Team:Jilin China/DESIGN

From 2014.igem.org

(Difference between revisions)
(Created page with "{{CSS/Main}} <html> <head> <style type="text/css"> .observation{} .observe1{width:600px;margin:0 auto} #coop{font-size:18px;padding:0px 20px;} .clear{clear:both} </style> <style...")
 
(25 intermediate revisions not shown)
Line 118: Line 118:
<div class="menu">
<div class="menu">
-
<!---吉大logo---->
+
<!---比赛logo---->
<div class="logo">
<div class="logo">
-
<img style="height:80px;"src="https://static.igem.org/mediawiki/2014/a/a3/Uea_igem-logo.png">
+
<a href="https://2014.igem.org/Main_Page"><img style="height:80px;"src="https://static.igem.org/mediawiki/2014/a/a3/Uea_igem-logo.png"></a>
</div>
</div>
-
<!---比赛logo---->
+
<!---吉大logo---->
<div style="float:left;">
<div style="float:left;">
-
<img style="height:80px;"src="https://static.igem.org/mediawiki/2014/6/60/Jilin_China_igem_logo.gif">
+
<a href="https://2014.igem.org/Team:Jilin_China"><img style="height:80px;"src="https://static.igem.org/mediawiki/2014/6/60/Jilin_China_igem_logo.gif"></a>
</div>
</div>
<table id="menu" cellspacing="0" height="135px">
<table id="menu" cellspacing="0" height="135px">
Line 138: Line 138:
<li><a style="margin-top:20px;" class="hide" >PROJECT<span class="caret"></span></a>  
<li><a style="margin-top:20px;" class="hide" >PROJECT<span class="caret"></span></a>  
<ul>  
<ul>  
-
<li><a style="margin-top:20px;" href="https://2014.igem.org/Team:Jilin_China/NOTEBOOK" title="a coded list of spies">NOTEBOOK</a></li>  
+
<li><a style="margin-top:20px;" href="https://2014.igem.org/Team:Jilin_China/Outline" title="a coded list of spies">OUTLINE</a></li>  
-
<li><a href="https://2014.igem.org/Team:Jilin_China/RESULT" title="a horizontal vertical menu">RESULT</a></li>  
+
<li><a href="https://2014.igem.org/Team:Jilin_China/RESULT" title="a horizontal vertical menu">CONTRUST</a></li>
 +
<li><a href="https://2014.igem.org/Team:Jilin_China/NOTEBOOK" title="a horizontal vertical menu">TEST</a></li>
 +
<li><a href="https://2014.igem.org/Team:Jilin_China/Blueprint" title="a horizontal vertical menu">BLUEPRINT</a></li>  
</ul>  
</ul>  
</li>  
</li>  
Line 176: Line 178:
<!-- You can replace this image with your team's logo! -->
<!-- You can replace this image with your team's logo! -->
-
<h3>Welcome!  <br>Team Jilin China</h3>
+
<h3>Welcome!  <br>Team Jilin_China</h3>
Line 188: Line 190:
<!--  Block of content 1 -->
<!--  Block of content 1 -->
<table width="100%"  cellspacing="0" height="0px">
<table width="100%"  cellspacing="0" height="0px">
-
<tr><td bgColor="#FEE5AD"></td>
+
<tr><td></td>
<td width="975px" align="left" bgColor="#FEE5AD" >
<td width="975px" align="left" bgColor="#FEE5AD" >
<table  width="975px"  cellspacing="0" height="0px">
<table  width="975px"  cellspacing="0" height="0px">
Line 206: Line 208:
<!-- Content goes here -->
<!-- Content goes here -->
<div id="coop">
<div id="coop">
-
 
+
<center><h3>How to find and degradation of microcystins LR?</h3></center>
-
        <div class="observation"><center><h3>IGEM Background</h3></center> </div>
+
<p><img style="height:117px;" src="https://static.igem.org/mediawiki/2014/1/14/Design1.jpg"><img height="116" src="https://static.igem.org/mediawiki/2014/4/44/Design3.jpg"><br>
-
<div class="observe1"><img src="https://static.igem.org/mediawiki/2014/b/b4/Simonsong-idea-2.jpg"/></div>
+
   Microcystin-LR (MC-LR) is a naturally occurring  toxin released after the algal cells break down, which can do great damage to liver,very stable and in some extent is very resistant to high temperature and pH change, so it brings the water purification into a very embarrassing situation. <br>
-
 
+
  Mlr promoter is able to detect whether  water containing microcystin LR and start to expressthe GFP-mlrA fusion protein. GFP can emit green fluorescence, very simple and very sensitive to tell us the  water contains toxic microcystin LR. MlrA can directly degrade microcystin  LRand eliminate its toxicity, eliminate the toxins in water pollution.<br>
-
 
+
   The mlr promoter-GFP-mlrA was be cloned  into the <em>E. coli-L. lactis</em> shuttle  expression vector pMG36e, so it can be able to express in E. coli and <em>Lactococcus lactis</em>, which Can easily be  constructed in the laboratory, at the same time to avoid secondary pollution in  the natural environment of <em>E. coli</em>.<br>
-
<div class="clear"> <p>In the past few years, algal bloom caused by eutrophication has widely spread across the world and the situation became even worse after climate warming. It is reported that the algal bloom is taking place almost every main water globally, including Lake Victoria in Africa, Baltic, Lake Erie, Great Lakes and Ouji seeking Avon in Florida. The problem is that the algal can release a series of poisonous toxin called cyanotoxin, leading to serious diseases although they are not parasitic as other pathogenic bacteria. </p>  
+
  <center><img width="500" height="500" src="https://static.igem.org/mediawiki/2014/9/9d/Picture6.png" align="left" hspace="12"></center>
-
   <p>And the microcystin is the most common one in these toxin which is very toxic and predominant. MC is a kind of intracellular toxin, released after the algal cells break down. It can do great damage to liver in the way of teratogenic, carcinogenic, mutagenic. In addition, MC is very stable and in some extent is very resistant to high temperature and pH change, so it brings the water purification into a very embarrassing situation. In fact, some residues of MC can still be detected after processing of water plant chemically and physically. There is no doubt that an efficient method to deal with water polluted by MC is in urgent need. And that is exactly what our group would try to devote ourselves in. </p>  
+
  <a name="_GoBack"></a><br clear="all">
-
   <p>After several days’discussion and skipping of many references,we were glad to find that in nature there are several germs can degrade MC efficiently, such as Sphingomonas, P seudomonas and Burkholderia. Further exploring these germs, we found that their ability to degrade MC is originated from several hydrolases(MlrA、MlrB and MlrC). These enzymes inspirited us a lot and finally our group decided to construct a genetically engineered bacteria based on these special enzymes,which can express these hydrolases efficiently to solve this kind of water pollution. And our work begins! </p></div>
+
</p>
-
 
+
-
<div class="observe1"><img src="https://static.igem.org/mediawiki/2014/e/eb/Simonsong-idea-1.png"/></div>
+
-
 
+
-
 
+
-
<div id="clear"><p>References: </p>
+
-
<p>[1]M icrobial degradation of m icrocystins in water environm ent: A review. KONG Yun, XU Xiang-yang, ZHU L iang, XU Jing, LIN H a-i zhuan (Departm ent of Environmental Engineering,Zhejiang University, H angzhou 310058, China ). -Chin. J. App l. E col. , 2011, 22( 6) : 1646- 1652 </p>
+
-
<p>[2]Effects of Cyanobacteria Pollution and ControlMethods：ZHOU Guo-ding(Changzhou E cotechnology App lication R esearch Instn teti, Changzhou, J iang su 213022, China ) </p>
+
-
<p>[3]Advances in Pollution of Cyanobacterial Blooms-Producing Microcystins and their Ecotoxicological Effects on Aquatic Organisms：Jiang Jinlin1 Song Rui1 Ren Jinghua2 Wang Xiaorong1Yang Liuyan1 (1. State Key Laboratory of Pollution Control and Resources Reuse，School of Environment，Nanjng University，Nanjing 210093，China; 2. College of Resource and Environment，Shaanxi University of Science and Technology，Xi′an 712081，China) </p>
+
-
<p>[4] Ho L, Meyn T, Keegan A,etal. Bacterial degradation of microcystin toxins within a biologically active sand filter. Water Research, 2006, 40: 768- 774 </p>
+
-
<p>[ 5] Bourne DG, B lakeley RL, Riddles P, etal. Biodegradation of the cyanobacterial toxin microcystin LR innatural water and biologically active slow sand filters. Water Research, 2006, 40: 1294- 1302 </p>
+
-
<p>[6] ChenW, Song LR, Peng L, etal. Reduction in microcystin concentrations in large and shallow lakes: Water and sediment interface contributions. Water Research,
+
-
2008, 42: 763- 773 </p></div>
+
-
 
+
-
 
+
-
 
+
-
</div>
+
-
 
+
-
 
+
</td>   
</td>   
-
 
-
 
-
 
</table>
</table>
-
 
-
 
</td>
</td>
-
<td bgColor="#FEE5AD"></td>
+
<td ></td>
</tr>
</tr>
</table>
</table>
 +
</div>
<!-- end of Block-->
<!-- end of Block-->
</html>
</html>

Latest revision as of 03:35, 18 October 2014

Welcome!
Team Jilin_China

How to find and degradation of microcystins LR?


Microcystin-LR (MC-LR) is a naturally occurring toxin released after the algal cells break down, which can do great damage to liver,very stable and in some extent is very resistant to high temperature and pH change, so it brings the water purification into a very embarrassing situation.
Mlr promoter is able to detect whether water containing microcystin LR and start to expressthe GFP-mlrA fusion protein. GFP can emit green fluorescence, very simple and very sensitive to tell us the water contains toxic microcystin LR. MlrA can directly degrade microcystin LRand eliminate its toxicity, eliminate the toxins in water pollution.
The mlr promoter-GFP-mlrA was be cloned into the E. coli-L. lactis shuttle expression vector pMG36e, so it can be able to express in E. coli and Lactococcus lactis, which Can easily be constructed in the laboratory, at the same time to avoid secondary pollution in the natural environment of E. coli.


Top

Retrieved from "http://2014.igem.org/Team:Jilin_China/DESIGN"