Team:Hong Kong HKUST/riboregulator/parts

From 2014.igem.org

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<div class = "catalog_table_area">
<div class = "catalog_table_area">
-
<div class='content_2'><h3><font color="white">Parts that we designed and submitted</font></h3></div>
 
<table class= "catalog_table">
<table class= "catalog_table">
<thead>
<thead>
Line 34: Line 33:
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379015">BBa_K1379015</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379015">BBa_K1379015</a></td>  
-
  <td>Lock 3C RBS</td>       
+
  <td>RBS</td>       
-
  <td>B0032 derivate for Lock 3C</td>    
+
  <td>RBS(Lock 3c)</td>    
  <td>12</td>
  <td>12</td>
</tr>
</tr>
Line 41: Line 40:
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379018">BBa_K1379018</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379018">BBa_K1379018</a></td>  
-
  <td>HKUST Lock 1</td>       
+
  <td>RNA</td>       
-
  <td>Cis-Repressor HKUST Lock 1</td>    
+
  <td>HKUST Lock 1</td>    
  <td>45</td>
  <td>45</td>
</tr>  
</tr>  
Line 48: Line 47:
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379019">BBa_K1379019</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379019">BBa_K1379019</a></td>  
-
  <td>HKUST Key 1</td>       
+
  <td>RNA</td>       
-
  <td>Trans-Activator for HKUST Key 1</td>    
+
  <td>HKUST Key 1</td>    
  <td>82</td>
  <td>82</td>
</tr>  
</tr>  
Line 55: Line 54:
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379020">BBa_K1379020</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379020">BBa_K1379020</a></td>  
-
  <td>Cis-repressed GFP Lock 1</td>       
+
  <td>Intermediate</td>       
-
  <td>Cis-repressed GFP Generator Lock 1</td>    
+
  <td>Lock 1-GFP-B0015</td>    
  <td>903</td>
  <td>903</td>
</tr>
</tr>
Line 62: Line 61:
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379022">BBa_K1379022</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379022">BBa_K1379022</a></td>  
-
  <td>Cis-repressed GFP Generator Lock 3</td>       
+
  <td>Intermediate</td>       
-
  <td>Cis-repressed GFP Generator Lock 3 </td>    
+
  <td>Lock 3-GFP-B0015</td>    
-
  <td>905</td>
+
  <td>903</td>
</tr>
</tr>
 +
<tr>
<tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379023">BBa_K1379023</a></td>
 +
  <td>Intermediate</td>     
 +
  <td>Key 3-B0015</td>  
 +
  <td>231</td>
 +
</tr>
 +
 +
                                <tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379024">BBa_K1379024</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379024">BBa_K1379024</a></td>  
-
  <td>Cis-repressed GFP Lock 3C</td>       
+
  <td>Intermediate</td>       
-
  <td>Cis-repressed GFP Generator Lock 3C</td>    
+
  <td>Lock 3c-GFP-B0015</td>    
  <td>905</td>
  <td>905</td>
</tr>
</tr>
 +
 +
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379026">BBa_K1379026</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379026">BBa_K1379026</a></td>  
-
  <td>Cis-repressed GFP Lock MCR</td>       
+
  <td>Intermediate</td>       
-
  <td>Cis-repressed GFP Generator MCR</td>    
+
  <td>K175031-GFP-B0015</td>    
  <td>910</td>
  <td>910</td>
</tr>  
</tr>  
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379028">BBa_K1379028</a></td>
 
-
  <td>Cis-repressed GFP HKUST Lock 1</td>     
 
-
  <td>Cis-repressed GFP Generator HKUST Lock 1</td>  
 
-
  <td>908</td>
 
-
</tr>
 
-
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379046">BBa_K1379046</a></td>
 
-
  <td>Lock 1 RBS GFP generator</td>     
 
-
  <td>GFP generator using a Lock 1 modified RBS</td>  
 
-
  <td>873</td>
 
-
</tr>
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379047">BBa_K1379047</a></td>
 
-
  <td>Lock 3C RBS GFP generator</td>     
 
-
  <td>GFP generator using a Lock 3C modified RBS</td>  
 
-
  <td>875</td>
 
-
</tr>
 
-
</table>
+
                                <tr>
-
 
+
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379027">BBa_K1379027</a></td>  
-
<br><br>
+
  <td>Intermediate</td>       
-
<div class='content_2'><h3><font color="white">Parts that we constructed and submitted</font></h3></div>
+
  <td>K175032-B0015</td>    
-
<table class= "catalog_table">
+
  <td>221</td>
-
<thead>
+
-
<tr>
+
-
<th scope="col">Name</th>
+
-
<th scope="col">Type</th>
+
-
<th scope="col">Description</th>
+
-
<th scope="col">Length</th>
+
-
</tr>
+
-
</thead>
+
-
<tbody>
+
-
 
+
-
<tr>
+
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_I0500">BBa_I0500</a></td>  
+
-
  <td>Inducible pBad/araC promoter</td>       
+
-
  <td>Inducible pBad/araC promoter</td>  
+
-
  <td>1210</td>
+
-
</tr>
+
-
+
-
<tr>
+
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01010">BBa_J01010</a></td>
+
-
  <td>Riboregulator Lock 1</td>     
+
-
  <td>Biobrick version of Isaacs' riboregulator cis repressed lock, crR12.</td>  
+
-
  <td>40</td>
+
-
</tr>
+
-
+
-
<tr>
+
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01080">BBa_J01080</a></td>
+
-
  <td>Lock3</td>     
+
-
  <td>Biobrick version of Isaacs' riboregulator cis repressed lock, crR12, and modified version of Riboregulator Lock 1.</td>    
+
-
  <td>40</td>
+
</tr>
</tr>
<tr>
<tr>
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01086">BBa_J01086</a></td>  
+
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379028">BBa_K1379028</a></td>  
-
  <td>Key3</td>       
+
  <td>Intermediate</td>       
-
  <td>Biobricked version of Isaacs' riboregulator trans activating key, taR12, and modified version of Riboregulator Key 1.</td>  
+
  <td>HKUST Lock 1-GFP-B0015</td>    
-
  <td>94</td>
+
  <td>908</td>
-
</tr>
+
-
 
+
-
<tr>
+
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K175031">BBa_K175031</a></td>
+
-
  <td>Lock for medium RBS (B0032) from the lock/key library TUD09</td>     
+
-
  <td>This biobrick generates a mRNA which forms a secondary structure blocking the (medium) RBS.</td>  
+
-
  <td>47</td>
+
-
</tr>
+
-
+
-
<tr>
+
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K175032">BBa_K175032</a></td>
+
-
  <td>Key for lock of medium (K175031) RBS (B0032) from the lock/key library TUD09</td>     
+
-
  <td>This biobrick generates a mRNA which forms a secondary structure that opens LMR (K175031).</td>  
+
-
  <td>84</td>
+
-
</tr>
+
-
+
-
<tr>
+
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J23080">BBa_J23080</a></td>
+
-
  <td>B0034 derived RBS to match spacing of J01122</td>     
+
-
  <td>RBS spacing modification to [RBS] such that the spacing between the shine-delgarno and the ORF is the same as it is in J01122.</td>  
+
-
  <td>10</td>
+
-
</tr>
+
-
+
-
<tr>
+
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379048">BBa_K1379048</a></td>
+
-
  <td>Cis repressed GFP, with Trans-activator</td>     
+
-
  <td>Repression measurement device 1 with trans activator</td>    
+
-
  <td>2403</td>
+
</tr>  
</tr>  
-
</tbody>
 
-
</table>
 
-
 
-
<br><br>
 
-
<div class='content_2'><h3><font color="white">Parts that already exist in Parts Registry and are currently under characterization</font></h3></div>
 
-
<table class= "catalog_table">
 
-
<thead>
 
-
<tr>
 
-
<th scope="col">Name</th>
 
-
<th scope="col">Type</th>
 
-
<th scope="col">Description</th>
 
-
<th scope="col">Length</th>
 
-
</tr>
 
-
</thead>
 
-
<tbody>
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01008">BBa_J01008</a></td>
 
-
  <td>Riboregulator Lock 1</td>     
 
-
  <td>Lock 1</td>  
 
-
  <td>94</td>
 
-
</tr>
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J23031">BBa_J23031</a></td>
 
-
  <td>Lock 3c</td>     
 
-
  <td>lock3c</td>  
 
-
  <td>42</td>
 
-
</tr>
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J23008">BBa_J23008</a></td>
 
-
  <td>Key 3c</td>     
 
-
  <td>Encodes a short RNA sequence that "unlocks" the biobrick part J01122 (lock3) which is locked RFP.</td>  
 
-
  <td>42</td>
 
-
</tr>
 
-
 
-
</tbody>
 
-
</table>
 
-
 
-
<div class = "catalog_table_area">
 
-
<div class='content_2'><h3><font color="white">Parts that we designed but did not submit</font></h3></div>
 
-
<table class= "catalog_table">
 
-
<thead>
 
-
<tr>
 
-
<th scope="col">Name</th>
 
-
<th scope="col">Type</th>
 
-
<th scope="col">Description</th>
 
-
<th scope="col">Length</th>
 
-
</tr>
 
-
</thead>
 
-
<tbody>
 
-
 
-
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379021">BBa_K1379021</a></td>
 
-
  <td>Key 1 Double Terminator</td>     
 
-
  <td>Trans-Activator with terminator for lock 1</td>  
 
-
  <td>231</td>
 
-
</tr>
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379023">BBa_K1379023</a></td>
 
-
  <td>Key 3 double terminator</td>     
 
-
  <td>Trans-Activator with terminator for lock 3</td>  
 
-
  <td>231</td>
 
-
</tr>
 
-
 
-
<tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379025">BBa_K1379025</a></td>
 
-
  <td>Key 3c double terminator</td>     
 
-
  <td>Trans-Activator with terminator for lock 3C</td>  
 
-
  <td>231</td>
 
-
</tr>
 
-
 
-
                                <tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379027">BBa_K1379027</a></td>
 
-
  <td>MTA double terminator</td>     
 
-
  <td>Trans-Activator with terminator for MCR</td>  
 
-
  <td></td>
 
-
</tr>
 
-
 
                                 <tr>
                                 <tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379029">BBa_K1379029</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379029">BBa_K1379029</a></td>  
-
  <td>HKUST Key 1 double terminator</td>       
+
  <td>Intermediate</td>       
-
  <td>Trans-Activator with terminator for HKUST Lock 1</td>    
+
  <td>HKUST Key 1-B0015</td>    
-
  <td></td>
+
  <td>219</td>
</tr>
</tr>
                                 <tr>
                                 <tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379030">BBa_K1379030</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379030">BBa_K1379030</a></td>  
-
  <td>Cis-Repressing device 1</td>       
+
  <td>Measurement</td>       
-
  <td>Constitutive promoted, Cis-repressed GFP Coding device Lock 1</td>    
+
  <td>J23102-Lock 1-GFP-B0015</td>    
-
  <td></td>
+
  <td>946</td>
</tr>
</tr>
-
                                <tr>
+
                             
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379031">BBa_K1379031</a></td>
+
-
  <td>AraC Key 1</td>     
+
-
  <td>AraC inducible Trans-Activator device, Key 1</td>  
+
-
  <td></td>
+
-
</tr>
+
                                 <tr>
                                 <tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379032">BBa_K1379032</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379032">BBa_K1379032</a></td>  
-
  <td>Cis-Repressing device 3</td>       
+
  <td>Measurement</td>       
-
  <td>Constitutive promoted, Cis-repressed GFP Coding device Lock 3</td>    
+
  <td>J23102-Lock 3-GFP-B0015</td>    
-
  <td></td>
+
  <td>948</td>
</tr>
</tr>
                                 <tr>
                                 <tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379033">BBa_K1379033</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379033">BBa_K1379033</a></td>  
-
  <td>AraC Key 3</td>       
+
  <td>Intermediate</td>       
-
  <td>AraC inducible Trans-Activator device, Key 3</td>    
+
  <td>pBad/araC-Key 3</td>    
-
  <td></td>
+
  <td>1312</td>
</tr>
</tr>
                                 <tr>
                                 <tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379034">BBa_K1379034</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379034">BBa_K1379034</a></td>  
-
  <td>Cis-Repressing device 3C</td>       
+
  <td>Measurement</td>       
-
  <td>Constitutive promoted, Cis-repressed GFP Coding device Lock 3C</td>    
+
  <td>J23102-Lock 3c-GFP-B0015</td>    
-
  <td></td>
+
  <td>948</td>
</tr>
</tr>
                                 <tr>
                                 <tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379035">BBa_K1379035</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379035">BBa_K1379035</a></td>  
-
  <td>AraC Key 3C</td>       
+
  <td>Intermediate</td>       
-
  <td>AraC inducible Trans-Activator device for, Key 3C</td>    
+
  <td>pBad/araC-Key 3c</td>    
-
  <td></td>
+
  <td>1312</td>
</tr>
</tr>
                                 <tr>
                                 <tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379036">BBa_K1379036</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379036">BBa_K1379036</a></td>  
-
  <td>Cis-Repressing device MCR</td>       
+
  <td>Measurement</td>       
-
  <td>Constitutive promoted, Cis-represed GFP Coding device Lock MCR</td>    
+
  <td>J23102-K175031-GFP-B0015</td>    
-
  <td></td>
+
  <td>953</td>
</tr>
</tr>
                                 <tr>
                                 <tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379037">BBa_K1379037</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379037">BBa_K1379037</a></td>  
-
  <td>AraC MTA</td>       
+
  <td>Intermediate</td>       
-
  <td>AraC inducible Trans-Activator device, MTA</td>    
+
  <td>pBad/araC-K175032</td>    
-
  <td></td>
+
  <td>1302</td>
</tr>
</tr>
                                 <tr>
                                 <tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379038">BBa_K1379038</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379038">BBa_K1379038</a></td>  
-
  <td>Cis-Repressing device HKUST 1</td>       
+
  <td>Measurement</td>       
-
  <td>Constitutive promoted, Cis-repressed GFP Coding device HKUST Lock 1</td>    
+
  <td>J23102-HKUST Lock 1-GFP-B0015</td>    
-
  <td></td>
+
  <td>951</td>
</tr>
</tr>
                                 <tr>
                                 <tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379039">BBa_K1379039</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379039">BBa_K1379039</a></td>  
-
  <td>AraC HKUST Key 1</td>       
+
  <td>Intermediate</td>       
-
  <td>AraC inducible Trans-Activator device, HKUST Key 1</td>    
+
  <td>pBad/araC-HKUST Key 1</td>    
-
  <td></td>
+
  <td>1300</td>
-
</tr>
+
</tr>                          
-
                                <tr>
+
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379040">BBa_K1379040</a></td>  
+
<tr>
-
  <td>Repression device 1</td>       
+
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379046">BBa_K1379046</a></td>  
-
  <td>Repression measurement device 1</td>    
+
  <td>Intermediate</td>       
-
  <td></td>
+
  <td>RBS(Lock 1)-GFP-B0015</td>  
-
</tr>
+
  <td>873</td>
 +
</tr>
 +
 +
<tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379047">BBa_K1379047</a></td>
 +
  <td>Intermediate</td>     
 +
  <td>RBS(Lock 3c)-GFP-B0015</td>    
 +
  <td>875</td>
 +
</tr>  
-
                                <tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379041">BBa_K1379041</a></td>
 
-
  <td>Repression device 3</td>     
 
-
  <td>Repression measurement device 3</td>  
 
-
  <td></td>
 
-
</tr>
 
-
                                <tr>
+
<tr>
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379042">BBa_K1379042</a></td>  
+
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379048">BBa_K1379048</a></td>  
-
  <td>Repression device 3C</td>       
+
  <td>Measurement</td>       
-
  <td>Repression measurement device 3C</td>    
+
  <td>J23102-Lock 1-GFP-B0015-pBad/araC-Key 1-B0015</td>    
-
  <td></td>
+
  <td>2403</td>
</tr>
</tr>
-
                                <tr>
+
</table>
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379043">BBa_K1379043</a></td>
+
-
  <td>Repression device Medium</td>     
+
-
  <td>Repression measurement device Medium</td>  
+
-
  <td></td>
+
-
</tr>
+
-
                                <tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379044">BBa_K1379044</a></td>
 
-
  <td>Cis-Repression device HKUST 1</td>     
 
-
  <td>Repression measurement device HKUST 1</td>  
 
-
  <td></td>
 
-
</tr>
 
-
 
-
                                <tr>
 
-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379049">BBa_K1379049</a></td>
 
-
  <td>Cis repressed GFP lock 1, with Trans-activator key 1</td>     
 
-
  <td>Cis repressed GFP lock 1, with Trans-activator key 1</td>  
 
-
  <td></td>
 
-
</tr>
 
-
 
-
</table>
 
</div>
</div>

Latest revision as of 22:06, 17 October 2014



Riboregulator Parts

Name Type Description Length
BBa_K1379015 RBS RBS(Lock 3c) 12
BBa_K1379018 RNA HKUST Lock 1 45
BBa_K1379019 RNA HKUST Key 1 82
BBa_K1379020 Intermediate Lock 1-GFP-B0015 903
BBa_K1379022 Intermediate Lock 3-GFP-B0015 903
BBa_K1379023 Intermediate Key 3-B0015 231
BBa_K1379024 Intermediate Lock 3c-GFP-B0015 905
BBa_K1379026 Intermediate K175031-GFP-B0015 910
BBa_K1379027 Intermediate K175032-B0015 221
BBa_K1379028 Intermediate HKUST Lock 1-GFP-B0015 908
BBa_K1379029 Intermediate HKUST Key 1-B0015 219
BBa_K1379030 Measurement J23102-Lock 1-GFP-B0015 946
BBa_K1379032 Measurement J23102-Lock 3-GFP-B0015 948
BBa_K1379033 Intermediate pBad/araC-Key 3 1312
BBa_K1379034 Measurement J23102-Lock 3c-GFP-B0015 948
BBa_K1379035 Intermediate pBad/araC-Key 3c 1312
BBa_K1379036 Measurement J23102-K175031-GFP-B0015 953
BBa_K1379037 Intermediate pBad/araC-K175032 1302
BBa_K1379038 Measurement J23102-HKUST Lock 1-GFP-B0015 951
BBa_K1379039 Intermediate pBad/araC-HKUST Key 1 1300
BBa_K1379046 Intermediate RBS(Lock 1)-GFP-B0015 873
BBa_K1379047 Intermediate RBS(Lock 3c)-GFP-B0015 875
BBa_K1379048 Measurement J23102-Lock 1-GFP-B0015-pBad/araC-Key 1-B0015 2403

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