Team:SF Bay Area DIYbio/Safety

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:SF_Bay_Area_DIYbio/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:SF_Bay_Area_DIYbio/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/Parts"style="color:#000000"> Parts</a></td>
<a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/Parts"style="color:#000000"> Parts</a></td>
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<a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/Modeling"style="color:#000000"> Modeling</a></td>
 
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<a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/Safety"style=" color:#000000"> Safety </a></td>
<a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/ELSEI"style=" color:#000000">Ethical, Legal, Societal, Environmental Issues</a></td>
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<a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/Attributions"style="color:#000000"> Attributions </a></td>
<a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/Attributions"style="color:#000000"> Attributions </a></td>
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<a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/Future_Plans"style="color:#000000"> Future Plans </a></td>
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<tr><td > <h3> Welcome! </h3></td>
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<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
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<h3> Edit this page!</h3>
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Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
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<li> <a href=" ">Learn about lab Safety for Today</a></li>
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<li> <a href="">Learn about Safety for the future of your project.</a></li>
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<h3> Your Lab </h3>
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<p> &nbsp; </p>
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<li> <b>Now :</b>  Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
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<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
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<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
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<li><b>June 9: </b>Submit the About Our Lab form.</li>
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<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
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<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
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<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
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<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
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<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
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<p><b><font size="5">SAFETY</font></b><br><br>
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<b>Lab safety considerations for this project</b><br><br>
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Team members have received safety training instruction relevant to specific experiments.  Both BioCurious and Counter Culture Labs have specific multi-person biosafety teams.  One of the team advisors, Patrik D’haeseleer, is on the safety team for both labs and has briefed both teams on the project. Both labs strictly meet the guidelines for BSL-1.
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The risks for members working on this project include the risk of RG1 lab strains.  There is no risk to the general public, since we are working with auxotrophic baker’s yeast carrying a plasmid expressing food proteins.
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To mitigate the risks to the environment from this project we are using yeast that can’t make its own uracil and must be grown on media containing supplement uracil.  This yeast strain is highly unlikely to be viable outside of specific lab conditions. Another safety precaution we have put in place to make sure our project stays contained is that only plasmids will be allowed to leave the lab and be transported from one location to the other – no live yeast or purified protein at this stage.
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As we work on the project we continue to address safety considerations and ensure that the experiments and work is done with best lab practices.
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Latest revision as of 00:07, 18 October 2014



Click here to edit this page!

 


SAFETY

Lab safety considerations for this project

Team members have received safety training instruction relevant to specific experiments. Both BioCurious and Counter Culture Labs have specific multi-person biosafety teams. One of the team advisors, Patrik D’haeseleer, is on the safety team for both labs and has briefed both teams on the project. Both labs strictly meet the guidelines for BSL-1.

The risks for members working on this project include the risk of RG1 lab strains. There is no risk to the general public, since we are working with auxotrophic baker’s yeast carrying a plasmid expressing food proteins. To mitigate the risks to the environment from this project we are using yeast that can’t make its own uracil and must be grown on media containing supplement uracil. This yeast strain is highly unlikely to be viable outside of specific lab conditions. Another safety precaution we have put in place to make sure our project stays contained is that only plasmids will be allowed to leave the lab and be transported from one location to the other – no live yeast or purified protein at this stage. As we work on the project we continue to address safety considerations and ensure that the experiments and work is done with best lab practices.


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