Team:Groningen/Template/MODULE/projects/toolbox/vector

To get BioBricks in L. lactis, a compatible backbone is needed. For this, we used a well known plasmid that is known to work in L. lactis, pIL253, and made this BioBrick compatible. The plasmid is a shuttle vector that can be used in L. lactis and Bacillus subtilis. It carries erythromycin resistance as a selection marker and has a mRFP coding device between the BioBrick prefix and suffix, so selection on inserts in this vector is possible.