Latest revision as of 23:55, 17 October 2014

Dundee 2014

BioBricks

Just Another Brick in the Wall

Part:BBa_K1315001 (pqsA)

This is a promoter from Pseudomonas aeruginosa that is positively regulated when the transcription factor PqsR (BBa_K1157001) is activated by PQS (Pseudomonas Quinolone Signal).

Usage and Biology
The Pseudomonas quinolone signal (PQS) is imperative in modulating mechanisms that enable Pseudomonas aeruginosa to survive at times of stress. P. aeruginosa is the main pathogen responsible for severe deterioration in the Cystic Fibrosis lungs. The production of this signal relies on the pqsA operon. The transcriptional factor PqsR (BBa_K1315000) positively regulates this operon.

Recommended reading:
http://www.ncbi.nlm.nih.gov/pubmed/25225275
http://www.ncbi.nlm.nih.gov/pubmed/16735731
http://www.ncbi.nlm.nih.gov/pubmed/21539583
http://www.ncbi.nlm.nih.gov/pubmed/?term=pqsr-dependent+and+pqsr-independent+regulation+of+motility+and+biofilm+formation+by+pqs
http://www.ncbi.nlm.nih.gov/pubmed/12393198

Part:BBa_K1315002 (rpfC)

This is the coding sequence for the RpfC (regulation of pathogenicity factor C) protein. It is the sensor in the two-component signal transduction system of Xanthomonas campestris pv. campestris that activates the regulator RpfG (BBa_K1315003) in the presence of DSF (diffusible signal factor). RpfC eventually leads to the activation of the manA promoter (BBa_K1315006).

Usage and Biology
Quorum sensing and bacterial cell signalling are mechanisms known to be utilised by bacteria to regulate their virulence. The RpfC-RpfG sensory system has been found to activate the production of virulence factors like extracellular enzymes and polysaccharides in Xanthomonas campestris. It has been also suggested that this system is involved in linking environmental stimuli, including DSF, with pathogenicity in Xanthomonas campestris. DSF is a cis-unsaturated fatty acid from the DSF family. The RpfC-RpfG two-component system is the most well understood one and is present in all Xanthomonads.

Recommended reading:
http://www.ncbi.nlm.nih.gov/pubmed/?term=r+ryan+dsf+family
http://www.ncbi.nlm.nih.gov/pubmed/?term=A+two-component+system+involving+an+HD-GYP+domain+protein+links+cell%E2%80%93cell+signalling+to+pathogenicity+gene+expression+in+Xanthomonas+campestris

Part:BBa_K1315003 (rpfG)

This is the coding sequence for the RpfG (regulation of pathogenicity factor G) protein. It is the regulator in the two-component signal transduction system of Xanthomonas campestris pv. campestris that is activated by the sensor kinase RpfC (BBa_K1315002) in the presence of DSF (diffusible signal factor). RpfG eventually leads to the activation of the manA promoter (BBa_K1315006).

Usage and Biology
Quorum sensing and bacterial cell signalling are mechanisms known to be utilised by bacteria to regulate their virulence. The RpfC-RpfG sensory system has been found to activate the production of virulence factors like extracellular enzymes and polysaccharides in Xanthomonas campestris. It has been also suggested that this system is involved in linking environmental stimuli, including DSF, with pathogenicity in Xanthomonas campestris. DSF is a cis-unsaturated fatty acid from the DSF family. The RpfC-RpfG two-component system is the most well understood one and is present in all Xanthomonads.

Recommended reading:
http://www.ncbi.nlm.nih.gov/pubmed/?term=r+ryan+dsf+family
http://www.ncbi.nlm.nih.gov/pubmed/?term=A+two-component+system+involving+an+HD-GYP+domain+protein+links+cell%E2%80%93cell+signalling+to+pathogenicity+gene+expression+in+Xanthomonas+campestris

Part:BBa_K1315005 (clp)

This is the coding sequence for the cAMP receptor-like protein (Clp). This is a transcriptional activator that is normally inhibited by cyclic di-GMP. Inhibition is alleviated by the RpfG regulator (BBa_K1315003) of the RpfC-RpfG two-component system.

Usage and Biology
Quorum sensing and bacterial cell signalling are mechanisms known to be utilised by bacteria to regulate their virulence. The RpfC-RpfG sensory system has been found to activate the production of virulence factors like extracellular enzymes and polysaccharides in Xanthomonas campestris. It has been also suggested that this system is involved in linking environmental stimuli, including DSF, with pathogenicity in Xanthomonas campestris. DSF is a cis-unsaturated fatty acid from the DSF family. The RpfC-RpfG two-component system is the most well understood one and is present in all Xanthomonads. When RpfC (BBa_K1315003) picks up DSF, it eventually signals RpfG (BBa_K1315003) to degrade cyclic di-GMP. This stops the inhibition of Clp and the transcriptional activator can activate genes.

Recommended reading:
http://www.ncbi.nlm.nih.gov/pubmed/?term=r+ryan+dsf+family
http://www.ncbi.nlm.nih.gov/pubmed/?term=A+two-component+system+involving+an+HD-GYP+domain+protein+links+cell%E2%80%93cell+signalling+to+pathogenicity+gene+expression+in+Xanthomonas+campestris

Part:BBa_K1315006 (manA)

This is the manA gene promoter taken from Xanthomonas campestris pv. campestris. It is activated by the transcriptional activator Clp (BBa_K1315005).

Usage and Biology
The DSF-RpfC (BBa_K1315002)-RpfG (BBa_K1315003) sensory system, in all Xanthomonads, activates the production of the manA gene, which codes for endo-mannanase. It has been shown that the transcriptional regulator Clp (BBa_K1315005), when activated binds to the promoter of manA and turns on its synthesis. Clp is usually inactive by cyclic di-GMP molecules that hinder the ability of the transcriptional factor to bind to DNA.

Recommended reading:
http://www.ncbi.nlm.nih.gov/pubmed/23285129
http://www.ncbi.nlm.nih.gov/pubmed/20073482

Part:BBa_K1315007 (BCAM0228)

This is the coding sequence of the BCAM0228 two-component regulator of the BDSF sensory BCAM0227-BCAM0228 system. This system is found in Burkholderia cenocepacia. It is suggested that it activates the cblD promoter (BBa_K1315008).

Usage and Biology
The Burkholderia diffusible signal factor (BDSF/cis-2-dodecenoic acid) is a signalling molecule that regulates virulence in Burkholderia cenocepacia. BDSF belongs to the DSF family which was first described in Xanthomonas campestris. BCAM0227 has been identified as a sensor kinase of the BCAM0227-BCAM0228 system that picks up BDSF. However, the confirmed BDSF signalling pathway only goes as far as BCAM0227. It has been proposed that the next steps involve the BCAM0228 regulator protein. The BCAM0228 regulator though is more similar to the OmpR two-component response regulator rather the RpfG (BBa_K1315003) one. Thus, BCAM0228 is the hypothetical regulator of the BCAM0227-BCAM0228 system.

Recommended reading:
http://www.ncbi.nlm.nih.gov/pubmed/21227698
http://www.ncbi.nlm.nih.gov/pubmed/?term=A+sensor+kinase+recognizing+the+cell-cell+signal+BDSF+(cis-2-dodecenoic+acid)+regulates+virulence+in+Burkholderia+cenocepacia.

Part:BBa_K1315008 (cblD)

This is a promoter that is activated by the OmpR-like two-component response regulator BCAM0228 (BBa_K1315007) in the BCAM0227-BCAM0228 sensor system. The Burkholderia diffusible signal (BDSF) is picked up by the BCAM0227 sensor kinase that is hypothesised to eventually lead in involvement to the phosphorylation of BCAM0228. BCAM0228 then activates transcription from the cblD promoter.

Part:BBa_K1315009

PAI-1 Biosensor 1 (GFP readout)
This is a composite part that consists of 4 biobricks. In the front it has P_tet (BBa_R0040) which is constitutively on. This produces LasR all the time with lasR (BBa_C0179) next in the sequence. Only in the presence of the Pseudomonas Autoinducer 1 (PAI-1), LasR forms an complex with PAI-1 and it can then activate the P_lasB (BBa_R0079) promoter that leads to the production of GFP (BBa_E0040).

Part:BBa_K1315010

PAI-1 Biosensor 2 (GFP readout)
This is a composite part that consists of 4 biobricks. This is an alternative PAI-1 Activated System from BBa_K1315009 that instead uses P_luxR (BBa_R0062). In the front it has P_tet (BBa_R0040) which is constitutively on. This produces LasR all the time with lasR (BBa_C0179) next in the sequence. Only in the presence of the Pseudomonas Autoinducer 1 (PAI-1), LasR forms an complex with PAI-1 and it can then activate the P_luxR (BBa_R0062) promoter that leads to the production of GFP (BBa_E0040).

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+<div class="container">
+    <div class="jumbotron">
+              <h1>BioBricks</h1>
+<p class="lead">Just Another Brick in the Wall</p>
+    </div>
-<!--welcome box -->
-<tr>
-<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
-<h1 >WELCOME TO iGEM 2014! </h1>
+<div class="row">
-<p>Your team has been approved and you are ready to start the iGEM season!
+<div class="col-12">
-<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
+<hr>
+<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1315001"target="_blank">Part:BBa_K1315001</a> (<i>pqsA</i>)</h2>
+<p>
+This is a promoter from <i>Pseudomonas aeruginosa</i> that is positively regulated when the transcription factor PqsR (BBa_K1157001) is activated by PQS (<i>Pseudomonas</i> Quinolone Signal).
+<br/>
+<br/>
+<b>Usage and Biology</b>
 <br>
-<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Dundee/Parts&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
+The <i>Pseudomonas</i> quinolone signal (PQS) is imperative in modulating mechanisms that enable <i>Pseudomonas aeruginosa</i> to survive at times of stress. <i>P. aeruginosa</i> is the main pathogen responsible for severe deterioration in the Cystic Fibrosis lungs. The production of this signal relies on the <i>pqsA</i> operon. The transcriptional factor PqsR (BBa_K1315000) positively regulates this operon.
-</td>
+<br/>
-</tr>
+<br/>
-<tr> <td colspan="3"  height="5px"> </td></tr>
+Recommended reading:
-<!-- end welcome box -->
+<br/>
-<tr>
-<!--navigation menu -->
+<a href="http://www.ncbi.nlm.nih.gov/pubmed/25225275">http://www.ncbi.nlm.nih.gov/pubmed/25225275</a><br/>
-<td align="center" colspan="3">
+<a href="http://www.ncbi.nlm.nih.gov/pubmed/16735731">http://www.ncbi.nlm.nih.gov/pubmed/16735731</a><br/>
+<a href="http://www.ncbi.nlm.nih.gov/pubmed/21539583">http://www.ncbi.nlm.nih.gov/pubmed/21539583</a><br/>
+<a href="http://www.ncbi.nlm.nih.gov/pubmed/?term=pqsr-dependent+and+pqsr-independent+regulation+of+motility+and+biofilm+formation+by+pqs">http://www.ncbi.nlm.nih.gov/pubmed/?term=pqsr-dependent+and+pqsr-independent+regulation+of+motility+and+biofilm+formation+by+pqs</a><br/>
+<a href="http://www.ncbi.nlm.nih.gov/pubmed/12393198">http://www.ncbi.nlm.nih.gov/pubmed/12393198</a><br/>
-<table  width="100%">
+</p>
-<tr heigth="15px"></tr>
+</div>
-<tr heigth="75px">
+</div>
-<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+<div class="row">
-<a href="https://2014.igem.org/Team:Dundee"style="color:#000000">Home </a> </td>
+<div class="col-12">
+<hr>
+<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1315002"target="_blank">Part:BBa_K1315002</a> (<i>rpfC</i>)</h2>
+<p>
-<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+This is the coding sequence for the RpfC (regulation of pathogenicity factor C) protein. It is the sensor in the two-component signal transduction system of <i>Xanthomonas campestris</i> pv. <i>campestris</i> that activates the regulator RpfG (BBa_K1315003) in the presence of DSF (diffusible signal factor). RpfC eventually leads to the activation of the <i>manA</i> promoter (BBa_K1315006).
-<a href="https://2014.igem.org/Team:Dundee/Team"style="color:#000000"> Team </a> </td>
+<br/>
+<br/>
+<b>Usage and Biology</b>
+<br/>
+Quorum sensing and bacterial cell signalling are mechanisms known to be utilised by bacteria to regulate their virulence. The RpfC-RpfG sensory system has been found to activate the production of virulence factors like extracellular enzymes and polysaccharides in <i>Xanthomonas campestris</i>. It has been also suggested that this system is involved in linking environmental stimuli, including DSF, with pathogenicity in <i>Xanthomonas campestris</i>. DSF is a cis-unsaturated fatty acid from the DSF family. The RpfC-RpfG two-component system is the most well understood one and is present in all Xanthomonads.
+<br/>
+<br/>
+Recommended reading:
+<br/>
+<a href="http://www.ncbi.nlm.nih.gov/pubmed/?term=r+ryan+dsf+family">http://www.ncbi.nlm.nih.gov/pubmed/?term=r+ryan+dsf+family</a><br/>
+<a href=" http://www.ncbi.nlm.nih.gov/pubmed/?term=A+two-component+system+involving+an+HD-GYP+domain+protein+links+cell%E2%80%93cell+signalling+to+pathogenicity+gene+expression+in+Xanthomonas+campestris"> http://www.ncbi.nlm.nih.gov/pubmed/?term=A+two-component+system+involving+an+HD-GYP+domain+protein+links+cell%E2%80%93cell+signalling+to+pathogenicity+gene+expression+in+Xanthomonas+campestris</a><br/><a
-<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
-<a href="https://igem.org/Team.cgi?year=2014&team_name=Dundee"style="color:#000000"> Official Team Profile </a></td>
-<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+</p>
-<a href="https://2014.igem.org/Team:Dundee/Project"style="color:#000000"> Project</a></td>
+</div>
+</div>
-<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+<div class="row">
-<a href="https://2014.igem.org/Team:Dundee/Parts"style="color:#000000"> Parts</a></td>
+<div class="col-12">
+<hr>
+<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1315003"target="_blank">Part:BBa_K1315003</a> (<i>rpfG</i>)</h2>
+<p>
+This is the coding sequence for the RpfG (regulation of pathogenicity factor G) protein. It is the regulator in the two-component signal transduction system of <i>Xanthomonas campestris</i> pv. <i>campestris</i> that is activated by the sensor kinase RpfC (BBa_K1315002) in the presence of DSF (diffusible signal factor). RpfG eventually leads to the activation of the <i>manA</i> promoter (BBa_K1315006).
+<br/>
+<br/>
+<b>Usage and Biology</b>
+<br/>
+Quorum sensing and bacterial cell signalling are mechanisms known to be utilised by bacteria to regulate their virulence. The RpfC-RpfG sensory system has been found to activate the production of virulence factors like extracellular enzymes and polysaccharides in <i>Xanthomonas campestris</i>. It has been also suggested that this system is involved in linking environmental stimuli, including DSF, with pathogenicity in <i>Xanthomonas campestris</i>. DSF is a <i>cis</i>-unsaturated fatty acid from the DSF family. The RpfC-RpfG two-component system is the most well understood one and is present in all Xanthomonads.
+<br/>
+<br/>
+Recommended reading:
+<br/>
-<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+<a href="http://www.ncbi.nlm.nih.gov/pubmed/?term=r+ryan+dsf+family">http://www.ncbi.nlm.nih.gov/pubmed/?term=r+ryan+dsf+family</a><br/>
-<a href="https://2014.igem.org/Team:Dundee/Modeling"style="color:#000000"> Modeling</a></td>
+<a href="http://www.ncbi.nlm.nih.gov/pubmed/?term=A+two-component+system+involving+an+HD-GYP+domain+protein+links+cell%E2%80%93cell+signalling+to+pathogenicity+gene+expression+in+Xanthomonas+campestris">http://www.ncbi.nlm.nih.gov/pubmed/?term=A+two-component+system+involving+an+HD-GYP+domain+protein+links+cell%E2%80%93cell+signalling+to+pathogenicity+gene+expression+in+Xanthomonas+campestris</a><br/>
-<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
-<a href="https://2014.igem.org/Team:Dundee/Notebook"style="color:#000000"> Notebook</a></td>
+</p>
+</div>
+</div>
-<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
-<a href="https://2014.igem.org/Team:Dundee/Safety"style=" color:#000000"> Safety </a></td>
-<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
-<a href="https://2014.igem.org/Team:Dundee/Attributions"style="color:#000000"> Attributions </a></td>
+<div class="row">
+<div class = "col-12">
+<hr>
+<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1315005"target="_blank">Part:BBa_K1315005</a> (<i>clp</i>)</h2>
+<p>
-<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
+This is the coding sequence for the cAMP receptor-like protein (Clp). This is a transcriptional activator that is normally inhibited by cyclic di-GMP. Inhibition is alleviated by the RpfG regulator (BBa_K1315003) of the RpfC-RpfG two-component system.
-</tr>
+<br/>
-</table>
+<br/>
+<b>Usage and Biology</b>
+<br/>
+Quorum sensing and bacterial cell signalling are mechanisms known to be utilised by bacteria to regulate their virulence. The RpfC-RpfG sensory system has been found to activate the production of virulence factors like extracellular enzymes and polysaccharides in <i>Xanthomonas campestris</i>. It has been also suggested that this system is involved in linking environmental stimuli, including DSF, with pathogenicity in <i>Xanthomonas campestris</i>. DSF is a cis-unsaturated fatty acid from the DSF family. The RpfC-RpfG two-component system is the most well understood one and is present in all Xanthomonads. When RpfC (BBa_K1315003) picks up DSF, it eventually signals RpfG (BBa_K1315003) to degrade cyclic di-GMP. This stops the inhibition of Clp and the transcriptional activator can activate genes.
+<br/>
+<br/>
+Recommended reading:
+<br/>
-<!--end navigation menu -->
+<a href="http://www.ncbi.nlm.nih.gov/pubmed/?term=r+ryan+dsf+family">http://www.ncbi.nlm.nih.gov/pubmed/?term=r+ryan+dsf+family</a><br/><a href="http://www.ncbi.nlm.nih.gov/pubmed/?term=A+two-component+system+involving+an+HD-GYP+domain+protein+links+cell%E2%80%93cell+signalling+to+pathogenicity+gene+expression+in+Xanthomonas+campestris">http://www.ncbi.nlm.nih.gov/pubmed/?term=A+two-component+system+involving+an+HD-GYP+domain+protein+links+cell%E2%80%93cell+signalling+to+pathogenicity+gene+expression+in+Xanthomonas+campestris</a><br/>
-</tr>
+ <br/>
-</tr>
+</p>
+</div>
+</div>
+<div class="row">
+<div class = "col-12">
+<hr>
+<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1315006"target="_blank">Part:BBa_K1315006</a> (<i>manA</i>)</h2>
+This is the <i>manA</i> gene promoter taken from <i>Xanthomonas campestris</i> pv. <i>campestris</i>. It is activated by the transcriptional activator Clp (BBa_K1315005).
+<br/>
+<br/>
+<b>Usage and Biology</b>
+<br>
+The DSF-RpfC (BBa_K1315002)-RpfG (BBa_K1315003) sensory system, in all Xanthomonads, activates the production of the <i>manA</i> gene, which codes for endo-mannanase. It has been shown that the transcriptional regulator Clp (BBa_K1315005), when activated binds to the promoter of <i>manA</i> and turns on its synthesis. Clp is usually inactive by cyclic di-GMP molecules that hinder the ability of the transcriptional factor to bind to DNA.
+<br/>
+<br/>
-</td>
+Recommended reading:
+<br/>
-<tr> <td colspan="3"  height="15px"> </td></tr>
-<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
-<tr> <td colspan="3"  height="5px"> </td></tr>
+<a href="
+http://www.ncbi.nlm.nih.gov/pubmed/23285129">http://www.ncbi.nlm.nih.gov/pubmed/23285129</a><br/><a
+<a href="
+ http://www.ncbi.nlm.nih.gov/pubmed/20073482"> http://www.ncbi.nlm.nih.gov/pubmed/20073482</a><br/><a
-<!--Parts Submitted to the Registry  -->
-<tr><td > <h3> Parts Submitted to the Registry </h3></td>
-<td ></td >
-<td > <h3>What information do I need to start putting my parts on the Registry? </h3></td>
-</tr>
-<tr>
-<td width="45%"  valign="top">
-<p>
-An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will submit them to the <a href="http://partsregistry.org"> Registry of Standard Biological Parts</a>. The iGEM software provides an easy way to present the parts your team has created. The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.
-<p>
-<strong>Note that if you want to document a part you need to document it on the <a href="http://partsregistry.org Registry"> Registry</a>, not on your team wiki.</strong> Future teams and other users and are much more likely to find parts on the Registry than on your team wiki.
 </p>
+</div>
+</div>
+<div class="row">
+<div class="col-12">
+<hr>
+<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1315007"target="_blank">Part:BBa_K1315007</a> (<i>BCAM0228</i>) </h2>
 <p>
-Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without a need to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.
-</p>
+This is the coding sequence of the BCAM0228 two-component regulator of the BDSF sensory BCAM0227-BCAM0228 system. This system is found in <i>Burkholderia cenocepacia</i>. It is suggested that it activates the <i>cblD</i> promoter (BBa_K1315008).
+<br/>
+<br/>
+<b>Usage and Biology</b>
+<br/>
+The <i>Burkholderia</i> diffusible signal factor (BDSF/cis-2-dodecenoic acid) is a signalling molecule that regulates virulence in <i>Burkholderia cenocepacia</i>. BDSF belongs to the DSF family which was first described in <i>Xanthomonas campestris</i>. BCAM0227 has been identified as a sensor kinase of the BCAM0227-BCAM0228 system that picks up BDSF.
+However, the confirmed BDSF signalling pathway only goes as far as BCAM0227. It has been proposed that the next steps involve the BCAM0228 regulator protein. The BCAM0228 regulator though is more similar to the OmpR two-component response regulator rather the RpfG (BBa_K1315003) one. Thus, BCAM0228 is the hypothetical regulator of the BCAM0227-BCAM0228 system.
+<br/>
+<br/>
+Recommended reading:
+<br/>
+<a href="http://www.ncbi.nlm.nih.gov/pubmed/21227698">http://www.ncbi.nlm.nih.gov/pubmed/21227698</a><br/><a
-<h3>When should you put parts into the Registry?</h3>
+<a href=" http://www.ncbi.nlm.nih.gov/pubmed/?term=A+sensor+kinase+recognizing+the+cell-cell+signal+BDSF+(cis-2-dodecenoic+acid)+regulates+virulence+in+Burkholderia+cenocepacia."> http://www.ncbi.nlm.nih.gov/pubmed/?term=A+sensor+kinase+recognizing+the+cell-cell+signal+BDSF+(cis-2-dodecenoic+acid)+regulates+virulence+in+Burkholderia+cenocepacia.</a><br/><a
-<p>
-As soon as possible! We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better recall you will have of all details surrounding your parts. Remember you don't need to send us the DNA to create an entry for a part on the Registry. However, you must send us the sample/DNA before the Jamboree. Only parts for which you have sent us samples/DNA are eligible for awards and medal requirements.
-</p>
-</td>
-<td > </td>
-<td width="45%" valign="top">
-<p>
+<br/>
-The information needed to initially create a part on the Registry is:
 </p>
-<ol>
+</div>
+</div>
-<li>Part Name</li>
-<li>Part type</li>
-<li>Creator</li>
-<li>Sequence</li>
-<li>Short Description (60 characters on what the DNA does)</li>
-<li>Long Description (Longer description of what the DNA does)</li>
-<li>Design considerations</li>
-</ol>
+<div class = "row">
+<div class = "col-12">
+<hr>
+<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1315008"target="_blank">Part:BBa_K1315008</a> (<i>cblD</i>)</h2>
 <p>
-We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. Check out part <a href="http://parts.igem.org/Part:BBa_K404003">BBa_K404003</a> for an excellent example of a highly characterized part.
+This is a promoter that is activated by the OmpR-like two-component response regulator BCAM0228 (BBa_K1315007) in the BCAM0227-BCAM0228 sensor system. The <i>Burkholderia</i> diffusible signal (BDSF) is picked up by the BCAM0227 sensor kinase that is hypothesised to eventually lead in involvement to the phosphorylation of BCAM0228. BCAM0228 then activates transcription from the <i>cblD</i> promoter.
 </p>
+</div>
+</div>
+<div class = "row">
+<div class = "col-12">
+<hr>
+<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1315009"target="_blank">Part:BBa_K1315009</a></h2>
 <p>
-You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry"> Add a Part to the Registry</a> link.
+<b>PAI-1 Biosensor 1 (GFP readout)</b>
+<br/>
+This is a composite part that consists of 4 biobricks. In the front it has P<i><sub>tet</sub></i> (BBa_R0040) which is constitutively on. This produces LasR all the time with <i>lasR</i> (BBa_C0179) next in the sequence. Only in the presence of the <i>Pseudomonas</i> Autoinducer 1 (PAI-1), LasR forms an complex with PAI-1 and it can then activate the P<i><sub>lasB</sub></i> (BBa_R0079) promoter that leads to the production of GFP (BBa_E0040).
 </p>
-</td>
+</div>
-</tr>
+</div>
-<tr> <td colspan="3"  height="15px"> </td></tr>
-<tr><td colspan="3" > <h3> Parts Table</h3></td></tr>
+<div class = "row">
+<div class = "col-12">
+<hr>
+<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1315010"target="_blank">Part:BBa_K1315010</a></h2>
+<p>
+<b>PAI-1 Biosensor 2 (GFP readout)</b>
+<br/>
+This is a composite part that consists of 4 biobricks. This is an alternative PAI-1 Activated System from BBa_K1315009 that instead uses P<i><sub>luxR</sub></i> (BBa_R0062). In the front it has P<i><sub>tet</sub></i> (BBa_R0040) which is constitutively on. This produces LasR all the time with <i>lasR</i> (BBa_C0179) next in the sequence. Only in the presence of the <i>Pseudomonas</i> Autoinducer 1 (PAI-1), LasR forms an complex with PAI-1 and it can then activate the P<i><sub>luxR</sub></i> (BBa_R0062) promoter that leads to the production of GFP (BBa_E0040).
+</p>
+</div>
+</div>
-<tr><td width="45%" colspan="3"  valign="top">
+<script>
-Any parts your team has created will appear in this table below:</td></tr>
+        $( "#info" ).click( function() {
+        $('#welcomeNote').appendTo("body");
+  $("#welcomeNote").modal();
+});
+</script>
-</table>
+</body>
 </html>
-<groupparts>iGEM013 Dundee</groupparts>
+{{:Team:Dundee/Template/welcomeNoteBricks}}
+<groupparts>iGEM014 Dundee</groupparts>