Team:HokkaidoU Japan/Notebook/H Stem/Downstream Correction

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<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li>
<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li>
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<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Estimation">Evaluation</a></li>
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<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Evaluation">Evaluation</a></li>
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      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
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Plac-stem-as-stem-dT on 1A3, found stem-dn not inserted!
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R0010-stem-as-stem-B0015(on pSB1A3) found stem-dn not inserted!
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      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
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Plac-stem-as-dT on 1A3 with XhoI-RBS(reverse) & XhoI-dT(forward)
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R0010-stem-as-B0015(on pSB1A3) with NheI_dT_F Tm:67.9&#8451; & XhoI_RBS_R Tm:68.2&#8451;
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      </ul>
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      <div class="step-recipe">
      <div class="step-recipe">
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XhoI-dT-1A3-Plac-stem-as-XhoI with XhoI
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XhoI-B0015-1A3-R0010-stem-as-XhoI with XhoI
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      </ul>
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      <div class="step-recipe">
      <div class="step-recipe">
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stem_downstream_F & stem_downstream_R
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stem_downstream_F Tm:&#8451; & stem_downstream_R
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      </ul>
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      <div class="step-recipe">
      <div class="step-recipe">
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XhoI-dT-1A3-Plac-stem-as-XhoI & stem-dn
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XhoI-B0015-1A3-R0010-stem-as-XhoI & stem-dn
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      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
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Plac-stem-as-stem-dT on 1A3
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R0010-stem-as-stem-B0015(on pSB1A3)
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  <h2 class="step-title">
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Colony PCR Sat Oct 4
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Colony PCR
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  <time>
  <time>
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Plac-stem-as-stem-dT on 1A3
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Sat Oct 4
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</li>
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      </ul>
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      <div class="step-recipe">
      <div class="step-recipe">
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Plac-stem-as-stem-dT on 1A3
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R0010-stem-as-stem-B0015(on pSB1A3) with NcoI_mRFP_F Tm:76.0&#8451; & 100_up_R Tm:62.6&#8451;
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Latest revision as of 15:28, 9 September 2015

Notebook
Lab Documents

Stem-Downstream Correction

  • Start

  • Sequencing

    R0010-stem-as-stem-B0015(on pSB1A3) found stem-dn not inserted!

  • PCR

    R0010-stem-as-B0015(on pSB1A3) with NheI_dT_F Tm:67.9℃ & XhoI_RBS_R Tm:68.2℃

  • Digestion

    XhoI-B0015-1A3-R0010-stem-as-XhoI with XhoI

  • Primer Annealing

    stem_downstream_F Tm:℃ & stem_downstream_R

  • Ligation

    XhoI-B0015-1A3-R0010-stem-as-XhoI & stem-dn

  • Transformation

    R0010-stem-as-stem-B0015(on pSB1A3)

  • Colony PCR

    R0010-stem-as-stem-B0015(on pSB1A3) with NcoI_mRFP_F Tm:76.0℃ & 100_up_R Tm:62.6℃

  • Complete!

Retrieved from "http://2014.igem.org/Team:HokkaidoU_Japan/Notebook/H_Stem/Downstream_Correction"