obviously they got known much more. Meanwhile, we’d got a lot ideas from the audience. Without hesitation, we’ve made it a success as far as the meaning and the adequate influence. Our team members also gained more confidence of ourselves.

Click to view the flyer

Click to view Beijing institute of technology invitation

Team

WEI WEI

Very cool friend, which is a song of life

I am responsible for chips、instrument and software.Own the characteristic

of being busy doing something. Darkness gives me the clear mind, therefore
I am used to staying up late.

ZHAO ZHANG

In me the tiger sniffs the rose.

Do not hesitate to become a
better one. Always keep the state
of fighting. Believe in
the feeling and hence live on.

QI SUN

The proof of the pudding

is in the eating

I can not discribe my character ,
but I know I am optimistic and
self-cofidence.Sometimes,I prefer to stay
alone, reading or writing.

HONGWEI DUAN

Most handsome boy in lab with

a gifted sense of humor.

To chase with a trembling body,
to challenge in awe.

DONGDONG LIU

Action speak louder than words
I like runing and reading.I am major in
biological engineering.I have a great
interest in biology.

JUANHONG GAO

Difficulty will be gone ,

everything will be OK!

I am a honest girl，
I like painting,running,playingtable
tennis.and my favourite thing is life ,
because I always believe life is nice.

TIAN ZHAO

Yesterday is history; Tomorrow

is mistery; Today is a gift,

that's why we call it present.

I am a junior major in bioengineering.
I think I'm quite outgoing and I have a
high sense of responsibility, also, I like
making friends.

YANNAN WANG

Don’t forget the beginner’s mind

then here comes the success.

I am a sunny boy.I enjoy the process
of each experiment .

SHENGWEI ZHU

Learn through playing.

So happy to see you guys. I'm a boy...
eh no...I'm a man who is full of
enthusiasm and loves life. I like seeking
for freedom and joy in daily life.

JIE HE

The length of life extremely is not
important, as long as you live happy,
live to do something meaningful,
it is enough.

RUOBING XU

There is no such thing as

darkness; only a failure to see

An outgoing girl who likes ACG,
reading and cooking very much.

HAO MI

Don't go too far and forget why.

SHENWANG CAO

I love BUCT-China.

WEI DAI

The golden age is before us,

not behind us.

SIRUI PAN

I like the dreams of the future

better than the history of the past.

Attributions

90%

Labblog

70%

Protocol

60%

Photo Gallery

40%

Judging

100%

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-<h1 >WELCOME TO iGEM 2014! </h1>
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-<a href="https://igem.org/Team.cgi?id=1513"> Official Team Profile </a></td>
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-<a href="https://2014.igem.org/Team:BUCT-China/Project"style="color:#000000"> Project</a></td>
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+		  		<h3>Project</h3>
+				<div class="col_1_of_3 span_1_of_3" style="margin-left:10px;">
+					<img src="https://static.igem.org/mediawiki/2014/d/d8/BUCT-CHINA-SERVICE1.png" alt="">
+					<h4><a href="">Overview</a></h4><p></p>
+					<p>There are a population of 884 million still drink water without the previous purification all over the world...</p>
+					<p><a class="popup-with-zoom-anim" href="https://2014.igem.org/Team:BUCT-China/small-dialogpj1">MORE</a></p><!--#small-dialogpj1-->
+				</div>
+				<div class="col_1_of_3 span_1_of_3">
+					<img src="https://static.igem.org/mediawiki/2014/5/5e/Service2.png" alt="">
+					<h4><a href="">Biosensors mining</a></h4>
+					<p>Quorum sensing was first found in Vibrio fischeri in the ocean. Microorganisms achieve information communication by ...</p>
+					<p><a class="popup-with-zoom-anim" href="https://2014.igem.org/Team:BUCT-China/small-dialogpj2">MORE</a></p>
+				</div>
+                <div class="col_1_of_3 span_1_of_3">
+				<img src="https://static.igem.org/mediawiki/2014/f/fe/Service3.png" alt="">
+					<h4><a href="">Biosensors</a></h4><p></p>
+					<p>Adoption of luminescent bacteria for air pollution monitoring, antibiotics screening and rapid...</p>
+					<p><a class="popup-with-zoom-anim" href="https://2014.igem.org/Team:BUCT-China/small-dialogpj4">MORE</a></p>
+				</div>
+				<div class="col_1_of_3 span_1_of_3">
+					<img src="https://static.igem.org/mediawiki/2014/7/7b/14.png" alt="">
+					<h4><a href="">Device</a></h4><p></p>
+					<p>This device includes two parts, one of them is physical instrument and the other is matched software...<BR></p><p></p>
+					<p><a class="popup-with-zoom-anim" href="https://2014.igem.org/Team:BUCT-China/small-dialogpj3">MORE</a></p>
+				</div>
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+						<div style="background-color:#FFF">
+							<div class="payment-online-form-left">
+                                 <h1 style="font-size:36px; top:20px; text-align: center;">overview</h1>
+							</div>
+                                  <hr>
+								<p></p>
+                                <dir>
+                                <p style="text-align:left">There are a population of 884 million still drink water without the previous purification all over the world. There are about 3 billion to 4 billion families lack of drinkable water. Each year about 3.5 million people's deaths are associated with insufficient water supply and sanitation condition, which occurs mainly in developing countries.
-<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+According to all above, the matter of water quality is a great matter of human beings’ life quality. It has always been crucial to many researchers engaging in improving and making breakthroughs on the approaches towards water quality detection. Currently chemical detection methods are more complex and time consuming; besides ,on the way of bringing the samples to the laboratory, some of the indicators may probably be changed. Another thing we may acknowledge is that chemical detection apparatus cost too much money.
-<a href="https://2014.igem.org/Team:BUCT-China/Parts"style="color:#000000"> Parts</a></td>
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+Based on the matters above, this year we intended to look for a new way using biotechnology. Here come the ideas, through two kinds of approaches we may achieve rapid water quality detection. One of the approaches is so called comprehensive detection, here we mainly rely on LuxAB ,which encodes a kind of luciferase. Because anything toxic within the water may have a negative impact on the activity of luciferase, the luminescence is likely to be decreased. The other is specific detection, because only in this way can we successfully quantify specific metal ions like Hg(II). One sequence of MerR operon occupies a significant position along with quorum sensing system. In addition, we use gfp as reporter.
-<a href="https://2014.igem.org/Team:BUCT-China/Modeling"style="color:#000000"> Modeling</a></td>
+Besides the two biosensors we built, the joint uses of microfluidic chips, automatic detection devices, photoelectric detection technology and micro total analysis systems will extremely promote biological detection method. This device can also be linked to the computer and has the ability of offline CNC screen operation, which improves the detection efficiency. What’s more, little device is convenient to carry and free from  limitation of experimental conditions.
+Biological method not only has the priority of concise operations and less consumption, but also has the advantages of high accuracy, specificity, and security.
+</p>
+                                </dir>
+                      </div>
+</div>
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-<a href="https://2014.igem.org/Team:BUCT-China/Notebook"style="color:#000000"> Notebook</a></td>
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+						<div style="background-color:#FFF">
+							<div class="payment-online-form-left">
+                                 <h1 style="font-size:36px; top:20px; text-align: center;">Biosensors Mining</h1>
+							</div>
+                                  <hr>
+								<p></p>
+                                <dir>
+                                <p style="text-align:left">Quorum sensing was first found in Vibrio fischeri in the ocean. Microorganisms achieve information communication by releasing a signaling molecules referred as auto inducer(AI). AI will promotes related gene expression in bacteria, meanwhile regulate microbes’ biological behaviors. Bacteria use quorum sensing to coordinate certain behaviors such as biofilm formation, virulence, bioluminescence and antibiotic resistance, based on the local density of bacteria. Thus microbes can collaborate to achieve cell populations’s engineering.
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+In Vibrio fischeri , plux promoter regulates the expression of quorum sensing system while plux promoter is automatically regulated by AI. PluxL and pluxR respectively promote transcription to the left and right. PluxL regulates transcription of LuxR gene ;in the meantime, pluxR can also start expression of luxI and fluorescence related genes. LuxI, is responsible for production of N-acyl-homoserine-lactone (AHL) autoinducer while luxR,is activated by this autoinducer to increase transcription of the luciferase operon.
-<a href="https://2014.igem.org/Team:BUCT-China/Safety"style=" color:#000000"> Safety </a></td>
+The enhanced E.coli quorum sensing system we reconstructed is greatly dependent to plac promoter. Plac will serve as pluxL in this system to promote transcription. Once the transcription has been started by plac, it will extend to series genes such as luxR、pluxR and luxI downstream. Noticeably, the LuxI/LuxR genes form a functional pair, considering LuxI as the auto-inducer synthase and LuxR as the receptor. We can obtain translation products luxI to catalyze the formation of AHL.
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+The yield of luxI protein is very low with a tenuous concentration of cells, likewise result in the subtle concentration of AHL in periplasmic. As the amount of cells increases, the density of AHL will reach a threshold value, this can strongly accelerate the formation of one kind of complex which is composed of AHL and luxR , also the complex will firmly promotes transcription of pluxR ,hence we acquire desired expression of bioluminescence related genes as well as increasing amount of luxI、luxR、AHL, which in turn form a positive regulation. In addition, plac is a fully functional part which act as a substitution to pluxL, preventing pluxL from inhibition of the AHL-LuxR complex, what’s more, releasing the negative influence on the expression of LuxR.
-<a href="https://2014.igem.org/Team:BUCT-China/Attributions"style="color:#000000"> Attributions </a></td>
+Based on this enhanced E.coli quorum sensing system, we got a innovative method for specific metal ions detection. The highlight is that we combine MerR and relevant promoter sequence pMerT to the downstream of luxI. The MerR family of metal-binding, metal-responsive proteins is unique in that they activate transcription from unusual promoters and coordinate metals through cysteine residues. They have conserved primary structures yet can effectively discriminate metals in vivo. Expression of MerR (in the absence of MerR and Hg (II) in the cell) proceeds from the PmerR promoter.
+In the absence of Hg(II), the MerR homodimer binds to the operator region within  the divergent  promoter  with binding centered on the dyad symmetrical DNA sequence between the -35 and -10 sequences of  PmerT, slightly repressing transcription of the structural gene promoter, and repressing transcription of merR from the PmerR promoter.
+Once the MerR homodimer has bound to merOP, recruitment of RNAP to the mer promoter occurs , and MerR has been shown to cross-link to several subunits of RNAP .
+In the presence of mercuric ions, one Hg(II) per MerR homodimer coordinates in a trigonal manner to three essential cysteine residues of the MerR homodimer, two cysteines from one monomer, and one from the other. Hg(II) binding to the MerR homodimer results in both a relaxation of the DNA bends induced by apo-MerR, and both DNA distortion and an allosteric under winding of the promoter sequence by approximately 33. The under winding of the promoter DNA aligns the -10 and -35 sequences, such that RNA polymerase can recognize and bind to these sites, initiating transcription from PmerT .
-<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
+The reporter gene we apply is gfp ,which also depends on the transcription of the same promoter pMerT.
-</tr>
+According to the design, we intended to make best use of the recombinant E.coli quorum sensing system to increase the bacteria density to a threshold value and produce a sufficient amount of AHL and LuxR protein, forming a continuous positive feedback. Meanwhile, there supposed to be a “switch” referred as MerR and pMerT. Only the presence of Hg(II) do drive the expression of structural genes and fluorescence related gene. Moreover, the fluorescence intensity should theoretically link a certain correlation to the concentration of Hg(II) in internal condition. For the purpose of continuous detection, what should not be ignored is that we desire enough MerR homodimer to bind to pMerT then interdict the transcription pathway. This clarifies the second aspect of recombinant E.coli quorum sensing system. Concluded, we designed this innovative system intending to get a fasten detection method towards specific metal ions in vivo.
-</table>
+</p>
+                                </dir>
+                      </div>
+</div>
+<!--  pj3-->
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+                                 <h1 style="font-size:36px; top:20px; text-align: center;">Project: The influence of temperature on the engineering bacteria in the fermentation tank</h1>
+							</div>
+                                  <img src="images/bl1.jpg" alt="" align="absmiddle" />
+                                  <hr>
+								<p></p>
+                                <h2 style="font-size:24px; left:20px;text-align:left"><b>23rd  Mar 2014   Meet Up with BIT-iGEM</b></h2><br>
+                                <dir>
+                                <p style="text-align:left"><b>Encountered Problems:</b></p>
+                                <p style="text-align:left">1、	too complicated gene pathway to accomplish in a short period of time;</p>
+                                <p style="text-align:left">2、	the effects of Web site, poster and other artists are not so beautiful;</p>
+                                <p style="text-align:left">3、	time for Sponsorship and advertising is a bit tight;</p>
+                                <p style="text-align:left">4、	visa and travel arrangements should be considered early.</p>
+                                <br>
+                                <p style="text-align:left"><b>Notice:</b></p>
+                                <p style="text-align:left">1、	DNA distribution must be tested before you intend to use one of those parts;</p>
+                                <p style="text-align:left">2、	Meet up between teams and parts interchange needed to be paid attention to;</p>
+                                <p style="text-align:left">3、	Souvenirs are recommended;</p>
+                                <p style="text-align:left">4、	Preparation of team T-shirt and mascot;</p>
+                                <p style="text-align:left">5、	Pamphlets are really necessary , portable, convenient and practical .</p>
+                                <br>
+                                <p style="text-align:left"><b>Help Issues:</b></p>
+                                <p style="text-align:left">1、	the bacterial genes and genetic circuits implementation;</p>
+                                <p style="text-align:left">2、	some help about experimental skills and techniques;</p>
+								<p style="text-align:left">3、	can provide temperature sensitive strains if needed.</p><br><br>
+                                </dir>
+                      </div>
+</div>
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+                                 <h1 style="font-size:36px; top:20px; text-align: center;">Sensors</h1>
+							</div>
+                                  <hr>
+								<p></p>
+                                <dir>
+                                <h2 style="font-size:30px; top:30px; text-align: center;">LuxAB---comprehensive biosensor</h1> <br><br>
+                                <p style="text-align:left"><b>Luminescent Bacteria Method：</b></p>
+                                <p style="text-align:left">Adoption of luminescent bacteria for air pollution monitoring, antibiotics screening and rapid detection of water attracts wide attention in recent years. Luminescent bacteria is a kind of bacteria that can produce visible fluorescence in normal conditions, while its bioluminescence is produced by molecular oxygen and catalyzed by luciferase inside the cell. Luciferase oxidates flavin mononucleotide (FMNH2) and long chain fatty aldehyde in the reduction state to FMN and long chain fatty acid, at the same time emits blue-green light in 450 ~ 490 nm. Equation can be expressed as follows:</p>
+                                <p style="text-align:left">2、	the effects of Web site, poster and other artists are not so beautiful;</p>
+                                <p style="text-align:left">The process of luminescence involves a variety of substances, which are products of bacterial metabolism. When external condition worsens, its luminous way will be affected in a very short period of time, resulting in suppression or shutdown of luminescent reaction. As a result, luminescence of bacteria is positively correlated to the concentration of toxin in condition, what’s more, the correlation can be approached by a linear function. Based on the mechanism, luminescent bacteria have been widely adopted as a biological indicator in rapid quantification of toxin in the environment. </p>
+                                 <br><br>
+                                <p style="text-align:left"><b>Luminescent bacteria luciferase gene </b></p>
+                                <p style="text-align:left">Bacteria luminescence is a consequence of its luciferase catalytic metabolic process. The luciferase encoding gene is LuxAB. All genus of luminescent bacteria have five core constitute lux gene operon, order of which is luxCDABE. Bacteria luciferase is composed of two subunits heterodimer, including luciferase alpha and beta subunits coded by luxA and luxB respectively. LuxC, luxD, luxE encode for reduction of fatty acid synthesis fatty aldehyde enzyme complexes of three subunits: reductase, transferase, synthetase, respectively. Mancin (1988) successfully cloned the photobacterium phosphoreum’s luciferase operon luxCDABFE and expressed it in Escherichia coli. Except for methods though plasmid, lux gene can also be integrated into the cell chromosome by genetic engineering techniques in pursuit of stability. What’s more, bioluminescent detection through lux gene is easy to achieve, and it is fast, simple and sensitive.
+Hence the bioluminescent detection method has extensive research and application in gene transduction, expression and regulation, environmental chemistry and its toxicity detection,etc.
+</p>
+                                 <br><br>
+                                <p style="text-align:left"><b>Reference:</b></p>
+                                <p style="text-align:left">Shi J, Frymier P D. Toxicity of metals and organic chemicals evaluated with
+bioluminescence assays[J]. Chemosphere, 2005, 58: 543-550.
+Ru CX, Yang SD. Luminescent bacteria lux report gene system evaluation and application [J]. Microbiology, 2000, 27(4):297-299.
+</p>
+                             <br><br> <br><br>
+                             <h2 style="font-size:30px; top:30px; text-align: center;">MerR ---Hg(II) Biosensor</h1>
+                                <p style="text-align:left">Metals can pose a particular problem to bacteria,because essential metals may be limiting in the environment, requiring active uptake mechanisms to import minimum concentrations of essential metals (Butler 1998; Outten et al. 2000).Bacteria can encounter purely toxic metals, such as Hg, Cd and As, which have no beneficial role in cellular metabolism, and must be avoided, removed or neutralized metal. The key first step in how bacteria respond to varying levels of both toxic and essential metals in the internal environment of the cell is due to the metal sensor and regulator proteins that they encode. There are several known types of prokaryotic metal ion sensing regulators. These include the MerR and Fur families of regulators, the ArsR/SmtB family repressors and several other structural regulator types .</p> <br><br>
+                               <p style="text-align:left"><b>MerR – a mercury sensing gene activator</b></p>
+                                <p style="text-align:left">A key class of prokaryotic metal ion responsive activators is the MerR family, of which the mercuric ion sensing MerR is the archetype. The closely-related 144 amino-acid MerR proteins from the mercuric ion resistance (mer) operons from transposons Tn501 and Tn21 have been the most heavily studied of these proteins (reviewed in Summers 1992, 1986; Hobman & Brown 1997;Outten et al. 2000a; Barkay et al. 2003; Brown et al.2003, and references therein), and the mechanism of Hg(II) resistance is now well known (reviewed most recently by Barkay et al. (2003)).
+In the mer operon from Tn501, MerR binds to operator DNA within a divergent promoter and regulates both its own expression and  expression of the downstream structural genes. The MerR family of metal-binding, metal-responsive proteins is unique in that they activate transcription from unusual promoters and coordinate metals through cysteine residues. They have conserved primary structures yet can effectively discriminate metals in vivo.
+Activation of transcription from the PmerT promoter by MerR in response to Hg(II) is hypersensitive (Ralston &O’Halloran 1990; Rouch et al. 1995), with virtually total induction of promoter activity occurring across a very narrow Hg(II) concentration rang. MerR has a strong selectivity for Hg(II) (Ralston & O’Halloran 1990)and Hg(II) has a very high affinity for MerR (Shewchuk et al. 1989b).
+</p><br><br>
+                                <p style="text-align:left"><b>The model for regulation of the mer promoter</b> </p>
+                               <p style="text-align:left"> The current model for MerR activation of transcription proposes that expression of MerR (in the
+absence of MerR and Hg (II) in the cell) proceeds from the PmerR promoter. In the absence of Hg(II), the MerR homodimer binds to the operator region within  the divergent  promoter  with binding centered on the dyad symmetrical DNA sequence between the -35 and -10 sequences of  PmerT, slightly repressing transcription of the structural gene promoter, and repressing transcription of merR from the PmerR promoter. This is probably due to MerR interfering with RNA polymerase (RNAP) binding, or open complex formation. PmerT is unusual, it has a 19 bp spacing between the -35 and -10 sites , rather than the 16–18 bp  spacing found in most prokaryotic promoters (Harley & Reynolds 1987). This makes PmerT suboptimal for RNAP recognition of, and binding to, the -35 and -10 sequences, preventing formation of the open complex and transcriptional activation (see Browning & Busby 2004). In the absence of Hg(II), the tight binding of the apo-MerR homodimer to PmerT causes a further bending of the promoter DNA to itself, making it even less ideal for RNAP binding (Ansari et al. 1995). Once the MerR homodimer has bound to merOP, recruitment of RNAP to the mer promoter occurs (Heltzel et al. 1990), and MerR has been shown to cross-link to several subunits of RNAP (Kulkarni & Summers 1999). In the absence of Hg(II) the ternary complex of MerR, RNAP, and merOP represses transcription of the mer structural genes. In the presence of mercuric ions, one Hg(II) per MerR homodimer (O’Halloran et al. 1989; Shewchuk et al. 1989a) coordinates in a trigonal manner to three essential cysteine residues of the MerR homodimer, two cysteines from one monomer, and one from the other (Helmann et al. 1990; Utschig et al. 1995). Hg(II) binding to the MerR homodimer results in both a relaxation of the DNA bends induced by apo-MerR, and both DNA distortion (Frantz &O’Halloran 1990) and an allosteric under winding of the promoter sequence by approximately 33(Ansari et al. 1992). The underwinding of the promoter DNA aligns the-10 and -35 sequences, such thatRNApolymerase can recognize and bind to these sites, initiating transcription from PmerT  (Heltzel et al. 1990; Ansari et al.1992, 1995).
-</tr>
+The current model describes a resistance mechanism whose expression is repressed until Hg(II) ions enter the cytoplasm of the cell, but is primed to initiate transcription of the resistance genes at very low levels of Hg(II), and to fully induce resistance gene expression across a narrow increase in external Hg(II) concentration.
+</p>
+                                <br><br>
+                                <p style="text-align:left"><b>Reference:</b></p>
+                                <p style="text-align:left">Butler A. 1998 Acquisition and utilization of transition meal ions by marine organisms. Science 281, 207–210.
+Outten FW, Outten CE, O’Halloran TV. 2000a Metalloregulatory systems at the interface between bacterial metal homeostasis and resistance. In:
+Storz G, Hengge-Aronis R,(eds.) Bacterial stress responses. American Society of Microbiology;Washington DC: pp. 145–157.
+Outten FW, Outten CE, Hale J, O’Halloran TV. 2000b Transcriptional activation of an Escherichia coli copper efflux regulon by the chromosomal MerR homologue CueR. J Biol Chem 275, 31024–31029
+Barkay T, Miller SM, Summers AO. 2003 Bacterial mercury resistance from atoms to ecosystems. FEMS Microbiol Rev 27, 355–384.
+Hobman JL, Brown NL. 1997 Bacterial mercury resistance genes. In: Sigel A, Sigel H, eds. Metal Ions in Biological Systems. New York: Marcel Dekker; 34, 527–568.
+Brown NL, Stoyanov JS, Kidd SP, Hobman JL. 2003 The MerR family of transcriptional regulators. FEMS Microbiol Rev 27, 145–163.
+O’Halloran TV, Frantz B, Shin MK, Ralston DM, Wright JG.1989 The MerR heavy metal receptor mediates positive activation in a topologically novel transcription complex.Cell 56, 119–129.
+Rouch DA, Parkhill J, Brown NL. 1995 Induction of bacterial mercury- and copper-responsive promoters: functional differences between inducible systems and implications for their use in gene-fusions for in vivo metal biosensors. J Ind Microbiol 14, 249–253.
+Shewchuk LM, Verdine GL, Walsh CT. 1989b Transcriptional switching by the metalloregulatory MerR protein: initial characterization of DNA and mercury (II) binding activities.Biochemistry 28, 2331–2339.
+Harley CB, Reynolds RP. 1987 Analysis of Escherichia coli promoter sequences. Nuc Acids Res 15, 2343–2361.
+Browning DF, Busby SJW. 2004 The regulation of bacterial transcription initiation. Nat Rev Microbiol 2, 57–65.
+Ansari AZ, Bradner JE, O’Halloran TV. 1995 DNA-bend modulation in a repressor-to-activator switching mechanism. Nature 374, 371–375.
+Kulkarni R, Summers AO. 1999 MerR crosslinks to the a, b and r70 subunits of RNA polymerase in the preinitiation complex at the merTPCAD promoter. Biochemistry 38, 3362–3368.
+Utschig LM, Bryson JW, O’Halloran TV. 1995 Mercury-199 NMR of the metal receptor site in MerR and its protein-DNA complex. Science 268, 380–385.
+Ansari AZ, Chael ML, O’Halloran TV. 1994 Allosteric under winding of DNA is a critical step in positive control of transcription by Hg-MerR. Nature 355, 87–89.
+</p>
+                             <br><br>
+                                </dir>
+                      </div>
+</div>
-</td>
-<tr> <td colspan="3"  height="15px"> </td></tr>
+				 <div class="clear"></div>
-<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
+   		   </div>
-<tr> <td colspan="3"  height="5px"> </td></tr>
+   		 </div>
+         <div>
+        <div class="pricing s5" id="pricing">
+					<div class="wrap">
+						<div class="pricing-head">
+							<h3>Parts</h3>
+							 <div style="margin-top:20px; width:100%; height:100px; text-align:center; color: #0FC;">
+                            </div>
+						</div>
+			   		 	<div class="pricing-grids">
+									<div class="pricing-grid1">
+									<h3><a><span><br>Quorum sensing system</span></a></h3>
+									<div>
+									  <P>Our part is constituted by Plac、luxR、Pluxr、luxr. LuxI can result in the production of AHL—a kind of auto-inducer which can ...</P>
+										  </div>
+									<div class="cart1">
+											<div class="span3"><a class="popup-with-zoom-anim" href="https://2014.igem.org/Team:BUCT-China/small-dialogpart1">
+											<j>MORE</j></a>
+										</div>
+										</div>
+									</div>
+									<div class="pricing-grid2">
+									  <h3><a><br>
+									    Fast-type merR biosensor</a></h3>
+										<!--<div class="price-value">
+											<a href="#">2简介</a>
+									  </div>-->
+										<div>
+                                        <p>merR gene acts as a regulator which can promote downstream genes’ expression in the presence of Hg (II), the detailed mechanism of it is well-known as a whole...</p>
+                                        </div>
+								  <div class="cart1">
+											<div class="span3"><a class="popup-with-zoom-anim" href="https://2014.igem.org/Team:BUCT-China/small-dialogpart2">
+											<k>MORE</k></a>
+										</div>
+										</div>
+									</div>
+									<div class="pricing-grid3">
+										<h3><a><span><br>
+										Hg(II)<br>
+sensor</span>
+							        <p></p></a></h3>
+										<!--<div class="price-value">
+											<a href="#">3简介</a>
+										</div>	-->
+										<div>
+                                        <p>We built our part with an existed part----which provided us a PmerT promoter----and a merR sequence synthetized by ourselves.However, you need to add upstream an rbs to make it  work if you decide to adopt our part.... </p>
+                                        </div>
+						  <div class="cart1">
+											<div class="span3"><a class="popup-with-zoom-anim" href="https://2014.igem.org/Team:BUCT-China/small-dialogpart3"><l>MORE</l></a>
+										</div>
+										</div>
+									</div>
+                                     <div style="margin-top:10px;width:100%;height:100px; text-align:center">
+                         <!--   <a class="wedobtn" href="#">先空着吧！或者写每月进度.</a>	-->
+                            </div>
+										<!-----pop-up-grid---->
+											 <div id="small-dialog" name="small-dialog" class="mfp-hide">
+												<div >
+												 	<div class="payment-online-form-left">
+                                                    <h1>K1513000: quorum sensing system</h1>
+                                                     <img src="https://static.igem.org/mediawiki/2014/a/ad/BUCT-CHINA-PART1.jpg" alt="" align="middle" />
+														<p>Our part is constituted by Plac、luxR、Pluxr、luxr. LuxI can result in the production of AHL—a kind of auto-inducer which can bind to the protein LuxR and induce the high expression of downstream genes of Pluxr，therefore，this system can drive a mass of bacteria highly express a particular gene. What’s more, AHL can be used by all bacteria in certain range.</p>
+													</div>
+									  			</div>
+											</div>
+											<!-----pop-up-grid---->
+                                            <!-----pop-up-grid---->
+											 <div id="small-dialog1" class="mfp-hide">
+												<div style="background-color:#FFF">
+												 	<div class="payment-online-form-left">
+                                                    <h1>K1513001: Fast-type merR biosensor</h1>
+                                                 <img src="https://static.igem.org/mediawiki/2014/f/fa/BUCT-CHINA-PART2.jpg" alt="" align="middle" />
+														<p>merR gene acts as a regulator which can promote downstream genes’ expression in the presence of Hg (II), the detailed mechanism of it is well-known as a whole. This summer we aimed at finding out a fast as well as accurate method to detect the Hg (II) in water. However, we found that the former method costs much time, so we turn to look for a rapid way which meets our expectation.</p>
+													</div>
+											</div>
+                                            </div>
+											<!-----pop-up-grid复制这个添加其他的弹出框---->
+                                             <div id="small-dialog2" name="small-dialog2" class="mfp-hide">
+												<div style="background-color:#FFF">
+												 	<div class="payment-online-form-left">
+                                                    <h1>K1513002：Hg(II) sensor</h1>
+                                                     <img src="https://static.igem.org/mediawiki/2014/a/a1/BUCT-CHINA-PART3.jpg" alt="" align="middle" />
+														<p>We built our part with an existed part----which provided us a PmerT promoter----and a merR sequence synthetized by ourselves. However, you need to add upstream an rbs to make it work if you decide to adopt our part.</p>
+													</div>
+											</div>
+										</div>
+						<div class="clear"> </div>
+   		            </div>
+				</div>
+				</div>
+        </div>
+<!--         这是modeling-->
+          <div></div>
+<!----safety---->
+   		 <div class="portfoliO s1" id="work"><!-- start portfoli1 -->
+   		 <div class="wrap">
+		 <h3>Saftey		 </h3>
+		 <p>&nbsp;</p>
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+								</div>
+</div>
+							<div class="portfolio card mix_all" data-cat="card" style="display: inline-block; opacity: 1;">
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+                        </div>
+			</div><!-- end container -->
+		</div>
+  <!----start-team-members---->
+				<div class="team-members s2" id="about">
+					<div class="wrap">
+						<div class="tm-head">
+							<h3>Human Practice</h3>
+<br>
+						</div>
+						<div class="tm-head-grids">
+                      <!----第一次交流---->
+							<div class="tm-head-grid">
+								<img src="https://static.igem.org/mediawiki/2014/6/67/Blt1.jpg" alt="" />
+								<h4>Meet Up with BIT-iGEM</h4>
+								<h5>23rd  Mar.  2014</h5><hr>
-<!--requirements section -->
+                              <p>The influence of temperature on the engineering  bacteria in the fermentation tank</p>
-<tr><td colspan="3"> <h3> Requirements </h3></td></tr>
+                                <!----read---->
-<tr>
+                  <div class="cart1">
-<td width="45%"  valign="top">
+									<div class="span3"><a class="popup-with-zoom-anim" href="https://static.igem.org/mediawiki/2014/e/e0/BIT-iGEM.pdf">
+										<k>READ   </k></a>
+									</div>
+							  </div>
+							</div>
+							<!----第四次交流---->
+							<div class="tm-head-grid">
+								<img src="https://static.igem.org/mediawiki/2014/9/9b/%E5%9B%BD%E9%99%85%E8%AE%BA%E5%9D%9B.jpg" alt="" />
+								<h4>Synthetic Biology Symposium</h4>
+							  <h5>24th Sep. 2014</h5><hr>
-<p> Please be sure to keep these links, your audience will want to find your: </p>
+                                <p>2014 Metabolic Engineering and Synthetic Biology Symposium </p>
+                                <div class="cart1">
+								<div class="span3"><a class="popup-with-zoom-anim" href="https://static.igem.org/mediawiki/2014/c/c3/2014_Metabolic_Engineering_and_Synthetic_Biology_Symposium.pdf">
+										<k>READ</k></a>
+								  </div>
+								</div>
+							</div>
+                               <!----第三次交流---->
+							<div class="tm-head-grid">
+								<img src="https://static.igem.org/mediawiki/2014/f/f0/Bht1.jpg" alt="" />
+								<h4>Briefing Session in university</h4>
+							  <h5>21st Sep. 2014</h5><hr>
-<!-- Links to other team pages -->
+                                <p>Introduction of the concept of Synthetic Biology and the meaning of  iGEM</p>
-<ul>
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-<li><a href="https://2014.igem.org/Team:BUCT-China">Home</a> </li>
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+										<k>READ</k></a>
-<li><a href="https://igem.org/Team.cgi?year=2013&team_name=BUCT-China">Official Team Profile</a> </li>
+								  </div>
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+							<div class="clear"> </div>
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+						</div>
-<li><a href="https://2014.igem.org/Team:BUCT-China/Attributions">Attributions</a> </li>
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+                    <!--北理1-->
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+                                 <h1 style="font-size:36px; top:20px; text-align: center;">Project: The influence of temperature on the engineering bacteria in the fermentation tank</h1>
+							</div>
+                                  <img src="https://static.igem.org/mediawiki/2014/c/cb/Bl1.jpg" alt="" align="absmiddle" />
+                                  <hr>
+								<p></p>
+                                <h2 style="font-size:24px; left:20px;text-align:left"><b>23rd  Mar 2014   Meet Up with BIT-iGEM</b></h2><br>
+                                <dir>
+                                <p style="text-align:left"><b>Encountered Problems:</b></p>
+                                <p style="text-align:left">1、	too complicated gene pathway to accomplish in a short period of time;</p>
+                                <p style="text-align:left">2、	the effects of Web site, poster and other artists are not so beautiful;</p>
+                                <p style="text-align:left">3、	time for Sponsorship and advertising is a bit tight;</p>
+                                <p style="text-align:left">4、	visa and travel arrangements should be considered early.</p>
+                                <br>
+                                <p style="text-align:left"><b>Notice:</b></p>
+                                <p style="text-align:left">1、	DNA distribution must be tested before you intend to use one of those parts;</p>
+                                <p style="text-align:left">2、	Meet up between teams and parts interchange needed to be paid attention to;</p>
+                                <p style="text-align:left">3、	Souvenirs are recommended;</p>
+                                <p style="text-align:left">4、	Preparation of team T-shirt and mascot;</p>
+                                <p style="text-align:left">5、	Pamphlets are really necessary , portable, convenient and practical .</p>
+                                <br>
+                                <p style="text-align:left"><b>Help Issues:</b></p>
+                                <p style="text-align:left">1、	the bacterial genes and genetic circuits implementation;</p>
+                                <p style="text-align:left">2、	some help about experimental skills and techniques;</p>
+								<p style="text-align:left">3、	can provide temperature sensitive strains if needed.</p><br><br>
+                                </dir>
+                      </div>
+</div>
+<!--北理2-->
+                        <div id="small-dialogbl2" name="small-dialogbl2" class="mfp-hide">
+						<div style="background-color:#FFF">
+							<div class="payment-online-form-left">
+                                 <h1 style="font-size:36px; top:20px; text-align: center;">Project: Microfluidic technology application of antibiotics detection in the milk</h1>
+							</div>
+                                  <img src="https://static.igem.org/mediawiki/2014/b/bf/Bl2.jpg" alt="" align="absmiddle" />
+                                  <hr>
+								<p></p>
+                                <h2 style="font-size:24px; left:20px;text-align:left"><b>3rd May 2014  Meet Up with BIT-China</b></h2><br>
+                                <dir>
+                                <p style="text-align:left"><b>Reason for losing the high award:</b></p>
+                                <p style="text-align:left">1、	for lack of time;</p>
+                                <p style="text-align:left">2、	for lack of experience;</p>
+                                <p style="text-align:left">3、	for lack of awards.</p>
+                                <br>
+                                <p style="text-align:left"><b>Notice:</b></p>
+                                <p style="text-align:left">1、	Pay close attention to official website;</p>
+                                <p style="text-align:left">2、	the subject should be in line with international ideas and the international market;</p>
+                                <p style="text-align:left">3、	Show every work during the event, and sum up experience.</p>
+                                 <br>
+                                <p style="text-align:left"><b>Art design:</b></p>
+                                <p style="text-align:left">1、	logo、poster、wiki、pamphlets should share the same style, keeping the fluency and clarity of thought；</p>
+                                <p style="text-align:left">2、	pamphlets :for the use of information interchange; also can be a nice way to deepen mutual understanding between the teams;</p>
+								<p style="text-align:left">3、	small gifts should also be considered and designed.</p>
+								 <br>
+                                <p style="text-align:left"><b>Domestic propaganda:</b></p>
+                                <p style="text-align:left">1、introduction of the synthetic biology, iGEM competition, and previous teams;</p>
+                                <p style="text-align:left">2、communication between teams and substantial help should be done.</p>
+                                <br>
+                                <p style="text-align:left"><b>Help Issues:</b></p>
+                                <p style="text-align:left">1、	to help us process a kind of chips of which the channel is 50μm;</p>
+                                <p style="text-align:left">2、	help to complete moulding and chips processing;</p>
+								<p style="text-align:left">3、	to do some related experiment in exchange lab.</p><br><br>
+                                </dir>
+                         </div>
+                      </div>
+                        <!--北化1-->
+                        <div id="small-dialogbh1" name="small-dialogbh1" class="mfp-hide">
+						<div style="background-color:#FFF">
+							<div class="payment-online-form-left">
+                                 <h1 style="font-size:36px; top:20px; text-align: center;">Project: Introduction of the concept of Synthetic Biology and the meaning of  iGEM</h1>
+							</div>
+                                  <img src="https://static.igem.org/mediawiki/2014/0/01/Bh1t.jpg" alt="" align="absmiddle" />
+                                  <hr>
+								<p></p>
+                                <h2 style="font-size:24px; left:20px;text-align:left"><b>21st Sep. 2014 Briefing Session in university
+</b></h2><br>
+                                <dir>
+                               <p style="text-align:left">To make iGEM and Synthetic Biology be known to more people, especially the students who are major in biology and who intend to engage in biology in the coming future, BUCT-China came to Beijing University of Chemical Technology to deliver a report to the lower grades.</p>
+                                <img src="https://static.igem.org/mediawiki/2014/8/82/Bh1.jpg" align="absmiddle"/>
+                                <br>
+                                <p style="text-align:left">Before the report, we also took some other measures like designing our poster and delivering leaflets. Besides, A wide range of activities in social networks as MicroBlog and WeChat are organized. </p>
+                                <img src="https://static.igem.org/mediawiki/2014/9/99/Bh2.jpg" align="absmiddle"/>
+                                 <br>
+                                <p style="text-align:left">In the keynote, six of our team members introduced the contents and the meaning of synthetic biology in turn; explain the iGEM concept and previous iGEM competitions, along with the Giant Jamboree. Also, we made necessary introduction of our own project, which was really of great popularity among the junior. </p>
+                                <img src="https://static.igem.org/mediawiki/2014/6/69/Bh3.jpg" align="absmiddle"/>
+								 <br>
+                                <p style="text-align:left">After the report, there were still some students interested in the competition and asked several  questions like the ideas about synthetic biology as far as they were concerned and the information of how to join in  the next year’s iGEM competition. Obviously they got known much more. Meanwhile, we’d got a lot ideas from the audience. Without hesitation, we’ve made it a success as far as the meaning and the adequate influence. Our team members also gained more confidence of ourselves. </p>
+                                <img src="https://static.igem.org/mediawiki/2014/7/7f/Bh4.jpg" align="absmiddle"/>
+                                  <img src="https://static.igem.org/mediawiki/2014/a/a6/Bh5.jpg" align="absmiddle"/>
+                                <br><br><p> </p>
+                                <p><a href="https://static.igem.org/mediawiki/2014/5/56/Xuanchuan1.pdf">Click to view the flyer</a></p>
+                                 <p><a href="https://static.igem.org/mediawiki/2014/1/19/Yaoqinghan.pdf">Click to view Beijing institute of technology invitation</a></p><br><br>
+                                </dir>
+                         </div>
+                      </div>
+ </div>
+                <!----//End-H-P---->
+<!---- 这是队伍成员 ---->
+				<div class="fun" id="team">
+					<div class="wrap">
+						<div class="fun-head">
+							<h3>Team</h3>
+<br>
+						</div>
+						<div class="fun-head-grids">
+							<div class="fun-head-grid">
+						    <img src="https://static.igem.org/mediawiki/2014/a/a2/WW.jpg" height="175px"/>
+                            <h5>WEI WEI</h5>
+			        			          <h6>Very cool friend, which is a song of life</h6>
+<h6>I am responsible for chips、instrument and software.Own the characteristic </h6>
+<h8>of being busy doing something. Darkness gives me the clear mind, therefore<br> I am used to staying up late.</h8>
+</div>
+<br>
+                          <div class="fun-head-grid">
+								<img src="https://static.igem.org/mediawiki/2014/6/6b/ZZ.jpg" height="175px"/>
+                            <h5>ZHAO ZHANG</h5>
+							<h6>In me the tiger sniffs the rose.</h6>
+								<h8>Do not hesitate to become a </h8><br>
+								<h8>better one. Always keep the state</h8><br>
+								<h8> of fighting. Believe in </h8><br>
+<h8>the feeling and hence live on.</h8>
+						  </div>
+							<div class="fun-head-grid">
+								<img src="https://static.igem.org/mediawiki/2014/9/9f/SQ.jpg" height="175px"/>
+                                                            <h5>QI SUN</h5>
+							  <h6>The&nbsp;proof&nbsp;of&nbsp;the&nbsp;pudding&nbsp;</h6>
+							  <h6>is&nbsp;in&nbsp;the&nbsp;eating</h6>
+                              <h8>I can not discribe my character ,</h8><br>
+                                        <h8>but I know I am optimistic and </h8><br>
+                                        <h8>self-cofidence.Sometimes,I prefer to stay </h8><br>
+                                        <h8>alone, reading or writing.</h8>
+                          </div>
+							<div class="fun-head-grid">
+						    <img src="https://static.igem.org/mediawiki/2014/6/6b/DU.jpg" height="175px"/>
+			          <h5>HONGWEI DUAN</h5>
+								<h6>Most handsome boy in lab with </h6>
+							  <h6>a gifted sense of humor.</h6>
+                                <H8>To chase with a trembling body, </H8><br>
+                                <H8>to challenge in awe.</H8>	<br>
+					      </div>
+							<div class="fun-head-grid">
+								<img src="https://static.igem.org/mediawiki/2014/0/05/LDD.jpg" height="175px"/>
+						    <h5>DONGDONG LIU</h5>
+								<h6>Action speak louder than words</h8>	<br>
+                                <h8>I like runing and reading.I am major in</h8><br>
+                                <h8> biological engineering.I have a great</h8><br>
+                                <h8> interest in biology.</h8>
+                          </div>
+                                	<div class="fun-head-grid">
+	<img src="https://static.igem.org/mediawiki/2014/4/41/GJ.jpg" height="175px"/>
+						    <h5>JUANHONG GAO</h5>
+						  <h6>Difficulty will be gone ,</h6>
+						  <h6>everything will be OK!</h6>
+<h8>I am a honest girl，</h8><br>
+<h8>I like painting,running,playingtable</h8><br>
+<h8> tennis.and my favourite thing is life ,</h8><br>
+<h8>because I always believe life is nice.</h8>
+</div>
+                            							<div class="fun-head-grid">
+								<img src="https://static.igem.org/mediawiki/2014/6/6e/ZT.jpg" height="175px"/>
+						    <h5>TIAN ZHAO</h5>
+																<h6>Yesterday is history; Tomorrow </h6>
+								<h6>is mistery; Today is a gift, </h6>
+								<h6>that's why we call it present.</h6>
+<h8>I am a junior major in bioengineering.</h8><br>
+<h8> I think I'm quite outgoing and I have a </h8><br>
+<h8>high sense of responsibility, also, I like</h8><br>
+<h8> making friends.</h8>
+                          </div>
+						  <div class="fun-head-grid">
+								<img src="https://static.igem.org/mediawiki/2014/5/58/WY.jpg" height="175px"/>
+					    <h5>YANNAN WANG</h5>
+								<h6>Don’t forget the beginner’s mind </h6>
+								<h6>then here comes the success.</h6>
+<h8>I am a sunny boy.I enjoy the process </h8><br>
+                                <h8>of each experiment . </h8>
+                          </div>
+                                                    <div class="fun-head-grid">
+								<img src="https://static.igem.org/mediawiki/2014/e/e0/ZS.jpg" height="175px"/>
+						    <h5>SHENGWEI ZHU</h5>
+							<h6>Learn through playing. </h6>
+                                <h8>So happy to see you guys. I'm a boy...</h8><br>
+                                <h8>eh no...I'm a man who is full of</h8><br>
+                                <h8>enthusiasm and loves life. I like seeking</h8><br>
+                                <h8> for freedom and joy in daily life. </h8>
+                          </div>
+							    <div class="fun-head-grid">
+								<img src="https://static.igem.org/mediawiki/2014/c/c0/HJ.jpg" height="175px"/>
+						    <h5>JIE HE</h5>
+								<h6>&nbsp;</h6>
+<h8>The  length of life extremely is not </h8><br>
+<h8>important, as long as you live happy, </h8><br>
+<h8>live to  do something meaningful,</h8><br>
+<h8> it is enough.</h8>
+                          </div>
+									      <div class="fun-head-grid">
+								<img src="https://static.igem.org/mediawiki/2014/8/8d/XR.jpg" height="175px"/>
+				    <h5>RUOBING XU</h5>
+								<h6>There is no such thing as</h6>
+								<h6> darkness; only a failure to see</h6>
+<h8>An outgoing girl who likes ACG, </h8><br>
+                            <h8>reading and cooking very much.</h8>
+                          </div>
+			      <div class="fun-head-grid">
+								<img src="https://static.igem.org/mediawiki/2014/0/05/MIH.jpg" height="175px"/>
+				    <h5>HAO MI</h5>
+								<h6>Don't go too far and forget why.</h6>
+                          </div>
+			      <div class="fun-head-grid">
+								<img src="https://static.igem.org/mediawiki/2014/a/a6/CSW.jpg" height="175px"/>
+				    <h5>SHENWANG CAO</h5>
+								<h6>I love BUCT-China. </h6>
+                          </div>
+			      <div class="fun-head-grid">
+								<img src="https://static.igem.org/mediawiki/2014/e/e2/DW.jpg" height="175px"/>
+				    <h5>WEI DAI</h5>
+								<h6>The golden age is before us,</h6>
+								<h6>not behind us.</h6>
+                          </div>
+			      <div class="fun-head-grid">
+								<img src="https://static.igem.org/mediawiki/2014/1/11/PSR.jpg" height="175px"/>
+				    <h5>SIRUI PAN</h5>
+								<h6>I like the dreams of the future </h6>
+								<h6>better than the history of the past.</h6>
+                          </div>
+						  <div class="clear"> </div>
+						</div>
+					</div>
+				</div>
+				<!----//End-TEAM---->
+   		        <!----start-notbook---->
+               <div class="slider_nb s7" id="notebook">
+					<div class="wrap">
+						<div class="ex-head">
+							<h3></h3>
+						</div>
+<div><p><br></p></div><div><p><br></p></div><div><p><br></p></div><div><p><br></p></div>
+						<div class="prog">
+								<div class="text">
+									<span><a href="https://static.igem.org/mediawiki/2014/d/d5/Attributions.pdf">Attributions</a></span>
+								</div>
+								<div class="text_p">
+									<span style="color:#333">90%</span>
+								</div>
+								<div class="clear"></div>
+								<div class="progress-bar-container" data-percent="90%">
+									<div class="progress-bar" style="width: 90%;">
+									</div>
+								</div>
+							</div>
+<div><p><br></p></div>
+							<div class="prog1">
+								<div class="text">
+									<span><a href="https://static.igem.org/mediawiki/2014/3/3c/BUCT-CHINA-LABBLOG.pdf">Labblog</a></h4><p></span>
+								</div>
+								<div class="text_p">
+									<span style="color:#333">70%</span>
+								</div>
+								<div class="clear"></div>
+								<div class="progress-bar-container" data-percent="90%">
+									<div class="progress-bar1" style="width: 70%;">
+									</div>
+								</div>
+							</div>
+<div><p><br></p></div>
+							<div class="prog2">
+								<div class="text" style="color:#333">
+									<span style="color:#333"><a href="https://static.igem.org/mediawiki/2014/b/bc/BUCT-CHINA-PROTOCOL.pdf">Protocol</a></span>
+								</div>
+								<div class="text_p">
+									<span style="color:#333">60%</span>
+								</div>
+<div class="clear"></div>
+								<div class="progress-bar-container" data-percent="90%">
+									<div class="progress-bar1" style="width: 60%; background-color:#D3D3D3">
+									</div>	</div>
+							</div>
+<div><p><br></p></div>
+<div class="prog2">
+								<div class="text">
+									<span><a href="https://static.igem.org/mediawiki/2014/2/2d/Photo_Gallery.pdf">Photo Gallery</a></span>
+								</div>
+								<div class="text_p">
+									<span style="color:#333">40%</span>
+								</div>
+								<div class="clear"></div>
+								<div class="progress-bar-container" data-percent="90%">
+									<div class="progress-bar2" style="width: 40%;">
+									</div>
+								</div>
+							</div><div><p><br></p></div>
+<div class="prog2">
+								<div class="text">
+									<span><a href="https://static.igem.org/mediawiki/2014/d/d2/Judging.pdf">Judging</a></span>
+								</div>
+								<div class="text_p">
+									<span style="color:#333">100%</span>
+								</div>
+								<div class="clear"></div>
+								<div class="progress-bar-container" data-percent="90%">
+									<div class="progress-bar2" style="width: 100%;background-color:#00FFFF">
+									</div>
+								</div>
+							</div>
+					</div>
+		</div>
+<!--               <div text-align:center;>
+                        <h3>&nbsp;</h3>
+							<h3>NOTBOOK</h3>
+							<p>&nbsp;</p>
+                            <h3>&nbsp;</h3>
+							<h3>NOTBOOK</h3>
+							<p>&nbsp;</p>
+                 </div>
+--></div>
+	<!-----cntact---->
+ <script>
+					 function addText_Btn()
+					{
+					 var targetDiv = document.getElementById("target");
+					 var srcText = document.getElementById("src");
+					 targetDiv.innerHTML = targetDiv.innerHTML+srcText.value+"<br>";
+					}
+				</script>
-</ul>
-</td>
+ <!-- scroll_top_btn -->
+		<script type="text/javascript" src="https://2014.igem.org/Wiki/buct/move-top.js?action=raw"></script>
+		<script type="text/javascript" src="https://2014.igem.org/Wiki/buct/easing.js?action=raw"></script>
+		<script type="text/javascript">
+		jQuery(document).ready(function($) {
+			$(".scroll").click(function(event){
+				event.preventDefault();
+				$('html,body').animate({scrollTop:$(this.hash).offset().top},1200);
+			});
+		});
+	</script>
-<td > </td>
+		 <a href="#" id="toTop" style="display: block;"><span id="toTopHover" style="opacity: 1;"></span></a>
-<td width="45%">
+<div style="display:none"><script src='http://v7.cnzz.com/stat.php?id=155540&web_id=155540' language='JavaScript' charset='gb2312'></script></div>
-<p>There are a few wiki requirements teams must follow:</p>
-<ul>
-<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
-<li>All pages must be created under the team’s name space.</li>
-<li>As part of your documentation, keep the links from the menu to the left. </li>
-<li>Do not use flash in wiki code. </li>
- <li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
-</ul>
-<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
-</td>
+<script type="text/javascript" src="https://2014.igem.org/Wiki/buct/jquery.mixitup.min.js?action=raw"></script>
-</tr>
+					<script type="text/javascript">
+						$(function () {
+							var filterList = {
+								init: function () {
+									// MixItUp plugin
+									// http://mixitup.io
+									$('#portfoliolist').mixitup({
+										targetSelector: '.portfolio',
+										filterSelector: '.filter',
+										effects: ['fade'],
+										easing: 'snap',
+										// call the hover effect
+										onMixEnd: filterList.hoverEffect()
+									});
+								},
+								hoverEffect: function () {
+									// Simple parallax effect
+									$('#portfoliolist .portfolio').hover(
+										function () {
+											$(this).find('.label').stop().animate({bottom: 0}, 200, 'easeOutQuad');
+											$(this).find('img').stop().animate({top: -30}, 500, 'easeOutQuad');
+										},
+										function () {
+											$(this).find('.label').stop().animate({bottom: -40}, 200, 'easeInQuad');
+											$(this).find('img').stop().animate({top: 0}, 300, 'easeOutQuad');
+										}
+									);
+								}
+							};
+							// Run the show!
+							filterList.init();
+						});
+					</script>
+<div>
-<tr> <td colspan="3"  height="15px"> </td></tr>
-<tr><td bgColor="#e7e7e7" colspan="3" height="1"> </tr>
-<!--tips  -->
+			</div>
-<tr><td colspan="3" > <h3> Tips  </h3></td></tr>
+  <div style="background-color:#FCF"; class="clients" id="sponsors">
+					<div class="wrap">
+						<div class="client-head">
+							<h3>Sponsors</h3>
+						</div>
+						<!---strat-image-cursuals---->
+		                  <div class="t-clients">
+		                  	<div class="wrap">
+									<div class="nbs-flexisel-container"><div class="nbs-flexisel-inner">
-<tr>
+									<ul class=" nbs-flexisel-ul" >
-<td width="45%" valign="top">
+<li onClick="location.href='http://www.buct.edu.cn';" class="nbs-flexisel-item" ><img src="https://static.igem.org/mediawiki/2014/a/a5/Bhlogo.png"></li>
-<p>We are currently working on providing teams with some easy to use design templates.
-<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
-<ul>
+<li onClick="location.href='http://www.bhphoton.com';" class="nbs-flexisel-item" ><img src="https://static.igem.org/mediawiki/2014/9/94/Binsong%281%29.png"></li>
-<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
-<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
-<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
-<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
-<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
-</ul>
-<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
+<li onClick="location.href='http://www.ibp.cas.cn';" class="nbs-flexisel-item" ><img src="https://static.igem.org/mediawiki/2014/8/81/Swwl.png"></li>
-</td>
-<td> </td>
+<li onClick="location.href='http://www.sibet.ac.cn';" class="nbs-flexisel-item" ><img src="https://static.igem.org/mediawiki/2014/a/ae/Swyx.jpg"></li>
-<td width="45%">
-<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+</ul></div></div> <hr><hr>
+           <div class="bottom-footer-left">
+                       <hr><hr>
+							<p style="font-size:20px"><span>Beijing University of Chemical Technology|15th North Third Ring East Road,Chaoyang District,Beijing|100029</span><br>shwww0512@163.com <br> Beijing University of Chemical Technology &copy;<a target="_blank" href="http://www.buct.edu.cn/">BUCT-China</a></p>
+	</div>
+									<div class="clear"> </div>
+								  <!---strat-image-cursuals---->
+										<script type="text/javascript" src="https://2014.igem.org/Wiki/buct/jquery.flexisel.js?action=raw"></script>
+										<!---End-image-cursuals---->
+										<script type="text/javascript">
+										$(window).load(function() {
+										    $(".flexiselDemo3").flexisel({
+										        visibleItems: 4,
+										        animationSpeed: 1000,
+										        autoPlay: true,
+										        autoPlaySpeed: 3000,
+										        pauseOnHover: true,
+										        enableResponsiveBreakpoints: true,
+										        responsiveBreakpoints: {
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+										                changePoint:480,
+										                visibleItems: 1
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+										                changePoint:640,
+										                visibleItems: 2
+										            },
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+										                changePoint:768,
+										                visibleItems: 3
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+										});
+										</script>
+								<!---->
+						  		<!---->
+						 	 </div>
+						</div>
+					</div>
+				</div>
-<ul>
+<script>
-<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+					$(document).ready(function() {
-<li>Be clear about what you are doing and what you plan to do.</li>
+						$('.popup-with-zoom-anim').magnificPopup({
-<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+							type: 'inline',
-<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
+							fixedContentPos: false,
-<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
+							fixedBgPos: true,
-<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
+							overflowY: 'auto',
-<li>Have lots of fun! </li>
+							closeBtnInside: true,
-</ul>
+							preloader: false,
-</br>
+							midClick: true,
-</td>
+							removalDelay: 300,
-</tr>
+							mainClass: 'my-mfp-zoom-in'
-</table>
+					});
+				});
+				</script>
+</body>
+</html>

Team:BUCT-China

From 2014.igem.org

Latest revision as of 03:20, 18 October 2014

Project

Overview

Biosensors mining

Biosensors

Device

overview

Biosensors Mining

Project: The influence of temperature on the engineering bacteria in the fermentation tank

23rd Mar 2014 Meet Up with BIT-iGEM

Sensors

LuxAB---comprehensive biosensor

MerR ---Hg(II) Biosensor

Parts

Quorum sensing system

Fast-type merR biosensor

Hg(II) sensor

K1513000: quorum sensing system

K1513001: Fast-type merR biosensor

K1513002：Hg(II) sensor

Saftey

Human Practice

Meet Up with BIT-iGEM

23rd Mar. 2014

Synthetic Biology Symposium

24th Sep. 2014

Briefing Session in university

21st Sep. 2014

Project: The influence of temperature on the engineering bacteria in the fermentation tank

23rd Mar 2014 Meet Up with BIT-iGEM

Project: Microfluidic technology application of antibiotics detection in the milk

3rd May 2014 Meet Up with BIT-China

Project: Introduction of the concept of Synthetic Biology and the meaning of iGEM

21st Sep. 2014 Briefing Session in university

Team

WEI WEI

Very cool friend, which is a song of life

I am responsible for chips、instrument and software.Own the characteristic

ZHAO ZHANG

In me the tiger sniffs the rose.

QI SUN

The proof of the pudding

is in the eating

HONGWEI DUAN

Most handsome boy in lab with

a gifted sense of humor.

DONGDONG LIU

Action speak louder than words I like runing and reading.I am major in biological engineering.I have a great interest in biology.

JUANHONG GAO

Difficulty will be gone ,

everything will be OK!

TIAN ZHAO

Yesterday is history; Tomorrow

is mistery; Today is a gift,

that's why we call it present.

YANNAN WANG

Don’t forget the beginner’s mind

then here comes the success.

SHENGWEI ZHU

Learn through playing.

JIE HE

RUOBING XU

There is no such thing as

darkness; only a failure to see

HAO MI

Don't go too far and forget why.

SHENWANG CAO

I love BUCT-China.

WEI DAI

The golden age is before us,

not behind us.

SIRUI PAN

I like the dreams of the future

better than the history of the past.

Sponsors

Hg(II)
sensor

Action speak louder than words
I like runing and reading.I am major in
biological engineering.I have a great
interest in biology.