Team:LIKA-CESAR-Brasil/TheRobot

From 2014.igem.org

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Line 12: Line 12:
<link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.2.0/css/bootstrap.min.css">
<link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.2.0/css/bootstrap.min.css">
-
    <link href="https://dl.dropbox.com/s/3g6ibzuen1skq16/lika.css" rel="stylesheet">
+
<style type="text/css">
 +
body {
 +
font-size:12px;
 +
color:#111;
 +
height:100%;
 +
background:#ededed;
 +
/*min-width: 1326px;*/
 +
}
 +
.container {
 +
width:80%;
 +
background: rgb(255,255,255); /* Old browsers */
 +
background: -moz-linear-gradient(top,  rgba(255,255,255,1) 0%, rgba(237,237,237,1) 100%); /* FF3.6+ */
 +
background: -webkit-gradient(linear, left top, left bottom, color-stop(0%,rgba(255,255,255,1)), color-stop(100%,rgba(237,237,237,1))); /* Chrome,Safari4+ */
 +
background: -webkit-linear-gradient(top,  rgba(255,255,255,1) 0%,rgba(237,237,237,1) 100%); /* Chrome10+,Safari5.1+ */
 +
background: -o-linear-gradient(top,  rgba(255,255,255,1) 0%,rgba(237,237,237,1) 100%); /* Opera 11.10+ */
 +
background: -ms-linear-gradient(top,  rgba(255,255,255,1) 0%,rgba(237,237,237,1) 100%); /* IE10+ */
 +
background: linear-gradient(to bottom,  rgba(255,255,255,1) 0%,rgba(237,237,237,1) 100%); /* W3C */
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filter: progid:DXImageTransform.Microsoft.gradient( startColorstr='#ffffff', endColorstr='#ededed',GradientType=0 ); /* IE6-9 */
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}
-
  </head>
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.navbar-brand {
 +
padding: 5px;
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}
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.col-md-10  {
 +
width:100%;
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padding: 20px 70px 20px 50px;
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min-height:200px;
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padding-bottom:50px;
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}
 +
.col-md-4 {
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padding-bottom:70px;
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}
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.navbar-inverse {
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    background-color: #ededed;
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border-color: #ededed;
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}
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.navbar-inverse .navbar-nav > .active > a, .navbar-inverse .navbar-nav > .active > a:hover, .navbar-inverse .navbar-nav > .active > a:focus {
 +
    color: #006600;
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    background-color: #8cff00;
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}
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.navbar-inverse .navbar-nav > .open > a, .navbar-inverse .navbar-nav > .open > a:hover, .navbar-inverse .navbar-nav > .open > a:focus {
 +
    color: #006600;
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    background-color: #8cff00;
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}
 +
.navbar-inverse .navbar-nav > li > a:hover, .navbar-inverse .navbar-nav > li > a:focus {
 +
    color: #006600;
 +
    background-color: transparent;
 +
}
 +
.navbar-inverse .navbar-nav > li > a:focus {
 +
    color: #006600;
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    background-color: transparent;
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}
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.nav > li > a {
 +
    display: block;
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    padding: 25px 15px;
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    position: relative;
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}
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.menu{
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color:black;
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z-index:999;
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position:relative;
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float:right;
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margin:auto;
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}
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  <body>
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.videohome{
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background:#ededed;
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}
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.menu ul{
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margin:0 auto;
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}
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.jumbotron {
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background:url("https://static.igem.org/mediawiki/2014/7/7a/Banner_top.jpg");
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border-top: solid 5px #8cff00;
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border-bottom: solid 5px #8cff00;
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background-size:cover;
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width:80%;
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height:331px;
 +
margin:auto;
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margin-top:40px;
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}
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.jumbotron02 {
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background:url("https://static.igem.org/mediawiki/2014/5/52/Banner_top02.jpg");
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border-top: solid 5px #8cff00;
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border-bottom: solid 5px #8cff00;
 +
background-size:cover;
 +
width:80%;
 +
height:331px;
 +
margin:auto;
 +
margin-top:80px;
 +
}
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.jumbotronSafety {
 +
background:url("https://static.igem.org/mediawiki/2014/7/7a/Banner_top.jpg");
 +
border-top: solid 5px #8cff00;
 +
border-bottom: solid 5px #8cff00;
 +
background-size:cover;
 +
width:80%;
 +
height:331px;
 +
margin:auto;
 +
margin-top:80px;
 +
}
 +
.jumbotronParts {
 +
background:url("https://static.igem.org/mediawiki/2014/b/be/Banner_top04.jpg");
 +
border-top: solid 5px #8cff00;
 +
border-bottom: solid 5px #8cff00;
 +
background-size:cover;
 +
width:80%;
 +
height:331px;
 +
margin:auto;
 +
margin-top:80px;
 +
}
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.jumbotronHuman {
 +
background:url("https://static.igem.org/mediawiki/2014/1/12/Banner_top05.jpg");
 +
border-top: solid 5px #8cff00;
 +
border-bottom: solid 5px #8cff00;
 +
background-size:cover;
 +
width:80%;
 +
height:331px;
 +
margin:auto;
 +
margin-top:80px;
 +
}
 +
.jumbotronProject {
 +
background:url("https://static.igem.org/mediawiki/2014/b/be/Banner_top04.jpg");
 +
border-top: solid 5px #8cff00;
 +
border-bottom: solid 5px #8cff00;
 +
background-size:cover;
 +
width:80%;
 +
height:331px;
 +
margin:auto;
 +
margin-top:80px;
 +
}
 +
.jumbotronAdvisors {
 +
background:url("https://static.igem.org/mediawiki/2014/3/3b/Banner_top03.jpg");
 +
border-top: solid 5px #8cff00;
 +
border-bottom: solid 5px #8cff00;
 +
background-size:cover;
 +
width:80%;
 +
height:331px;
 +
margin:auto;
 +
margin-top:80px;
 +
}
 +
.igem-logo{
 +
    background:url("https://static.igem.org/mediawiki/2014/4/4e/Banner_top_03.png") ;
 +
    background-color:transparent;
 +
    width: 50px;
 +
    height:50px;
 +
    z-index: 99;
 +
    background-repeat: no-repeat;
 +
}
 +
 +
/*
 +
.navbar .container {
 +
min-width: 1322px;
 +
    z-index: 9999;
 +
}
 +
*/
 +
 +
h2 {
 +
font-size: 26px;
 +
color:#339900;
 +
}
 +
.videocapa{
 +
width:35%;
 +
float:right;
 +
min-height:300px;
 +
margin-top:10px;
 +
}
 +
p {
 +
font-size: 12px;
 +
color:#444;
 +
}
 +
footer {
 +
background:#b8b8b8;
 +
width:100%;
 +
padding: 50px;
 +
  clear:both;
 +
position: relative;
 +
bottom:0;
 +
border-top: solid 8px #8cff00;
 +
}
 +
footer p {
 +
margin:auto;
 +
padding-top:10px;
 +
text-align:center;
 +
width:50%;
 +
color:#006600;
 +
}
 +
.firstHeading {
 +
display:none;
 +
}
 +
table{
 +
color:#888;
 +
font-size:16px;
 +
border: solid 1px #ccc;
 +
}
 +
h1 {
 +
font-size: 26px;
 +
color:#339900;
 +
}
 +
h2 {
 +
font-size: 22px;
 +
color:#339900;
 +
}
 +
h3 {
 +
font-size: 18px;
 +
color:#339900;
 +
}
 +
h4 {
 +
font-size: 16px;
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color:#339900;
 +
}
 +
 +
.listHorizontal{
 +
    list-style: none;
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    display:inline-block;
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    margin-left: 0.3em;
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}
 +
 +
.listHorizontal li{
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    padding: 0px 5px 0px 5px;
 +
}
 +
 +
div#globalWrapper {
 +
position: relative;
 +
font-size: 100%;
 +
width: 100%;
 +
margin: 0;
 +
padding: 0;
 +
padding-bottom: 10px;
 +
}
 +
 +
</style>
 +
  </head>
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  <body>
     <div class="navbar navbar-inverse navbar-fixed-top" role="navigation">
     <div class="navbar navbar-inverse navbar-fixed-top" role="navigation">
       <div class="container">
       <div class="container">
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         <li><a href="https://2014.igem.org/Team:LIKA-CESAR-Brasil/Safety">SAFETY</a></li>
         <li><a href="https://2014.igem.org/Team:LIKA-CESAR-Brasil/Safety">SAFETY</a></li>
         <li><a href="https://2014.igem.org/Team:LIKA-CESAR-Brasil/Atribuitons">ATRIBUITIONS</a></li>
         <li><a href="https://2014.igem.org/Team:LIKA-CESAR-Brasil/Atribuitons">ATRIBUITIONS</a></li>
 +
        <li class="igem-logo"><a href="https://2014.igem.org/"></a></li>
             </ul>
             </ul>
           </div><!--/.nav-collapse -->
           </div><!--/.nav-collapse -->

Latest revision as of 02:12, 18 October 2014

LIKA | CESAR

NOTEBOOK

Materials:

1.Control Unit

  • 1.Arduino UNO

2.Power Supply

  • 1.Computer Power Supply Unit (ATX)
  • 2.Custom designed plate | 2.1. 5V and 12V conectors

3.Electric Syringe

  • 1.Stteper motor
  • 2.Endless worm
  • 3.Custom designed parts
  • 4.Syringe
  • 5.IV extension tubes
  • 6.3-Way Stopcock

4.Robotic Arm

  • 1.Servo motors
  • 2.Custom designed parts

5.Centrifuge

  • 1.Brushless motor
  • 2.Esc for motor
  • 3.Centrifuge arm (custom designed)
  • 4.Motor support (custom designed)
  • 5.PCR tube support (custom designed)
  • 2.Esc for motor

1.Done manually:

  • 1.Collect a 2 mL blood sample from patient in EDTA tube with 2 mL of RNA later;
  • 2.Take a 500 μL fraction from the collected blood sample and transfer to 1.5mL PCR tubes;

2.Robot:

  • 1.Adds 1mL of lysis solution (custom designed) in the 500 μL of blood, mix and wait 10 minutes;
  • 2.After 10 minutes, heat the PCR tube at 56°C for 10 minutes;
  • 3.Centrifuge at 8.000 rpm for 5 minutes;
  • 4.Heat the PCR tube at 98ºC for 1min;
  • 1.Take 5 μL of the supernatant part of the liquid inside the PCR tube to the biosensor.
Marca LIKA Marca CESAR

Retrieved from "http://2014.igem.org/Team:LIKA-CESAR-Brasil/TheRobot"