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Team:EPF Lausanne/Parts
From 2014.igem.org
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486001</td> | <td class="biobrick_name">BBa_K1486001</td> | ||
| - | <td> | + | <td>Arabinose promoter + CpxR</td> |
<td>Transcription factor</td> | <td>Transcription factor</td> | ||
<td> </td> | <td> </td> | ||
| Line 164: | Line 164: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486002</td> | <td class="biobrick_name">BBa_K1486002</td> | ||
| - | <td> Arabinose promoter + sfGFP CpxR [Nterm]</td> | + | <td> Arabinose promoter + sfGFP-CpxR [Nterm]</td> |
<td>Expresses fused protein</td> | <td>Expresses fused protein</td> | ||
<td>Test CpxR expression & Ara promoter</td> | <td>Test CpxR expression & Ara promoter</td> | ||
| Line 185: | Line 185: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486005</td> | <td class="biobrick_name">BBa_K1486005</td> | ||
| - | <td> | + | <td>Arabinose promoter + sfGFP-CpxR [Cterm]</td> |
<td>Expresses fused protein</td> | <td>Expresses fused protein</td> | ||
<td>Test CpxR expression & Ara promoter</td> | <td>Test CpxR expression & Ara promoter</td> | ||
| Line 206: | Line 206: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486008</td> | <td class="biobrick_name">BBa_K1486008</td> | ||
| - | <td> | + | <td>CpxR & Split IFP1.4 [Cterm + Cterm]</td> |
<td>Two CpxR CDS, each C terminus attached to a moiety of IFP</td> | <td>Two CpxR CDS, each C terminus attached to a moiety of IFP</td> | ||
<td>Characterize CpxR dimerization</td> | <td>Characterize CpxR dimerization</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486009</td> | <td class="biobrick_name">BBa_K1486009</td> | ||
| - | <td> | + | <td>CpxR & Split IFP1.4 [Nterm + Nterm]</td> |
<td>Two CpxR CDS, each N terminus attached to a moiety of IFP</td> | <td>Two CpxR CDS, each N terminus attached to a moiety of IFP</td> | ||
<td>Characterize CpxR dimerization</td> | <td>Characterize CpxR dimerization</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486010</td> | <td class="biobrick_name">BBa_K1486010</td> | ||
| - | <td> | + | <td>CpxR & Split IFP1.4 [Nterm + Cterm]</td> |
<td>Two CpxR CDS, each attached to a moiety of IFP</td> | <td>Two CpxR CDS, each attached to a moiety of IFP</td> | ||
<td>Characterize CpxR dimerization</td> | <td>Characterize CpxR dimerization</td> | ||
| Line 227: | Line 227: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486011</td> | <td class="biobrick_name">BBa_K1486011</td> | ||
| - | <td> | + | <td>CpxR & Split IFP1.4 [Cterm + Nterm]</td> |
<td>Two CpxR CDS, each attached to a moiety of IFP</td> | <td>Two CpxR CDS, each attached to a moiety of IFP</td> | ||
<td>Characterize CpxR dimerization</td> | <td>Characterize CpxR dimerization</td> | ||
| Line 234: | Line 234: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486012</td> | <td class="biobrick_name">BBa_K1486012</td> | ||
| - | <td>CpxR-IFP[1] under pBAD | + | <td>CpxR-IFP[1] under pBAD</td> |
<td>CpxR with the Nterm moiety of IFP attached at its C terminus</td> | <td>CpxR with the Nterm moiety of IFP attached at its C terminus</td> | ||
<td>Intermediate & control</td> | <td>Intermediate & control</td> | ||
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<td class="biobrick_name">BBa_K1486026</td> | <td class="biobrick_name">BBa_K1486026</td> | ||
<td>sfGFP + Kanamycin resistance for yeast </td> | <td>sfGFP + Kanamycin resistance for yeast </td> | ||
| - | <td> | + | <td>Tag</td> |
<td>Control for the expression of Pbs2</td> | <td>Control for the expression of Pbs2</td> | ||
<td>Yeast</td> | <td>Yeast</td> | ||
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<td class="biobrick_name">BBa_K1486027</td> | <td class="biobrick_name">BBa_K1486027</td> | ||
<td>R.reniformis luciferase + ADH1 terminator + Kanamycin resistance</td> | <td>R.reniformis luciferase + ADH1 terminator + Kanamycin resistance</td> | ||
| - | <td> | + | <td>Tag</td> |
<td>Control for the expression of Pbs2 </td> | <td>Control for the expression of Pbs2 </td> | ||
<td>Yeast</td> | <td>Yeast</td> | ||
| Line 375: | Line 375: | ||
<td class="biobrick_name">BBa_K1486032</td> | <td class="biobrick_name">BBa_K1486032</td> | ||
<td>sfGFP + ADH1 terminator + Ura3 cassette</td> | <td>sfGFP + ADH1 terminator + Ura3 cassette</td> | ||
| - | <td> | + | <td>Tag</td> |
<td>Control for the expression of hog1</td> | <td>Control for the expression of hog1</td> | ||
<td>Yeast</td> | <td>Yeast</td> | ||
| Line 382: | Line 382: | ||
<td class="biobrick_name">BBa_K1486033</td> | <td class="biobrick_name">BBa_K1486033</td> | ||
<td>sfGFP + ADH1 terminator + Ura3 cassette</td> | <td>sfGFP + ADH1 terminator + Ura3 cassette</td> | ||
| - | <td> | + | <td>Tag</td> |
<td>Control for the expression of hog1</td> | <td>Control for the expression of hog1</td> | ||
<td>Yeast</td> | <td>Yeast</td> | ||
| Line 535: | Line 535: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486056</td> | <td class="biobrick_name">BBa_K1486056</td> | ||
| - | <td> | + | <td>CpxR & Split IFP1.4 [Cterm + Cterm][2]</td> |
<td>Two CpxR CDS, each C terminus attached to a moiety of the biobrick-compatible IFP</td> | <td>Two CpxR CDS, each C terminus attached to a moiety of the biobrick-compatible IFP</td> | ||
<td>Characterize CpxR dimerization</td> | <td>Characterize CpxR dimerization</td> | ||
| Line 553: | Line 553: | ||
<p class="lead">When designing the chips, the team took into account the future users and the current iGEM classification of parts. We considered it best to construct our chips as composite microfluidic parts, so their sub-parts could be used and combined in multiple ways. The flow and control layers can be separated and reused, as well as all the basic structures (chamber + connecting channel), nodes, array parts,...</p> | <p class="lead">When designing the chips, the team took into account the future users and the current iGEM classification of parts. We considered it best to construct our chips as composite microfluidic parts, so their sub-parts could be used and combined in multiple ways. The flow and control layers can be separated and reused, as well as all the basic structures (chamber + connecting channel), nodes, array parts,...</p> | ||
| - | + | <p>By clicking on each of the designs' name, you can download the original files.</p> | |
<!-- send all lines here: https://2014.igem.org/Team:EPF_Lausanne/Microfluidics/Designing --> | <!-- send all lines here: https://2014.igem.org/Team:EPF_Lausanne/Microfluidics/Designing --> | ||
<table class="table table-striped table-hover" id="chips_list"> | <table class="table table-striped table-hover" id="chips_list"> | ||
Latest revision as of 01:45, 18 October 2014
PARTS
DNA parts submitted by the 2014 EPFL iGEM team
Our team submitted a total of 55 Biobricks (part 51 does not exist).
In addition, 4 microfluidic designs have also been submitted to the registry.
| Biobrick | What it is | Function | Why do we use it? | Group |
|---|---|---|---|---|
| BBa_K1486000 | CpxR coding sequence | Transcription factor | To make most of our biobricks! | Bacteria |
| BBa_K1486001 | Arabinose promoter + CpxR | Transcription factor | Bacteria | |
| BBa_K1486002 | Arabinose promoter + sfGFP-CpxR [Nterm] | Expresses fused protein | Test CpxR expression & Ara promoter | Bacteria |
| BBa_K1486003 | Flexible linker 2x (GGGS) | Attaches two proteins together | Bacteria | |
| BBa_K1486004 | Flexible linker 2x (GGGGS) | Attaches two proteins together | Bacteria | |
| BBa_K1486005 | Arabinose promoter + sfGFP-CpxR [Cterm] | Expresses fused protein | Test CpxR expression & Ara promoter | Bacteria |
| BBa_K1486006 | IFP[1] | N terminus of split IFP | Bacteria | |
| BBa_K1486007 | IFP[2] | C terminus of split IFP | Bacteria | |
| BBa_K1486008 | CpxR & Split IFP1.4 [Cterm + Cterm] | Two CpxR CDS, each C terminus attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486009 | CpxR & Split IFP1.4 [Nterm + Nterm] | Two CpxR CDS, each N terminus attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486010 | CpxR & Split IFP1.4 [Nterm + Cterm] | Two CpxR CDS, each attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486011 | CpxR & Split IFP1.4 [Cterm + Nterm] | Two CpxR CDS, each attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486012 | CpxR-IFP[1] under pBAD | CpxR with the Nterm moiety of IFP attached at its C terminus | Intermediate & control | Bacteria |
| BBa_K1486013 | CpxR-IFP[2] under pBAD | CpxR with the Cterm moiety of IFP attached at its C terminus | Intermediate & control | Bacteria |
| BBa_K1486014 | IFP[1]-CpxR under pBAD | CpxR with the Nterm moiety of IFP attached at its N terminus | Intermediate & control | Bacteria |
| BBa_K1486015 | IFP[2]-CpxR under pBAD | CpxR with the Cterm moiety of IFP attached at its N terminus | Intermediate & control | Bacteria |
| BBa_K1486016 | fLuc[1] | N terminus moiety of the firefly luciferase | Bacteria | |
| BBa_K1486017 | fLuc[2] | C terminus moiety of the firefly luciferase | Bacteria | |
| BBa_K1486018 | Arabinose promoter + fLuc[1] + fLuc[2] | Split firefly luciferase under arabinose promoter | Control | Bacteria |
| BBa_K1486019 | rLuc[1] | C terminus moiety of the renilla luciferase | Bacteria | |
| BBa_K1486020 | rLuc[2] | N terminus moiety of the renilla luciferase | Bacteria | |
| BBa_K1486021 | Arabinose promoter + rLuc[1] + rLuc[2] | Split renilla luciferase under arabinose promoter | Control | Bacteria |
| BBa_K1486022 | Renilla Luciferase | Full renilla luciferase | Control | Bacteria |
| BBa_K1486023 | Yeast optimized superfolder GFP | Yeast optimised superfolder GFP | Reporter | Yeast |
| BBa_K1486024 | Yeast kanamycin resistance | Yeast kanamycin resistance | Selection marker | Yeast |
| BBa_K1486025 | ADH1 terminator | Terminator | Abortion of the transcription at the end of our DNA sequences | Yeast |
| BBa_K1486026 | sfGFP + Kanamycin resistance for yeast | Tag | Control for the expression of Pbs2 | Yeast |
| BBa_K1486027 | R.reniformis luciferase + ADH1 terminator + Kanamycin resistance | Tag | Control for the expression of Pbs2 | Yeast |
| BBa_K1486028 | Yeast optimized sfGFP N-terminus (1-214) | Yeast optimized sfGFP N-terminus (1-214) | Yeast | |
| BBa_K1486029 | Yeast Optimized sfGFP-N + ADH1 terminator + Kanamycin resistance | N terminal moiety of split sfGFP | Complementation Assay | Yeast |
| BBa_K1486030 | rLucN + ADH1 terminator + Kanamycin resistance | N terminal moiety of split rLuc | Complementation Assay | Yeast |
| BBa_K1486031 | CaUra3 selection marker | selection marker | Confer resistance to Uracil-deprived medium | Yeast |
| BBa_K1486032 | sfGFP + ADH1 terminator + Ura3 cassette | Tag | Control for the expression of hog1 | Yeast |
| BBa_K1486033 | sfGFP + ADH1 terminator + Ura3 cassette | Tag | Control for the expression of hog1 | Yeast |
| BBa_K1486034 | Yeast optimized superfolder GFP C-terminus (215-238) | Yeast optimized superfolder GFP C-terminus (215-238) | Yeast | |
| BBa_K1486035 | Yeast Optimized sfGFP-C + ADH1 terminator + CaUra3 Cassette | C terminal moiety of split sfGFP | Complementation Assay | Yeast |
| BBa_K1486036 | rLucC + ADH1 terminator + CaUra3 cassette | C terminal moiety of split rLuc | Complementation Assay | Yeast |
| BBa_K1486037 | 13 amino acids linker [GGGS GGGGS GGGS] | linker | Attach Pbs2 or Hog1 to our different tags and splits | Yeast |
| BBa_K1486038 | sfGFPN | N terminus moiety of split superfolder GFP | Bacteria | |
| BBa_K1486039 | sfGFPC | C terminus moiety of split superfolder GFP | Bacteria | |
| BBa_K1486040 | sfGFPN + CpxR | N terminus moiety of split sfGFP attached to CpxR | Bacteria | |
| BBa_K1486041 | sfGFPC + CpxR | C terminus moiety of split sfGFP attached to CpxR | Bacteria | |
| BBa_K1486042 | Leucine Zipper | Monomer of leucine zipper TF | Bacteria | |
| BBa_K1486043 | Leucine Zipper + split rLuc | Two Leucine Zipper monomers, each attached to a different split rLuc moiety | Control for split rLuc assays | Bacteria |
| BBa_K1486044 | IFP[1] Mutated | Biobrick-compatible IFP[1] | Bacteria | |
| BBa_K1486045 | IFP[2] Mutated | Biobrick-compatible IFP[2] | Bacteria | |
| BBa_K1486046 | CpxR promoter FW | CpxR binding-region in forward direction | Bacteria | |
| BBa_K1486047 | CpxR promoter RV | CpxR binding-region in reverse direction | Bacteria | |
| BBa_K1486048 | CpxR reporter | Calgary's CpxR reporter repaired (sequence was missing) | To see when CpxR is active | Bacteria |
| BBa_K1486049 | CpxR promoter FW + RFP | Reporter of CpxR | Test the direction of the complete CpxR promoter | Bacteria |
| BBa_K1486050 | CpxR promoter RV + RFP | Reporter of CpxR | Test the direction of the complete CpxR promoter | Bacteria |
| BBa_K1486052 | Spacer | 40 bases placed between constructs | Separate two constructs in the same plasmid | Bacteria |
| BBa_K1486053 | 10 aa linker | 10 amino-acid linker | Attach CheY/Z to split luciferases | Bacteria |
| BBa_K1486054 | CheY/CheZ + split rLuc | CheY and CheZ, each attached to a moiety of split renilla luciferase | Positive control for the split rLuc | Bacteria |
| BBa_K1486055 | CheY/CheZ + split fLuc | CheY and CheZ, each attached to a moiety of split firefly luciferase | Positive control for the split fLuc | Bacteria |
| BBa_K1486056 | CpxR & Split IFP1.4 [Cterm + Cterm][2] | Two CpxR CDS, each C terminus attached to a moiety of the biobrick-compatible IFP | Characterize CpxR dimerization | Bacteria |
Microfluidics parts (chips created)
Our team designed and made 4 microfluidic chips. At the beginning, we also used the MITOMI chip.
When designing the chips, the team took into account the future users and the current iGEM classification of parts. We considered it best to construct our chips as composite microfluidic parts, so their sub-parts could be used and combined in multiple ways. The flow and control layers can be separated and reused, as well as all the basic structures (chamber + connecting channel), nodes, array parts,...
By clicking on each of the designs' name, you can download the original files.
| Name | Main Function |
|---|---|
| SmashColi | To be able to separate the chip in 4 different compartments and apply 4 different pressures on each row of chambers. |
| The BioPad | A large and simple microfluidic chip containing 6400 chambers in which the cells are contained in. Each chamber acts as a pixel for the BioPad project. |
| CleanColi | As a result of our Safety page, we decided to create a chip that is able to seal the bacteria in the chip, preventing them to leave the chip. |
| FilterColi | To successfully immerse cells in a certain solution, this chip was designed to flow in the new medium in the chambers instead of doing it by diffusion. |
Sponsors
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