Team:LIKA-CESAR-Brasil/TheRobot

From 2014.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 89: Line 89:
background-size:cover;
background-size:cover;
width:80%;
width:80%;
-
height:231px;
+
height:331px;
margin:auto;
margin:auto;
margin-top:40px;
margin-top:40px;
Line 99: Line 99:
background-size:cover;
background-size:cover;
width:80%;
width:80%;
-
height:231px;
+
height:331px;
margin:auto;
margin:auto;
margin-top:80px;
margin-top:80px;
Line 109: Line 109:
background-size:cover;
background-size:cover;
width:80%;
width:80%;
-
height:231px;
+
height:331px;
margin:auto;
margin:auto;
margin-top:80px;
margin-top:80px;
Line 119: Line 119:
background-size:cover;
background-size:cover;
width:80%;
width:80%;
-
height:231px;
+
height:331px;
margin:auto;
margin:auto;
margin-top:80px;
margin-top:80px;
Line 129: Line 129:
background-size:cover;
background-size:cover;
width:80%;
width:80%;
-
height:231px;
+
height:331px;
margin:auto;
margin:auto;
margin-top:80px;
margin-top:80px;
Line 139: Line 139:
background-size:cover;
background-size:cover;
width:80%;
width:80%;
-
height:231px;
+
height:331px;
margin:auto;
margin:auto;
margin-top:80px;
margin-top:80px;
Line 149: Line 149:
background-size:cover;
background-size:cover;
width:80%;
width:80%;
-
height:231px;
+
height:331px;
margin:auto;
margin:auto;
margin-top:80px;
margin-top:80px;
Line 299: Line 299:
         <li><a href="https://2014.igem.org/Team:LIKA-CESAR-Brasil/Safety">SAFETY</a></li>
         <li><a href="https://2014.igem.org/Team:LIKA-CESAR-Brasil/Safety">SAFETY</a></li>
         <li><a href="https://2014.igem.org/Team:LIKA-CESAR-Brasil/Atribuitons">ATRIBUITIONS</a></li>
         <li><a href="https://2014.igem.org/Team:LIKA-CESAR-Brasil/Atribuitons">ATRIBUITIONS</a></li>
 +
        <li class="igem-logo"><a href="https://2014.igem.org/"></a></li>
             </ul>
             </ul>
           </div><!--/.nav-collapse -->
           </div><!--/.nav-collapse -->

Latest revision as of 02:12, 18 October 2014

LIKA | CESAR

NOTEBOOK

Materials:

1.Control Unit

  • 1.Arduino UNO

2.Power Supply

  • 1.Computer Power Supply Unit (ATX)
  • 2.Custom designed plate | 2.1. 5V and 12V conectors

3.Electric Syringe

  • 1.Stteper motor
  • 2.Endless worm
  • 3.Custom designed parts
  • 4.Syringe
  • 5.IV extension tubes
  • 6.3-Way Stopcock

4.Robotic Arm

  • 1.Servo motors
  • 2.Custom designed parts

5.Centrifuge

  • 1.Brushless motor
  • 2.Esc for motor
  • 3.Centrifuge arm (custom designed)
  • 4.Motor support (custom designed)
  • 5.PCR tube support (custom designed)
  • 2.Esc for motor

1.Done manually:

  • 1.Collect a 2 mL blood sample from patient in EDTA tube with 2 mL of RNA later;
  • 2.Take a 500 μL fraction from the collected blood sample and transfer to 1.5mL PCR tubes;

2.Robot:

  • 1.Adds 1mL of lysis solution (custom designed) in the 500 μL of blood, mix and wait 10 minutes;
  • 2.After 10 minutes, heat the PCR tube at 56°C for 10 minutes;
  • 3.Centrifuge at 8.000 rpm for 5 minutes;
  • 4.Heat the PCR tube at 98ºC for 1min;
  • 1.Take 5 μL of the supernatant part of the liquid inside the PCR tube to the biosensor.
Marca LIKA Marca CESAR