Team:Cambridge-JIC/Notebook

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<a href="https://2014.igem.org/Team:Cambridge-
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JIC/Notebook"style="color:#000000"> Notebook</a></td>
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<strong> Lab Notebook </strong>
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<a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Diary/2014_07_14"> 2014 07 14 - Monday </a>
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<tr><td colspan="3"> <h3>Notebook</h3></td></tr>
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== First Ideas ==
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<html> <p><br>
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<b> Mar-Cam-tia as a volatiles factory</b> <p>
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<p>Add metabolic pathways that produce perfumed volatiles (eg geraniol,
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limonene, geosmin).<p>
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Or insect repellents/attractants such as E-beta-farnesene to repel aphids and
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attract ladybirds, or bombykol to attract moths from miles away.<p>
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Production could be linked to circadian clock to change smell (or species of
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moth attracted) throughout the day -<b> ie Mar-Cam-tia as a clock.</b><p>
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There could be a link to Marchy's air pores.<p>
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<br>
<br>
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<b>Mar-Cam-tia as a new form of LCD screen</b><p>
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<div align="center">
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If chromoprotein expression can be triggered in arbitrary cells by irradiating
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<a href="https://2014.igem.org/Special:Upload">Use this page to upload pictures to the wiki</a>
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them with a far-red laser to induce the phytochrome signalling pathway in
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them, thalli could be written on with light and an image formed. Different
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lasers, different colour chromoproteins = full palette of laser-plant-pens.<p>
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<br>
<br>
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<b>Mar-Cam-tia as phytoELISA</b><p>
 
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An antigen receptor based on an antibody would be expressed on the surface of
 
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Marchantia, and a potentially novel signalling pathway (perhaps involving cAMP
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<div id="lab notebook" align="center">
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Lab notebook:
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_1">Week 1</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_2">Week 2</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_3">Week 3</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_4">Week 4</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_5">Week 5</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_6">Week 6</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_7">Week 7</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_8">Week 8</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_9">Week 9</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Notebook/Lab_Week_10">Week 10</a></li>
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</ul>
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</div><br/>
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production or a tyrosine kinase dimer formation mechanism) engineered such
 
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that when the antigen of interest binds with the receptor, downstream events
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<div id="constructs" align="center">
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Constructs:
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Constructs/progress_template">Construct Progress Template</li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Constructs/gal4_hap1gr_H2RBmRFP">Gal4-Hap1GR-H2RBmRFP</a></li>
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<li><a href="https://2014.igem.org/Team:Cambridge-JIC/Constructs/35S-HBRmRFP">35S-HBRmRFP</a></li>
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</ul>
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</div><br>
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induce an obvious reporting process such as chromoprotein expression,
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luciferin-mediated glowing, morphological changes or the release of a
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characteristic smell. Qualitative disease testing with a product that
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replicates itself out of water and sunshine instead of a single use ELISA that
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costs $300.<p>
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<br>
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<b>Mar-Cam-tia as a reading lamp</b><p>
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Engineer the luciferin-luciferase system that the 2010 Cambridge team put into
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'E. glowli' into Marchy, and show the dodgy kickstarter dude how it should
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really be done. This could be linked to the plant's circadian rhythm to ensure
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only nightime glowing.<p>
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<br>
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<b>Marchantia change-o-morpha</b><p>
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A morphological study of Marchy: Can we engineer shape? Can we disrupt the
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normal course of growth and shape formation? Maybe we can shape it like a
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cauliflower.<p>
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<br>
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<b>Spore removal</b><p>
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As is, spores are a biocontainment issue. If Marchy was less sexually prolific
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we would have less of a problem. It would be interesting and aesthetically
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pleasing to turn the gametophores into the readout of a potential biosensor.
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Latest revision as of 15:47, 30 July 2014