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Team:TU Eindhoven/Notebook
From 2014.igem.org
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<li class="category"><a href="#september">SEPTEMBER</a></li> | <li class="category"><a href="#september">SEPTEMBER</a></li> | ||
<li class="category"><a href="#october">OCTOBER</a></li> | <li class="category"><a href="#october">OCTOBER</a></li> | ||
| - | |||
</ul> | </ul> | ||
<div class="bar"> | <div class="bar"> | ||
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<div id="january" class="container january"> | <div id="january" class="container january"> | ||
<h1 id="header1">January</h1> | <h1 id="header1">January</h1> | ||
| - | <p id="para"> | + | <p id="para">-Background research<br> |
</p> | </p> | ||
</div> | </div> | ||
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<div id="february" class="container february"> | <div id="february" class="container february"> | ||
<h1 id="header1">February</h1> | <h1 id="header1">February</h1> | ||
| - | <p id="para"> | + | <p id="para">-Background research<br> |
</p> | </p> | ||
</div> | </div> | ||
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<div id="march" class="container march"> | <div id="march" class="container march"> | ||
<h1 id="header1">March</h1> | <h1 id="header1">March</h1> | ||
| - | <p id="para"> | + | <p id="para"> |
| + | -Selection of topic<br> | ||
| + | -Further research<br> | ||
| + | -Selection of projec<br> | ||
</p> | </p> | ||
</div> | </div> | ||
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<div id="april" class="container april"> | <div id="april" class="container april"> | ||
<h1 id="header1">April</h1> | <h1 id="header1">April</h1> | ||
| - | <p id="para"> | + | <p id="para">-Further research <br> |
</p> | </p> | ||
</div> | </div> | ||
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<div id="may" class="container may"> | <div id="may" class="container may"> | ||
<h1 id="header1">May</h1> | <h1 id="header1">May</h1> | ||
| - | <p id="para"> | + | <p id="para"><b><u>General Activities</b></u><br> |
| + | Regular team meetings<br> | ||
| + | Looking for sponsors<br> | ||
| + | Month planning<br> | ||
</p> | </p> | ||
</div> | </div> | ||
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<div id="june" class="container june"> | <div id="june" class="container june"> | ||
<h1 id="header1">June</h1> | <h1 id="header1">June</h1> | ||
| - | <p id="para"> | + | <p id="para"><b><u>Wetlab</u></b><br> |
| + | - Arrival of the biobricks<br> | ||
| + | - The designs for the plasmids are approved and ordered Primers<br> | ||
| + | - Preparation of culture media, agar plates, antibiotics and glycerol<br> | ||
| + | - Amplification of PET-29a(+), pEVOL-pAzF, BBa_K811005(Penn) & CPX<br> | ||
| + | |||
</p> | </p> | ||
</div> | </div> | ||
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<div id="july" class="container july"> | <div id="july" class="container july"> | ||
<h1 id="header1">July</h1> | <h1 id="header1">July</h1> | ||
| - | <p id="para"> | + | <p id="para"><b><u>Wetlab</b></u><br> |
| + | <b>Week 27: 30 June – 6 July</b><br> | ||
| + | Plasmid amplification and culturing BBa_K811005(Penn) + CPX<br> | ||
| + | Vector and insert Digestions<br> | ||
| + | BBa_K811005(Penn) + CPX insert ligate pET29a <br> | ||
| + | Colony PCR <br> | ||
| + | <br> | ||
| + | <b>Week 28: 7 July – 13 July</b><br> | ||
| + | Colony PCR <br> | ||
| + | Site directed mutagenesis of CPX <br> | ||
| + | <br> | ||
| + | <b>Week 29: 14 July – 20 July</b><br> | ||
| + | Site directed mutagenesis of CPX <br> | ||
| + | Sequencing of CPX and BBa_K811005(Penn)<br> | ||
| + | <br> | ||
| + | <b>Week 30: 21 July – 27 July</b><br> | ||
| + | Sequencing results came in and were as expected<br> | ||
| + | Protein expression <br> | ||
| + | FACS – DBCO-PEG4-5/6-TAMRA <br> | ||
| + | <br> | ||
| + | <b>Week 31: 28 July – August 3</b><br> | ||
| + | Protein expression<br> | ||
| + | FACS – DBCO-PEG10kDa <br> | ||
| + | |||
</p> | </p> | ||
</div> | </div> | ||
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<div id="august" class="container august"> | <div id="august" class="container august"> | ||
<h1 id="header1">August</h1> | <h1 id="header1">August</h1> | ||
| - | <p id="para"> | + | <p id="para"><b><u>Wetlab</b></u><br> |
| + | <b>Week 32: 4 August – 10 August</b><br> | ||
| + | Protein expression <br> | ||
| + | FACS – Antibody titration <br> | ||
| + | Protein expression curve <br> | ||
| + | <br> | ||
| + | <b>Week 33: 11 August – 17 August</b><br> | ||
| + | FACS - DBCO PEG-5kDa fluorescent <br> | ||
| + | <br> | ||
| + | <b>Week 34: 18 August – 24 August</b><br> | ||
| + | Start Bio-bricking<br> | ||
| + | Start silencing mutagenesis <br> | ||
| + | <br> | ||
| + | <b>Week 35: 25 August – 31 August</b><br> | ||
</p> | </p> | ||
</div> | </div> | ||
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<div id="september" class="container september"> | <div id="september" class="container september"> | ||
<h1 id="header1">September</h1> | <h1 id="header1">September</h1> | ||
| - | <p id="para"> | + | <p id="para"><b><u>Wetlab</b></u><br> |
| + | <b>Week 36: 1 September – 7 September</b><br> | ||
| + | <br> | ||
| + | <b>Week 37: 8 September – 14 September</b><br> | ||
| + | <br> | ||
| + | <b>Week 38: 15 September – 21 September</b><br> | ||
| + | <br> | ||
| + | <b>Week 39: 22 September – 28 September</b><br> | ||
</p> | </p> | ||
</div> | </div> | ||
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<div id="october" class="container october"> | <div id="october" class="container october"> | ||
<h1 id="header1">October</h1> | <h1 id="header1">October</h1> | ||
| - | <p id="para"> | + | <p id="para"><b><u>Wetlab</b></u><br> |
| + | <b>Week 40: 29 September – 5 October</b><br> | ||
| + | <br> | ||
| + | <b>Week 41: 6 October – 12 October</b><br> | ||
| + | <br> | ||
| + | <b>Week 42: 13 October – 19 October</b><br> | ||
</p> | </p> | ||
</div> | </div> | ||
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<br><br> | <br><br> | ||
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Latest revision as of 12:58, 25 August 2014
Notebook
January
-Background research
February
-Background research
March
-Selection of topic
-Further research
-Selection of projec
April
-Further research
May
General Activities
Regular team meetings
Looking for sponsors
Month planning
June
Wetlab
- Arrival of the biobricks
- The designs for the plasmids are approved and ordered Primers
- Preparation of culture media, agar plates, antibiotics and glycerol
- Amplification of PET-29a(+), pEVOL-pAzF, BBa_K811005(Penn) & CPX
July
Wetlab
Week 27: 30 June – 6 July
Plasmid amplification and culturing BBa_K811005(Penn) + CPX
Vector and insert Digestions
BBa_K811005(Penn) + CPX insert ligate pET29a
Colony PCR
Week 28: 7 July – 13 July
Colony PCR
Site directed mutagenesis of CPX
Week 29: 14 July – 20 July
Site directed mutagenesis of CPX
Sequencing of CPX and BBa_K811005(Penn)
Week 30: 21 July – 27 July
Sequencing results came in and were as expected
Protein expression
FACS – DBCO-PEG4-5/6-TAMRA
Week 31: 28 July – August 3
Protein expression
FACS – DBCO-PEG10kDa
August
Wetlab
Week 32: 4 August – 10 August
Protein expression
FACS – Antibody titration
Protein expression curve
Week 33: 11 August – 17 August
FACS - DBCO PEG-5kDa fluorescent
Week 34: 18 August – 24 August
Start Bio-bricking
Start silencing mutagenesis
Week 35: 25 August – 31 August
September
Wetlab
Week 36: 1 September – 7 September
Week 37: 8 September – 14 September
Week 38: 15 September – 21 September
Week 39: 22 September – 28 September
October
Wetlab
Week 40: 29 September – 5 October
Week 41: 6 October – 12 October
Week 42: 13 October – 19 October
