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Team:Groningen/Template/MODULE/projects/toolbox/vector
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To get BioBricks in <i>L. lactis</i>, a compatible backbone is needed. For this, | To get BioBricks in <i>L. lactis</i>, a compatible backbone is needed. For this, | ||
| - | we used a well known plasmid that is known to work in <i>L. lactis</i>, pIL253, | + | we used a well known plasmid that is known to work in <i>L. lactis</i>, <a href="http://parts.igem.org/Part:BBa_K1365301">pIL253</a>, |
and made this BioBrick compatible. The plasmid is a shuttle vector that can be | and made this BioBrick compatible. The plasmid is a shuttle vector that can be | ||
used in <i>L. lactis</i> and <i>Bacillus subtilis</i>. It carries erythromycin | used in <i>L. lactis</i> and <i>Bacillus subtilis</i>. It carries erythromycin | ||
Latest revision as of 17:09, 17 October 2014
Vector
To get BioBricks in L. lactis, a compatible backbone is needed. For this,
we used a well known plasmid that is known to work in L. lactis, pIL253,
and made this BioBrick compatible. The plasmid is a shuttle vector that can be
used in L. lactis and Bacillus subtilis. It carries erythromycin
resistance as a selection marker and has a mRFP coding device between the
BioBrick prefix and suffix, so selection on inserts in this vector is
possible.
