Team:Warwick/Attributions

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            <li> <a href = "/Team:Warwick"> HOME </a> </li>
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            <li> <a href = "/Team:Warwick/Project"> PROJECT </a> </li>
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            <li> <a href = "/Team:Warwick/Team"> TEAM </a> </li>
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            <li> <a href = "/Team:Warwick/Parts"> PARTS </a> </li>
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            <li> <a href = "/Team:Warwick/Modelling"> MODELLING </a> </li>
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            <li> <a href = "/Team:Warwick/Notebook"> NOTEBOOK </a> </li>
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            <!--<li> <a href = "/Team:Warwick/Measurements"> MEASUREMENTS </a> </li>-->
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            <li> <a href = "/Team:Warwick/Interlab"> INTERLAB </a> </li>
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            <li> <a href = "/Team:Warwick/Attributions"> <span> ATTRIBUTIONS </span> </a> </li>
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<li> <a href = "/Team:Warwick/Attributions"> <span>SPONSORS</span> </a> </li>
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<li> <a href = "/Team:Warwick/Attributions/Collaboration"> COLLABORATION </a> </li>
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<td align ="right"><a href="http://www.erasynbio.eu/"><img src="https://static.igem.org/mediawiki/2014/3/39/ESB_Final.png" width="410px"></a> </td>
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Our modelling in this project has several aims :
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<li>To find the amount of DPP-IV reduction reached when the system reaches equilibrium</li>
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<li>To find a way to control the level of DPP-IV reduction</li>
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<li>To find the minimum number of RdRps, replicons, etc to be initially transfected into the cell, which are required to achieve a steady state for the system</li>
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<li>To find out how long does it take for the system to reach equilibrium</li>
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<li>To find out the level of reduction we need to treat diabetes</li>
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<li>To find out how stable the system is (i.e. will the system only work in very specific situations, or in lots of different systems?)
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We are currently using Simbiology in Matlab and Copasi to model the system. We are currently adapting several different models, which come from research into HCV replicons, to our system. If our models can be made to fit our experiments well, we may extend our project to try and find a way to control the level of DPP-IV which is reduced. In addition modelling the system will allow it to be better optimised in the future, and optimum values for constants such as the strength of the ribosome binding sites, and the number of siRNAs produced by each degradation, so that the effect of our biobrick can be optimised.<p><p>
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Initially we determined that our system should reach some equilibrium after a certain amount of time. This is because firstly, HCV is a successful virus, so the replicons should not completely degrade away as time goes to infinity.  Secondly, since there are only a finite amount of resources within the cell, the number of replicons in the system cannot keep increasing forever. This means either the number of replicons must tend towards a certain constant (constant with respect to time), or the number of replicons should tend towards oscillations. <p><p>
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<p><b><ins><font size="4">Sponsorship Brochure</b></font></ins> <br>To help in securing sponsorship this year, the Warwick iGEM team created a brochure to send to companies and organisations. We upload it <a href = "https://static.igem.org/mediawiki/2014/b/bb/SponsorshipBrochure_%281%29.pdf"> here </a> in the hope that it helps inspire teams to create a similar document so that they can secure much needed funding and equipment as we did in the future. </p><br><br>
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<p><b><ins><font size="4">Further Attributions</b></font></ins><br>
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Thank you to the laboratory of Alfonso Jaramillo for allowing us to use the TECAN machine, lab space and helping us make RdRp biobrick compatible, as well as donating the design for our mutant RdRp to us. Also thanks to the laboratory of David Evans, who kindly donated HuH7 cells and HeLa cells to us so that we could perform many of our experiments. Thanks to Sian Davies for teaching us the protocols we needed, and for the shedloads of support throughout the project, as well as William Rostain and Satya Prakash for help with initial results analysis and general help throughout the project. Finally thanks to Alfonso Jaramillo for bringing the team together in the first place, and making this all possible.
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Latest revision as of 03:30, 18 October 2014

Sponsors




Sponsorship Brochure
To help in securing sponsorship this year, the Warwick iGEM team created a brochure to send to companies and organisations. We upload it here in the hope that it helps inspire teams to create a similar document so that they can secure much needed funding and equipment as we did in the future.



Further Attributions
Thank you to the laboratory of Alfonso Jaramillo for allowing us to use the TECAN machine, lab space and helping us make RdRp biobrick compatible, as well as donating the design for our mutant RdRp to us. Also thanks to the laboratory of David Evans, who kindly donated HuH7 cells and HeLa cells to us so that we could perform many of our experiments. Thanks to Sian Davies for teaching us the protocols we needed, and for the shedloads of support throughout the project, as well as William Rostain and Satya Prakash for help with initial results analysis and general help throughout the project. Finally thanks to Alfonso Jaramillo for bringing the team together in the first place, and making this all possible.

Retrieved from "http://2014.igem.org/Team:Warwick/Attributions"