Team:Hong Kong HKUST/riboregulator/parts

From 2014.igem.org

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<div class = "catalog_table_area">
<div class = "catalog_table_area">
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<div class='content_2'><h3><font color="white">Parts that we designed and submitted</font></h3></div>
 
<table class= "catalog_table">
<table class= "catalog_table">
<thead>
<thead>
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  <td>Intermediate</td>       
  <td>Intermediate</td>       
  <td>Lock 3-GFP-B0015</td>    
  <td>Lock 3-GFP-B0015</td>    
-
  <td>905</td>
+
  <td>903</td>
</tr>
</tr>
 +
<tr>
<tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379023">BBa_K1379023</a></td>
 +
  <td>Intermediate</td>     
 +
  <td>Key 3-B0015</td>  
 +
  <td>231</td>
 +
</tr>
 +
 +
                                <tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379024">BBa_K1379024</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379024">BBa_K1379024</a></td>  
  <td>Intermediate</td>       
  <td>Intermediate</td>       
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</tr>
</tr>
 +
 +
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379026">BBa_K1379026</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379026">BBa_K1379026</a></td>  
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</tr>  
</tr>  
 +
 +
                                <tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379027">BBa_K1379027</a></td>
 +
  <td>Intermediate</td>     
 +
  <td>K175032-B0015</td>  
 +
  <td>221</td>
 +
</tr>
 +
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379028">BBa_K1379028</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379028">BBa_K1379028</a></td>  
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</tr>  
</tr>  
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379046">BBa_K1379046</a></td>
 
-
  <td>Intermediate</td>     
 
-
  <td>RBS(Lock 1)-GFP-B0015</td>  
 
-
  <td>873</td>
 
-
</tr>
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379047">BBa_K1379047</a></td>
 
-
  <td>Intermediate</td>     
 
-
  <td>RBS(Lock 3c)-GFP-B0015</td>  
 
-
  <td>875</td>
 
-
</tr>
 
-
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379048">BBa_K1379048</a></td>
 
-
  <td>Measurement</td>     
 
-
  <td>J23102-Lock 1-GFP-B0015-pBad/araC-Key 1-B0015</td>  
 
-
  <td>2403</td>
 
-
</tr>
 
-
 
-
</table>
 
-
 
-
<br><br>
 
-
<div class='content_2'><h3><font color="white">Parts that we constructed and submitted</font></h3></div>
 
-
<table class= "catalog_table">
 
-
<thead>
 
-
<tr>
 
-
<th scope="col">Name</th>
 
-
<th scope="col">Type</th>
 
-
<th scope="col">Description</th>
 
-
<th scope="col">Length</th>
 
-
</tr>
 
-
</thead>
 
-
<tbody>
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_I0500">BBa_I0500</a></td>
 
-
  <td>Inducible pBad/araC promoter</td>     
 
-
  <td>Inducible pBad/araC promoter</td>  
 
-
  <td>1210</td>
 
-
</tr>
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01010">BBa_J01010</a></td>
 
-
  <td>Riboregulator Lock 1</td>     
 
-
  <td>Biobrick version of Isaacs' riboregulator cis repressed lock, crR12.</td>  
 
-
  <td>40</td>
 
-
</tr>
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01080">BBa_J01080</a></td>
 
-
  <td>Lock3</td>     
 
-
  <td>Biobrick version of Isaacs' riboregulator cis repressed lock, crR12, and modified version of Riboregulator Lock 1.</td>  
 
-
  <td>40</td>
 
-
</tr>
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01086">BBa_J01086</a></td>
 
-
  <td>Key3</td>     
 
-
  <td>Biobricked version of Isaacs' riboregulator trans activating key, taR12, and modified version of Riboregulator Key 1.</td>  
 
-
  <td>94</td>
 
-
</tr>
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K175031">BBa_K175031</a></td>
 
-
  <td>Lock for medium RBS (B0032) from the lock/key library TUD09</td>     
 
-
  <td>This biobrick generates a mRNA which forms a secondary structure blocking the (medium) RBS.</td>  
 
-
  <td>47</td>
 
-
</tr>
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K175032">BBa_K175032</a></td>
 
-
  <td>Key for lock of medium (K175031) RBS (B0032) from the lock/key library TUD09</td>     
 
-
  <td>This biobrick generates a mRNA which forms a secondary structure that opens LMR (K175031).</td>  
 
-
  <td>84</td>
 
-
</tr>
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J23080">BBa_J23080</a></td>
 
-
  <td>B0034 derived RBS to match spacing of J01122</td>     
 
-
  <td>RBS spacing modification to [RBS] such that the spacing between the shine-delgarno and the ORF is the same as it is in J01122.</td>  
 
-
  <td>10</td>
 
-
</tr>
 
-
 
-
 
-
</tbody>
 
-
</table>
 
-
 
-
<br><br>
 
-
<div class='content_2'><h3><font color="white">Parts that already exist in Parts Registry and are currently under characterization</font></h3></div>
 
-
<table class= "catalog_table">
 
-
<thead>
 
-
<tr>
 
-
<th scope="col">Name</th>
 
-
<th scope="col">Type</th>
 
-
<th scope="col">Description</th>
 
-
<th scope="col">Length</th>
 
-
</tr>
 
-
</thead>
 
-
<tbody>
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01008">BBa_J01008</a></td>
 
-
  <td>Riboregulator Lock 1</td>     
 
-
  <td>Lock 1</td>  
 
-
  <td>94</td>
 
-
</tr>
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J23031">BBa_J23031</a></td>
 
-
  <td>Lock 3c</td>     
 
-
  <td>lock3c</td>  
 
-
  <td>42</td>
 
-
</tr>
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J23008">BBa_J23008</a></td>
 
-
  <td>Key 3c</td>     
 
-
  <td>Encodes a short RNA sequence that "unlocks" the biobrick part J01122 (lock3) which is locked RFP.</td>  
 
-
  <td>42</td>
 
-
</tr>
 
-
 
-
</tbody>
 
-
</table>
 
-
 
-
<br><br>
 
-
<div class = "catalog_table_area">
 
-
<div class='content_2'><h3><font color="white">Parts that we designed but did not submit</font></h3></div>
 
-
<table class= "catalog_table">
 
-
<thead>
 
-
<tr>
 
-
<th scope="col">Name</th>
 
-
<th scope="col">Type</th>
 
-
<th scope="col">Description</th>
 
-
<th scope="col">Length</th>
 
-
</tr>
 
-
</thead>
 
-
<tbody>
 
-
 
-
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379021">BBa_K1379021</a></td>
 
-
  <td>Intermediate</td>     
 
-
  <td>Key 1-B0015</td>  
 
-
  <td>231</td>
 
-
</tr>
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379023">BBa_K1379023</a></td>
 
-
  <td>Intermediate</td>     
 
-
  <td>Key 3-B0015</td>  
 
-
  <td>231</td>
 
-
</tr>
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379025">BBa_K1379025</a></td>
 
-
  <td>Intermediate</td>     
 
-
  <td>Key 3c-B0015</td>  
 
-
  <td>231</td>
 
-
</tr>
 
-
 
-
                                <tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379027">BBa_K1379027</a></td>
 
-
  <td>Intermediate</td>     
 
-
  <td>K175032-B0015</td>  
 
-
  <td>221</td>
 
-
</tr>
 
-
 
                                 <tr>
                                 <tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379029">BBa_K1379029</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379029">BBa_K1379029</a></td>  
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</tr>
</tr>
-
                                <tr>
+
                             
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379031">BBa_K1379031</a></td>
+
-
  <td>Intermediate</td>     
+
-
  <td>pBad/araC-Key 1</td>  
+
-
  <td>1312</td>
+
-
</tr>
+
                                 <tr>
                                 <tr>
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  <td>pBad/araC-HKUST Key 1</td>    
  <td>pBad/araC-HKUST Key 1</td>    
  <td>1300</td>
  <td>1300</td>
-
</tr>
+
</tr>                         
 +
 
 +
 +
<tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379046">BBa_K1379046</a></td>
 +
  <td>Intermediate</td>     
 +
  <td>RBS(Lock 1)-GFP-B0015</td>  
 +
  <td>873</td>
 +
</tr>
 +
 +
<tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379047">BBa_K1379047</a></td>
 +
  <td>Intermediate</td>     
 +
  <td>RBS(Lock 3c)-GFP-B0015</td>  
 +
  <td>875</td>
 +
</tr>  
-
                         
+
 
-
                             
+
<tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379048">BBa_K1379048</a></td>
 +
  <td>Measurement</td>     
 +
  <td>J23102-Lock 1-GFP-B0015-pBad/araC-Key 1-B0015</td>  
 +
  <td>2403</td>
 +
</tr>
</table>
</table>
 +
</div>
</div>

Latest revision as of 22:06, 17 October 2014



Riboregulator Parts

Name Type Description Length
BBa_K1379015 RBS RBS(Lock 3c) 12
BBa_K1379018 RNA HKUST Lock 1 45
BBa_K1379019 RNA HKUST Key 1 82
BBa_K1379020 Intermediate Lock 1-GFP-B0015 903
BBa_K1379022 Intermediate Lock 3-GFP-B0015 903
BBa_K1379023 Intermediate Key 3-B0015 231
BBa_K1379024 Intermediate Lock 3c-GFP-B0015 905
BBa_K1379026 Intermediate K175031-GFP-B0015 910
BBa_K1379027 Intermediate K175032-B0015 221
BBa_K1379028 Intermediate HKUST Lock 1-GFP-B0015 908
BBa_K1379029 Intermediate HKUST Key 1-B0015 219
BBa_K1379030 Measurement J23102-Lock 1-GFP-B0015 946
BBa_K1379032 Measurement J23102-Lock 3-GFP-B0015 948
BBa_K1379033 Intermediate pBad/araC-Key 3 1312
BBa_K1379034 Measurement J23102-Lock 3c-GFP-B0015 948
BBa_K1379035 Intermediate pBad/araC-Key 3c 1312
BBa_K1379036 Measurement J23102-K175031-GFP-B0015 953
BBa_K1379037 Intermediate pBad/araC-K175032 1302
BBa_K1379038 Measurement J23102-HKUST Lock 1-GFP-B0015 951
BBa_K1379039 Intermediate pBad/araC-HKUST Key 1 1300
BBa_K1379046 Intermediate RBS(Lock 1)-GFP-B0015 873
BBa_K1379047 Intermediate RBS(Lock 3c)-GFP-B0015 875
BBa_K1379048 Measurement J23102-Lock 1-GFP-B0015-pBad/araC-Key 1-B0015 2403

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