Team:Caltech/Project/Methods and Methods
From 2014.igem.org
(Difference between revisions)
m |
|||
(6 intermediate revisions not shown) | |||
Line 43: | Line 43: | ||
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='black'; modeling.style.color='white'" onMouseOut="this.bgColor='white'; modeling.style.color='black'" bgColor=white> | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='black'; modeling.style.color='white'" onMouseOut="this.bgColor='white'; modeling.style.color='black'" bgColor=white> | ||
- | <a id='modeling' href="https://2014.igem.org/Team:Caltech/ | + | <a id='modeling' href="https://2014.igem.org/Team:Caltech/TXTL"style="color:#000000"> TXTL Promoter Characterization</a></td> |
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='black'; notebook.style.color='white'" onMouseOut="this.bgColor='white'; notebook.style.color='black'" bgColor=white> | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='black'; notebook.style.color='white'" onMouseOut="this.bgColor='white'; notebook.style.color='black'" bgColor=white> | ||
Line 71: | Line 71: | ||
<tr> | <tr> | ||
<td width="20%" valign="top"> | <td width="20%" valign="top"> | ||
- | <a href = "https://2014.igem.org/Team:Caltech/Project"> | + | <a href = "https://2014.igem.org/Team:Caltech/Project">Project Overview</a> |
<br><br> | <br><br> | ||
<a href = "https://2014.igem.org/Team:Caltech/Project/Details">Project Details</a> | <a href = "https://2014.igem.org/Team:Caltech/Project/Details">Project Details</a> | ||
Line 79: | Line 79: | ||
<a href = "https://2014.igem.org/Team:Caltech/Project/Experiments">The Experiments</a> | <a href = "https://2014.igem.org/Team:Caltech/Project/Experiments">The Experiments</a> | ||
<br><br> | <br><br> | ||
- | <a href = "https://2014.igem.org/Team:Caltech/Project/Results">Results | + | <a href = "https://2014.igem.org/Team:Caltech/Project/Results">Our Results</a> |
- | + | ||
- | + | ||
<br><br> | <br><br> | ||
<a href = "https://2014.igem.org/Team:Caltech/Project/Conclusions">Conclusions</a> | <a href = "https://2014.igem.org/Team:Caltech/Project/Conclusions">Conclusions</a> | ||
Line 205: | Line 203: | ||
<ul><li> Add 2 μL of plasmid to 20 μL of cells</li> | <ul><li> Add 2 μL of plasmid to 20 μL of cells</li> | ||
<li> Stir with pipette tip</li> | <li> Stir with pipette tip</li> | ||
- | <li> Keep cells on ice</li> | + | <li> Keep cells on ice for 5-10 minutes</li> |
+ | <li> Add 4 volumes of SOC medium and incubate in shaker at 37°C for 1 hour. This step is optional if plating onto CARB. </li> | ||
<li> Plate with glass beads </li> | <li> Plate with glass beads </li> | ||
</ul> | </ul> | ||
Line 214: | Line 213: | ||
<li> Add 100 μL of SOC medium, then incubate in a 37°C shaker for 30-90 minutes to recover </li> | <li> Add 100 μL of SOC medium, then incubate in a 37°C shaker for 30-90 minutes to recover </li> | ||
<li> Plate with glass beads </li> | <li> Plate with glass beads </li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr><td bgColor="#777777" colspan="2" height="1px"></td></tr> | ||
+ | |||
+ | <tr> | ||
+ | <td width="25%" valign="top"> | ||
+ | <h4>Liquid Culture Inoculation</h4> | ||
+ | </td> | ||
+ | <td valign="top"> | ||
+ | <ul><li> Add 5 μL antibiotic to 5 mL LB media </li> | ||
+ | <li> Pick colony from solid culture with pipette tip and mix into liquid </li> | ||
+ | <li> Incubate in 37°C shaker overnight</li> | ||
</ul> | </ul> | ||
</td> | </td> |
Latest revision as of 21:43, 17 October 2014
![]() | |||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||
Project Overview
Project Details Materials and Methods The Experiments Our Results Conclusions References |
|