Team:SCU-China/Biobricks

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<p>Finally we have to submit some biobricks that we made in our experiment. This notebook is focusing on constructing independent biobricks.There are about 30 biobricks that we plan to submit. In detail, we separate the into 4 groups:</p><p>
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Group one contains 8 different parts (Am4: Pcon+araC+pBAD; Am5: pRhl+RBS+mRFP+DT; Am6: pRHl/Las+RBS+GFP+DT; Am7: Pcon+RBS+LasR; Am8: RhlR+DT; Am9: RBS+amilCP+DT; Am10:RBS+amilCP+DT+Pcon+RBS; Am11: pRhl/Las+RBS); </p><p>
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Group two contains 8 different parts (Bm1: RBS+LasR+RhlR+DT; Bm2: Ptet+RBS+RhlR+DT; Bm3: Pcon+RBS_LasR+RhlR+DT; Bm4: pCin+CinR; Bm5: Pcon+RBS+Lac I; Bm6: pLac+RBS+luxI+DT; Bm7: RBS+CinI+DT; Bm8: pLux+RBS+LuxR+RBS+RhlI)</p>
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   margin-top: -45px;"><h1>Week 1 8.21-8.24</h1>
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<h1>Brainstorm</h1>
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<p>1.We resuscitated the bacteria that contain Am4-Am11 parts.</p><p>
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2.We extracted all the plasmids and tested them by electrophoresis.</p><p>
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<p>From last winter holiday, we started our brainstorm which all the team members were involved in. During this period, we shared our ideas, thoughts, and designs with each other and In May, we decided our project. In the following months we finished our design of our proect.</P>
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3.All the plasmids were cleaved by EcoR I and Pst I and we tested their qualities by electrophoresis.</p><p>
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4.We linked all the cleaved parts with pSB1C3 after we did the gel purification.</p><p>
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5.We transformed all the parts into E.coli. and extracted the plasmids which were tested by double cleavage.</p><p>
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6.We sent all the parts to sequence.</p></div>
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<p>The wetlab work mainly contains the designing project, selecting genes, and other experiments like cloning, transforming. Almost all the team members participate in these work.</p>
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<p>There are 3 persons Wang Nan, Liang Fengyan, and Fang Lin in charge of our modeling work. They come from School of Life Sciences, Chemistry, and Mathematics, respectively.</p>
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<p>Xie Weize is responsible for constructing our Wiki website. In the meanwhile, Liang Fengyan, Ron Li, and Zhang Zhilong also participate in this work. The 4 persons instruct us to design and decorate the website and also other members give some favors to them.</p>
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<p>Subjects on safety were discussed among the SCU iGEM team. Safety form was finished by Zheng Xu.</p>
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<p>Ron Li helps us finish our poster and in the meantime, she is also in charge of giving some advice about the design and decorating. In detail, she draw a whole lot of pictures and signs in our website and Powerpoint. Also, Wang Nan and Zhou Yu give her a great lot of help.</p>
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Week 2 8.25-8.31</h1>
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<h1>Fluorescence Instruments Operation </h1>
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<p>1.We cleaved the Bm2, Bm6, and Bm7 plasmids to identify them.</p><p>
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<p>Fluorescence microscope and Thermo varioskan flash were operated by Han Kang in the Center of Growth, Metabolism and Aging (CGMA), Sichuan University.<p></div>
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2.We did the liquid culture for the bacteria containing Am4-Am11 plasmids.</p><p>
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3.We extracted the Am4-Am11 plasmids for storage.</p><p>
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4.We did the gel purification and linked the Bm2, Bm6, and Bm7 parts with pSB1C3.</p><p>
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5.We transformed our linkage products and extracted them.</p><p>
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6.We identified them by double cleavage and sent them to sequence.</p><p>
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Latest revision as of 19:30, 17 October 2014

The Notebook of

BioBrick Group

Finally we have to submit some biobricks that we made in our experiment. This notebook is focusing on constructing independent biobricks.There are about 30 biobricks that we plan to submit. In detail, we separate the into 4 groups:

Group one contains 8 different parts (Am4: Pcon+araC+pBAD; Am5: pRhl+RBS+mRFP+DT; Am6: pRHl/Las+RBS+GFP+DT; Am7: Pcon+RBS+LasR; Am8: RhlR+DT; Am9: RBS+amilCP+DT; Am10:RBS+amilCP+DT+Pcon+RBS; Am11: pRhl/Las+RBS);

Group two contains 8 different parts (Bm1: RBS+LasR+RhlR+DT; Bm2: Ptet+RBS+RhlR+DT; Bm3: Pcon+RBS_LasR+RhlR+DT; Bm4: pCin+CinR; Bm5: Pcon+RBS+Lac I; Bm6: pLac+RBS+luxI+DT; Bm7: RBS+CinI+DT; Bm8: pLux+RBS+LuxR+RBS+RhlI)

Week 1 8.21-8.24

1.We resuscitated the bacteria that contain Am4-Am11 parts.

2.We extracted all the plasmids and tested them by electrophoresis.

3.All the plasmids were cleaved by EcoR I and Pst I and we tested their qualities by electrophoresis.

4.We linked all the cleaved parts with pSB1C3 after we did the gel purification.

5.We transformed all the parts into E.coli. and extracted the plasmids which were tested by double cleavage.

6.We sent all the parts to sequence.

Week 2 8.25-8.31

1.We cleaved the Bm2, Bm6, and Bm7 plasmids to identify them.

2.We did the liquid culture for the bacteria containing Am4-Am11 plasmids.

3.We extracted the Am4-Am11 plasmids for storage.

4.We did the gel purification and linked the Bm2, Bm6, and Bm7 parts with pSB1C3.

5.We transformed our linkage products and extracted them.

6.We identified them by double cleavage and sent them to sequence.

Sichuan university

Retrieved from "http://2014.igem.org/Team:SCU-China/Biobricks"