Team:LA Biohackers/Safety

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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:LA_Biohackers/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:LA_Biohackers/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<tr><td > <h3> Welcome! </h3></td>
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<tr><td > <h3>Safety isn't expensive. It's priceless! (author unknown)</h3></td>
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<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
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<p> Ours is an open community lab, and as such care was taken to choose a project that involved little risk. E. coli, S. thermophilus, and B. subtillus are all bacteria in the lowest risk group; Risk Group 1.  Wild type strains of these microorganism are naturally found in humans (E. coli) yogurt (S. thermophilus) and the soil (B. subtillis). 
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Even though these microbes have benign reputations, standard laboratory precautions were followed. The lab has plenty of gloves, safety glasses and lab coats, and access to running water in a shared bathroom. Media and glassware are sterilized with a pressure cooker (eBay).  
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Standard microbiological practices regarding eating, hand washing, and disinfecting surfaces were introduced at a lab meeting in July.  CDC guidelines were covered at the same meeting, and a self-test was administered after the presentation.
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The lab scrounged a Biological Safety Cabinent via Craig’s List and spent a considerable amount of time scheming ways to elevate it’s 550lbs from the floor to its stand. Solution: material lift from Home Depot.
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<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>
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<li> <b>Now :</b>  Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
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<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
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<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
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<li><b>June 9: </b>Submit the About Our Lab form.</li>
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<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
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<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
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<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
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<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
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<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
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<p> The WHO Biosafety Manual was assigned to be read by all team members. We completed the CDC Interactive Training Recognizing Biosafety  Levels training and quiz as a group. Our team presented real world case studies of the 2008 UCLA Lab fire death and subsequent felony OSHA prosecution, as well as the 2012 case of a VA lab worker in San Francisco who died after he was exposed to meningitis in the VA lab. Training covered PPE, instruction on our Biosafety cabinet and safe lab procedures with regard to carbon monoxide, explosive gases, fires, liquid nitrogen, and lasers. We discussed how to recognize and minimize risk by experimental design by using a real world example of how to determine the LEL of a explosive gas and minimize its danger by constructing a safe bioreactor. We have a fire extinguisher. The lab is on the 6th floor, and can be exited via elevator, stairs, and a fire escape.</p>
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Latest revision as of 03:51, 18 October 2014


Click here to edit this page!

Home Team Official Team Profile Project Parts Safety Practice and Policy

Safety isn't expensive. It's priceless! (author unknown)

Ours is an open community lab, and as such care was taken to choose a project that involved little risk. E. coli, S. thermophilus, and B. subtillus are all bacteria in the lowest risk group; Risk Group 1. Wild type strains of these microorganism are naturally found in humans (E. coli) yogurt (S. thermophilus) and the soil (B. subtillis).

Even though these microbes have benign reputations, standard laboratory precautions were followed. The lab has plenty of gloves, safety glasses and lab coats, and access to running water in a shared bathroom. Media and glassware are sterilized with a pressure cooker (eBay).

Standard microbiological practices regarding eating, hand washing, and disinfecting surfaces were introduced at a lab meeting in July. CDC guidelines were covered at the same meeting, and a self-test was administered after the presentation. The lab scrounged a Biological Safety Cabinent via Craig’s List and spent a considerable amount of time scheming ways to elevate it’s 550lbs from the floor to its stand. Solution: material lift from Home Depot.

Our Lab

The WHO Biosafety Manual was assigned to be read by all team members. We completed the CDC Interactive Training Recognizing Biosafety Levels training and quiz as a group. Our team presented real world case studies of the 2008 UCLA Lab fire death and subsequent felony OSHA prosecution, as well as the 2012 case of a VA lab worker in San Francisco who died after he was exposed to meningitis in the VA lab. Training covered PPE, instruction on our Biosafety cabinet and safe lab procedures with regard to carbon monoxide, explosive gases, fires, liquid nitrogen, and lasers. We discussed how to recognize and minimize risk by experimental design by using a real world example of how to determine the LEL of a explosive gas and minimize its danger by constructing a safe bioreactor. We have a fire extinguisher. The lab is on the 6th floor, and can be exited via elevator, stairs, and a fire escape.

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