Team:UiOslo Norway/Safety

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<h1 style="font-family:Syncopate; color: blue;">Safety</h1>
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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:UiOslo_Norway/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:UiOslo_Norway"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:UiOslo_Norway/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=UiOslo_Norway"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:UiOslo_Norway/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:UiOslo_Norway/Attributions"style="color:#000000"> Attributions </a></td>
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<tr><td > <h3> Welcome! </h3></td>
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<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
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<h3> Edit this page!</h3>
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Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
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<li> <a href=" ">Learn about lab Safety for Today</a></li>
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<li> <a href="">Learn about Safety for the future of your project.</a></li>
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<h3> Your Lab </h3>
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<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>
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Image:Example2_Lab_3.png|Team Member 3 doing an experiment
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Image:Example2_Lab_4.png|Working in biosafety cabinets
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<li> <b>Now :</b>  Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
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<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
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<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
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<li><b>June 9: </b>Submit the About Our Lab form.</li>
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<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
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<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
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<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
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<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
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<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
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<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
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<p>When working with bacteria and DNA safety is very important. Therefore we have taken necessary safety precautions when working in the lab. This includes:</p>
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<li><strong>Gloves</strong> and <strong>lab coats</strong> for our own protection.</li>
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<li><strong>Bacterial waste</strong> cans with plastic bags that are destroyed.</li>
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<li><strong>Bacterial liquid waste containers</strong> that are mixed with chlorine bleach before disposal in the lab sink.</li>
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<li><strong>Fume hoods</strong> when using strong chemicals.</li>
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<li><strong>Regular cleaning</strong> and <strong>disinfection</strong> of work space.</li>
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<p>In addition to obligatory safety courses for every master student working in labs (including fire safety, first aid and lab safety), the team got a brief course in lab safety by our supervisor before we started our lab work. This included safe deposition of waste, how to avoid spillage and when to use gloves.</p>
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<p>All the bacteria we have been working with are lab strains of safety level 1. We have been working in a lab with safety level 2. No GMO’s were allowed to leave the laboratorium.</p>
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<p>We should keep in mind that this project attempts to change the surfaces of bacteria, enabling the bacteria to stick to “complementary” surfaces. If pathogenic bacteria acquire such a capability by taking up our plasmids, they in will in principle broaden their ability of surface binding. This probability of this having any real effects is deemed very low, however.</p>
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Latest revision as of 14:21, 17 October 2014

UiOslo IGEM 2014

Safety

When working with bacteria and DNA safety is very important. Therefore we have taken necessary safety precautions when working in the lab. This includes:

  • Gloves and lab coats for our own protection.
  • Bacterial waste cans with plastic bags that are destroyed.
  • Bacterial liquid waste containers that are mixed with chlorine bleach before disposal in the lab sink.
  • Fume hoods when using strong chemicals.
  • Regular cleaning and disinfection of work space.

In addition to obligatory safety courses for every master student working in labs (including fire safety, first aid and lab safety), the team got a brief course in lab safety by our supervisor before we started our lab work. This included safe deposition of waste, how to avoid spillage and when to use gloves.

All the bacteria we have been working with are lab strains of safety level 1. We have been working in a lab with safety level 2. No GMO’s were allowed to leave the laboratorium.

We should keep in mind that this project attempts to change the surfaces of bacteria, enabling the bacteria to stick to “complementary” surfaces. If pathogenic bacteria acquire such a capability by taking up our plasmids, they in will in principle broaden their ability of surface binding. This probability of this having any real effects is deemed very low, however.