Team:SCU-China/Safety

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   <div style="background-image: url(https://static.igem.org/mediawiki/2014/thumb/1/1c/ScuSafety.png/382px-ScuSafety.png); background-size: 160px auto; background-repeat: no-repeat; background-clip: border-box; background-position: 100% 30%;padding-top: 40px; padding-bottom: 40px;"><p style="font-size:100px;">PCR Protocol </P></div>
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   <div style="background-image: url(https://static.igem.org/mediawiki/2014/thumb/1/1c/ScuSafety.png/382px-ScuSafety.png); background-size: 160px auto; background-repeat: no-repeat; background-clip: border-box; background-position: 100% 30%;padding-top: 40px; padding-bottom: 40px;"><p style="font-size:100px;">Safety </P></div>
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               <li ><a href="#Top">Back to top</a></li>
               <li ><a href="#Top">Back to top</a></li>
   
   
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</ul> </div>
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<li ><a href="#1">1. Your Training</a></li>
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<li ><a href="#2">2. Your Local Rules and Regulations</a></li>
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<li ><a href="#3">4. Risks of Your Project Now</a></li>
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<li ><a href="#4">5. Risks of Your Project in the Future</a></li>
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<li ><a href="#5">6. Further Comments</a></li></ul> </div>
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<h1>1. Your Training</h1>
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<h1 id="1">1. Your Training</h1>
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    <h3 class="panel-title">Have your team members received any safety training yet?</h3>
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    Yes, we have already received safety training.
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  <div class="panel-heading">
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    <h3 class="panel-title">Please briefly describe the topics that you learned about (or will learn about) in your safety training.</h3>
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    The advisors have given us the safety training such as what risks the chemicals may cause and how to avoid them or reduce the harm as much as possible before the experiment .
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  </div>
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</div>
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<h3 >Have your team members received any safety training yet?</h3>
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<p>Yes, we have already received safety training.</p>
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  <div class="panel-heading">
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    <h3 class="panel-title">Please give a link to the laboratory safety training requirements of your institution (college, university, community lab, etc). Or, if you cannot give a link, briefly describe the requirements.</h3>
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    Yes, our school has a special department responsible for this part, their officers have inspected our laboratory and gave us suggestions about biosafety.
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<h3> Please briefly describe the topics that you learned about (or will learn about) in your safety training.</h3>
 
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<p>The advisors have given us the safety training such as what risks the chemicals may cause and how to avoid them or reduce the harm as much as possible before the experiment .</p>
 
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<h1 id="2">2. Your Local Rules and Regulations</h1>
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<div class="panel panel-default">
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  <div class="panel-heading">
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    <h3 class="panel-title">Who is responsible for biological safety at your institution? (You might have an Institutional Biosafety Committee, an Office of Environmental Health and Safety, a single Biosafety Officer, or some other arrangement.) Have you discussed your project with them? Describe any concerns they raised, and any changes you made in your project based on your discussion.</h3>
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    School of Life Sciences in Sichuan University.and Mr. Yang is the lab. manager of our iGEM team.
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  </div>
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<h3> Please give a link to the laboratory safety training requirements of your institution (college, university, community lab, etc). Or, if you cannot give a link, briefly describe the requirements.</h3>
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<p>Yes, our school has a special department responsible for this part, their officers have inspected our laboratory and gave us suggestions about biosafety.</p>
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    <h3 class="panel-title">What are the biosafety guidelines of your institution? Please give a link to these guidelines, or briefly describe them if you cannot give a link.</h3>
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    http://labsb.scu.edu.cn/sbc/detail.jsp?portalId=723&cid=8125&nextcid=8142&againNextcid=8207&aid=46365
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</div></div>
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<div class="panel panel-default">
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  <div class="panel-heading">
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    <h3 class="panel-title">In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</h3>
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  </div>
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  <div class="panel-body">
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    http://www.lascn.net/policy/law/nationlaw/201212/6374.html
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<h1 id="3">4. Risks of Your Project Now</h1>
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<legend class="qnum">2. Your Local Rules and Regulations</legend>
 
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<p class="qbody"><span class="qnum">a)</span> Who is responsible for biological safety at your institution? (You might have an Institutional Biosafety Committee, an Office of Environmental Health and Safety, a single Biosafety Officer, or some other arrangement.) Have you discussed your project with them? Describe any concerns they raised, and any changes you made in your project based on your discussion.</p>
 
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<br>
 
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<p class="qbody"><span class="qnum">b)</span> What are the biosafety guidelines of your institution? Please give a link to these guidelines, or briefly describe them if you cannot give a link.</p>
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<h3>Please describe risks of working with the biological materials (cells, organisms, DNA, etc.) that you are using in your project. If you are taking any safety precautions (even basic ones, like rubber gloves), that is because your work has some risks, however small. Therefore, please discuss possible risks and what you have done (or might do) to minimize them, instead of simply saying that there are no risks at all.</h3>
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<div class="wrapper" style="background-color: white;"><textarea rows="2" id="inst_biosafety_guidelines" data-form-field="inst_biosafety_guidelines" disabled=""></textarea></div>
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  <div class="panel-heading">
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    <h3 class="panel-title"> Risks to the safety and health of team members, or other people working in the lab:</h3>
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    The engineered strains used in this project are the most commonly used E. coli DH5α, TOP10 and BL21 which cause no severe infection to human and animals and would be inactivated after experiments. The chemicals frequently used are proved to be friendly and will be handled properly by well-trained students.
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</div></div>
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<p class="qbody"><span class="qnum">c)</span> In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</p>
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<div class="wrapper" style="background-color: white;"><textarea rows="2" id="national_biosafety_rules" data-form-field="national_biosafety_rules" disabled=""></textarea></div>
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    <h3 class="panel-title"> Risks to the safety and health of the general public (if any biological materials escaped from your lab):</h3>
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  Since our experiment design is just about the common E.coli and the parts we used or modified are mostly reporters and regulator devices which have been used by former iGEM teams and proved to be innocuous. Even if they are released by accident, they won't cause any severe infection.
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<legend class="qnum">3. The Organisms and Parts that You Use</legend>
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<p>Please <a href="https://2014.igem.org/File:TeamName_Safety2014_Spreadsheet.xls" target="_blank">visit this page</a> to download a blank copy of the spreadsheet for question 3. (If you need a CSV version instead of XLS, <a href="https://2014.igem.org/File:TeamName_Safety2014_CSV.txt" target="_blank">visit this page</a>.)</p>
 
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<p>Complete the spreadsheet. Include all whole organisms that you will handle in the lab, whether you are using them as a chassis or for some other reason. Include all <strong>new</strong> or <strong>highly modified</strong> protein coding parts that you are using. If you submitted a Check-In for an organism or part, you should still include it in this spreadsheet.</p>
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    <h3 class="panel-title">  Risks to the environment (from waste disposal, or from materials escaping from your lab):</h3>
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  </div>
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  <div class="panel-body">
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  The E.coli strains are widely used, so we believe they won't have much impact on the environment. Actually, we have inactivated all the strains strictly after experiments.
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<p>You may omit non-protein-coding parts, and you may omit parts that were already in the Registry if you are using them without significant modifications.</p>
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     <div><button type="button" id="toggle_spreadsheet_instructions">Click here to show/hide instructions for completing the spreadsheet</button></div>
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     <h3 class="panel-title">   Risks to security through malicious mis-use by individuals, groups, or countries:</h3>
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        <p><strong>Remember to change the filename of your spreadsheet! Put your team's name in place of "TeamName".</strong></p>
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  No, since all the engineered strains are frequently used by researchers all over the world and mostly exist in laboratory environments.  
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            <li><strong>Species name (including strain):</strong> For an organism, give the scientific name of the species. Include a strain name or number (such as "K-12" for E. coli K-12) if there is one. For a part, give the name and strain of the organism that the part originally came from.</li>
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</div></div>
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            <li><strong>Risk Group:</strong> Give the Risk Group of the organism in column A. You may use a categorization according to your home country, according to the USA, or according to the WHO. If the organism falls into an 'in-between' or special category such as 2+ or 2-Agricultural, explain this category in the Notes column. If you cannot find any Risk Group categorization for this organism, write "N/A" and explain in the Notes column. (Multicellular organisms generally do not have a Risk Group.)</li>
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<div class="panel panel-default">
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            <li><strong>Risk Group Source:</strong> Cite the source from which you obtained the Risk Group information. See <a href="https://2014.igem.org/Safety/Risk_Group_Guide#rghowto">Risk Group Guide</a> for recommended sources. If you got the information from the Canadian PSDS, from the NIH Guidelines, or from the DSMZ catalogue, you may simply write "PSDS", "NIH", or "DSMZ". Otherwise, please give a web link or a full citation for your source.</li>
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  <div class="panel-heading">
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            <li><strong>Disease risk to humans?:</strong> Does this organism cause any disease in humans? If yes, what disease does it cause?</li>
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    <h3 class="panel-title"> What measures are you taking to reduce these risks? (For example: safe lab practices, choices of which organisms to use.)</h3>
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            <li><strong>Part number/name:</strong> For a part: If it has a Registry part number (like BBa_XXXXX), write that number. If it has no Registry part number, give a short name for the part. (For example: "Actin", "Alcohol Dehydrogenase".) For a whole organism, leave this column blank.</li>
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  </div>
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            <li><strong>Natural function of part:</strong> For a part: Briefly describe what the part does in its parent organism. (If it is an enzyme, what reaction does it catalyze? If it is a receptor, what molecules does it bind to? Etc.) For a whole organism, leave this column blank.</li>
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            <li><strong>How did you acquire it?:</strong> Describe how you acquired the organism/part. If you have not acquired it yet, describe how you plan to acquire it. (For example: did you receive the part DNA from another lab? Did you order the part DNA from a synthesis company? Did you use PCR to isolate the part from genomic DNA of its parent organism? Did you order the cell line from a company?)</li>
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All materials that touch our engineering bacteria are sterilized and all the bacteria are killed strictly. In the meantime, we ask all of our team members to take care of their own safety especially when they do the experiments with some deleterious compounds such as EB.
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            <li><strong>How will you use it?:</strong> Describe how you are using the organism/part in the lab. (For example: "This organism is our chassis." "This part senses when the cells are exposed to glucose." "This organism is the source for a part that we are isolating by PCR." "This part produces the toxin which our bio-sensor is designed to detect.")</li>
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            <li><strong>Notes:</strong> Use this column to give any additional information that is necessary.</li>
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        </ol>
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<p><strong><a href="https://2014.igem.org/wiki/index.php?title=Special:Upload&amp;wpDestFile=SCU-China_Safety2014_Spreadsheet.xls" target="_blank" id="spreadsheet_upload"><button type="button">Upload Spreadsheet</button></a> -- Please do not change the "Destination Filename"!</strong></p>
 
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<p>You may upload multiple versions of your spreadsheet, using the same Destination Filename. The wiki software will keep track of different versions, and list them in chronological order.</p>
 
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<p><strong><a href="https://2014.igem.org/File:SCU-China_Safety2014_Spreadsheet.xls" target="_blank" id="spreadsheet_view">Click here to VIEW your spreadsheet</a></strong></p>
 
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<legend class="qnum">4. Risks of Your Project Now</legend>
 
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<h1 id="4">5. Risks of Your Project in the Future<h1>
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<p class="qbody">Please describe risks of working with the biological materials (cells, organisms, DNA, etc.) that you are using in your project. If you are taking any safety precautions (even basic ones, like rubber gloves), that is because your work has some risks, however small. Therefore, please discuss possible risks and what you have done (or might do) to minimize them, instead of simply saying that there are no risks at all.</p>
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<br>
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<h3>What would happen if all your dreams came true, and your project grew from a small lab study into a commercial/industrial/medical product that was used by many people? We invite you to speculate broadly and discuss possibilities, rather than providing definite answers. Even if the product is "safe", please discuss possible risks and how they could be addressed, rather than simply saying that there are no risks at all.</h3>
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<p class="qbody"><span class="qnum">a)</span> Risks to the safety and health of team members, or other people working in the lab:</p>
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<div class="wrapper" style="background-color: white;"><textarea rows="4" id="lab_worker_risks_now" data-form-field="lab_worker_risks_now" disabled=""></textarea></div>
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  <div class="panel-heading">
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    <h3 class="panel-title">What new risks might arise from your project's growth? (Consider the categories of risk listed in parts a-d of the previous question: lab workers, the general public, the environment, and malicious mis-uses.) Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</h3>
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In our project, all the bacteria we used are harmless bacteria and all the plasmids are harmless too, though, but we need to culture those bacteria together. As a result, we must concede there is a little possibility producing some harmful materials because of the gene communications.
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<p class="qbody"><span class="qnum">b)</span> Risks to the safety and health of the general public (if any biological materials escaped from your lab):</p>
 
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<div class="wrapper" style="background-color: white;"><textarea rows="4" id="public_risks_now" data-form-field="public_risks_now" disabled=""></textarea></div>
 
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<p class="qbody"><span class="qnum">c)</span> Risks to the environment (from waste disposal, or from materials escaping from your lab):</p>
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  <div class="panel-heading">
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    <h3 class="panel-title"> Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks? (For example: auxotrophic chassis, physical containment, etc.) Such features are not required for an iGEM project, but many teams choose to explore them.</h3>
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One of the applications of our project is to produce some useful products via the complex gene pathways. And the first thing that we should take into consideration is the side products especially these deleterious side products. Under this suituation, we suggest that we should keep our gene pathways as simple as possible.
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<p class="qbody"><span class="qnum">d)</span> Risks to security through malicious mis-use by individuals, groups, or countries:</p>
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<h1 id="5">6. Further Comments</h1>
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<div class="wrapper" style="background-color: white;"><textarea rows="4" id="security_now" data-form-field="security_now" disabled=""></textarea></div>
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<h3>If you are completing a Preliminary Version of your Safety Form, use this space to describe how far you have progressed in your project, and give some comments about any questions that you left blank.</h3>
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    <h3 class="panel-title">You can also use this space for any other comments or additional material.</h3>
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According to my consideration, we carried out the safety form extremely strictly in the parts about the safety of our team members and our lab because the most precious thing for everyone is their life. However, in the meanwhile, we also cannot deny that we did something not very well in the parts about the potential problems of our project because we don't and we cannot take everything into consideration especially for the application of our project in that there is still a pretty long way to go. But, The only thing that I can say without any hesitation is that we did the BEST that we CAN.
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<p class="qbody"><span class="qnum">e)</span> What measures are you taking to reduce these risks? (For example: safe lab practices, choices of which organisms to use.)</p>
 
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<legend class="qnum">5. Risks of Your Project in the Future</legend>
 
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<p><strong>What would happen if all your dreams came true, and your project grew from a small lab study into a commercial/industrial/medical product that was used by many people? We invite you to speculate broadly and discuss possibilities, rather than providing definite answers. Even if the product is "safe", please discuss possible risks and how they could be addressed, rather than simply saying that there are no risks at all.</strong></p>
 
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<p class="qbody"><span class="qnum">a)</span> What <i>new</i> risks might arise from your project's growth? (Consider the categories of risk listed in parts a-d of the previous question: lab workers, the general public, the environment, and malicious mis-uses.) Also, what risks might arise if the <i>knowledge</i> you generate or the <i>methods</i> you develop became widely available?</p>
 
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<p class="qbody"><span class="qnum">b)</span> Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks? (For example: auxotrophic chassis, physical containment, etc.) Such features are not required for an iGEM project, but many teams choose to explore them.</p>
 
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<legend class="qnum">6. Further Comments</legend>
 
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<p class="qbody">If you are completing a Preliminary Version of your Safety Form, use this space to describe how far you have progressed in your project, and give some comments about any questions that you left blank.</p>
 
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<legend><h4>Submit</h4></legend>
 
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<p>Only a team Instructor (or Primary Contact) may submit the Safety Form.</p>
 
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<p>Instructors, please read the form you are submitting, and confirm that all its information is correct. By checking the "I Agree" box and clicking the "Submit" button, you are agreeing that the Safety Form accurately describes the activities of your team. We are using the "I Agree" box in lieu of a signature with paper and pen.</p>
 
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Latest revision as of 21:32, 17 October 2014

Safety

1. Your Training

Have your team members received any safety training yet?

Yes, we have already received safety training.

Please briefly describe the topics that you learned about (or will learn about) in your safety training.

The advisors have given us the safety training such as what risks the chemicals may cause and how to avoid them or reduce the harm as much as possible before the experiment .

Please give a link to the laboratory safety training requirements of your institution (college, university, community lab, etc). Or, if you cannot give a link, briefly describe the requirements.

Yes, our school has a special department responsible for this part, their officers have inspected our laboratory and gave us suggestions about biosafety.

2. Your Local Rules and Regulations

Who is responsible for biological safety at your institution? (You might have an Institutional Biosafety Committee, an Office of Environmental Health and Safety, a single Biosafety Officer, or some other arrangement.) Have you discussed your project with them? Describe any concerns they raised, and any changes you made in your project based on your discussion.

School of Life Sciences in Sichuan University.and Mr. Yang is the lab. manager of our iGEM team.

What are the biosafety guidelines of your institution? Please give a link to these guidelines, or briefly describe them if you cannot give a link.

http://labsb.scu.edu.cn/sbc/detail.jsp?portalId=723&cid=8125&nextcid=8142&againNextcid=8207&aid=46365

In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.

http://www.lascn.net/policy/law/nationlaw/201212/6374.html

4. Risks of Your Project Now

Please describe risks of working with the biological materials (cells, organisms, DNA, etc.) that you are using in your project. If you are taking any safety precautions (even basic ones, like rubber gloves), that is because your work has some risks, however small. Therefore, please discuss possible risks and what you have done (or might do) to minimize them, instead of simply saying that there are no risks at all.

Risks to the safety and health of team members, or other people working in the lab:

The engineered strains used in this project are the most commonly used E. coli DH5α, TOP10 and BL21 which cause no severe infection to human and animals and would be inactivated after experiments. The chemicals frequently used are proved to be friendly and will be handled properly by well-trained students.

Risks to the safety and health of the general public (if any biological materials escaped from your lab):

Since our experiment design is just about the common E.coli and the parts we used or modified are mostly reporters and regulator devices which have been used by former iGEM teams and proved to be innocuous. Even if they are released by accident, they won't cause any severe infection.

Risks to the environment (from waste disposal, or from materials escaping from your lab):

The E.coli strains are widely used, so we believe they won't have much impact on the environment. Actually, we have inactivated all the strains strictly after experiments.

Risks to security through malicious mis-use by individuals, groups, or countries:

No, since all the engineered strains are frequently used by researchers all over the world and mostly exist in laboratory environments.

What measures are you taking to reduce these risks? (For example: safe lab practices, choices of which organisms to use.)

All materials that touch our engineering bacteria are sterilized and all the bacteria are killed strictly. In the meantime, we ask all of our team members to take care of their own safety especially when they do the experiments with some deleterious compounds such as EB.

5. Risks of Your Project in the Future

What would happen if all your dreams came true, and your project grew from a small lab study into a commercial/industrial/medical product that was used by many people? We invite you to speculate broadly and discuss possibilities, rather than providing definite answers. Even if the product is "safe", please discuss possible risks and how they could be addressed, rather than simply saying that there are no risks at all.

What new risks might arise from your project's growth? (Consider the categories of risk listed in parts a-d of the previous question: lab workers, the general public, the environment, and malicious mis-uses.) Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?

In our project, all the bacteria we used are harmless bacteria and all the plasmids are harmless too, though, but we need to culture those bacteria together. As a result, we must concede there is a little possibility producing some harmful materials because of the gene communications.

Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks? (For example: auxotrophic chassis, physical containment, etc.) Such features are not required for an iGEM project, but many teams choose to explore them.

One of the applications of our project is to produce some useful products via the complex gene pathways. And the first thing that we should take into consideration is the side products especially these deleterious side products. Under this suituation, we suggest that we should keep our gene pathways as simple as possible.

6. Further Comments

If you are completing a Preliminary Version of your Safety Form, use this space to describe how far you have progressed in your project, and give some comments about any questions that you left blank.

You can also use this space for any other comments or additional material.

According to my consideration, we carried out the safety form extremely strictly in the parts about the safety of our team members and our lab because the most precious thing for everyone is their life. However, in the meanwhile, we also cannot deny that we did something not very well in the parts about the potential problems of our project because we don't and we cannot take everything into consideration especially for the application of our project in that there is still a pretty long way to go. But, The only thing that I can say without any hesitation is that we did the BEST that we CAN.

Sichuan university

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