Team:TU Darmstadt/Notebook/Methods/hydrochloric acid extraction

From 2014.igem.org

(Difference between revisions)
 
Line 14: Line 14:
<div id="wikicontent" class="grid_19">
<div id="wikicontent" class="grid_19">
-
<!--TYPO3SEARCH_begin--><div id="c246" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">37% hydrochloric acid extraction</h1></div><div class="csc-textpic-text"><div><p><b>Materials:&nbsp;</b></p></div><div><p><span style="font-size: 12px;">- 37% hydrochloric acid</span><br /><span style="font-size: 12px;">- Centrifuge</span><br /><span style="font-size: 12px;">- Tubes</span></p></div><div></div><div><p><b>Procedure:</b></p></div><div><p>- Spin down the bacterial solution at 4500g for 10 minutes.</p></div><div><p>- Remove culture media, weigh the remaining pellet and acetify with the same amount of 37% hydrochloric acid.&nbsp;</p></div><div><p>- Spin down the resolved pellet at 13,3g for 10 minutes and Remove supernatant.</p></div><div><p>The anthocyanidin is present in the organic and the aqueous phase; the color can be changed by adjusting the pH-value.&nbsp;</p></div><div></div></div></div><!--TYPO3SEARCH_end-->
+
<!--TYPO3SEARCH_begin--><div id="c246" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">37% Hydrochloric Acid Extraction</h1></div><div class="csc-textpic-text"><div><p><b>Materials:&nbsp;</b></p></div><div><p><span style="font-size: 14px;">- 37% hydrochloric acid</span><br /><span style="font-size: 14px;">- Centrifuge</span><br /><span style="font-size: 14px;">- Tubes</span></p></div><div></div><div><p><b>Procedure:</b></p></div><div><p>- Spin down the bacterial solution at 4500g for 10 minutes.</p></div><div><p>- Remove culture media, weigh the remaining pellet and acetify with the same amount of 37% hydrochloric acid.&nbsp;</p></div><div><p>- Spin down the resolved pellet at 13,3g for 10 minutes and Remove supernatant.</p></div><div><p>The anthocyanidin is present in the organic and the aqueous phase; the color can be changed by adjusting the pH-value.&nbsp;</p></div><div></div></div></div><!--TYPO3SEARCH_end-->
</div>
</div>
</html>
</html>

Latest revision as of 00:49, 18 October 2014

Home


37% Hydrochloric Acid Extraction

Materials: 

- 37% hydrochloric acid
- Centrifuge
- Tubes

Procedure:

- Spin down the bacterial solution at 4500g for 10 minutes.

- Remove culture media, weigh the remaining pellet and acetify with the same amount of 37% hydrochloric acid. 

- Spin down the resolved pellet at 13,3g for 10 minutes and Remove supernatant.

The anthocyanidin is present in the organic and the aqueous phase; the color can be changed by adjusting the pH-value.