"
Team:OUC-China/Judging
From 2014.igem.org
| (33 intermediate revisions not shown) | |||
| Line 148: | Line 148: | ||
<li><a href="https://2014.igem.org/Team:OUC-China/Project_Future">Future</a></li> | <li><a href="https://2014.igem.org/Team:OUC-China/Project_Future">Future</a></li> | ||
<li><a href="https://2014.igem.org/Team:OUC-China/Project_Modeling">Modeling</a></li> | <li><a href="https://2014.igem.org/Team:OUC-China/Project_Modeling">Modeling</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:OUC-China/Project_Policy_Practise">Policy&Practice</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
| Line 154: | Line 155: | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li class="active"><a href="https://2014.igem.org/Team:OUC-China/Judging">Biobrick</a></li> | <li class="active"><a href="https://2014.igem.org/Team:OUC-China/Judging">Biobrick</a></li> | ||
| - | <li><a href="https://2014.igem.org/Team:OUC-China/Judging_Achievment"> | + | <li><a href="https://2014.igem.org/Team:OUC-China/Judging_Achievment">Achievement</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
| Line 160: | Line 161: | ||
<a href="#" class="dropdown-toggle" data-toggle="dropdown">SAFETY <b class="caret"></b></a> | <a href="#" class="dropdown-toggle" data-toggle="dropdown">SAFETY <b class="caret"></b></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| - | <li><a href="https://2014.igem.org/Team:OUC-China/Safety"> | + | <li><a href="https://2014.igem.org/Team:OUC-China/Safety">Biosafety</a></li> |
| - | <li><a href="https://2014.igem.org/Team:OUC-China/Safety_Lab_safety"> | + | <li><a href="https://2014.igem.org/Team:OUC-China/Safety_Lab_safety">Labsafety</a></li> |
| - | + | ||
</ul> | </ul> | ||
</li> | </li> | ||
| Line 191: | Line 191: | ||
</header> | </header> | ||
<div id="mainContent" style="width:1024px;background-color:#d2e9fd;padding:10px;margin:0 auto"> | <div id="mainContent" style="width:1024px;background-color:#d2e9fd;padding:10px;margin:0 auto"> | ||
| - | + | <style> | |
| - | + | .divide { border-top: 1px solid #103081; } | |
| + | </style> | ||
| + | <h1>Biobrick</h1> | ||
| + | <h4 class="text-primary divide">Origin of transfer for the RP4-plasmid nic region</h4> | ||
| + | <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439000">BBa_K1439000</a></p> | ||
| + | <p>Type: conjugation</p> | ||
| + | <p>Length:350bp</p> | ||
| + | <p>Description/Usage: OriTRP4,the RP4 plasmid nic region,is where the relaxosome nicks the plasmid and conjugative transfer by RP4 plasmid machinery begins.</p> | ||
| + | <p> </p> | ||
| + | <h4 class="text-primary divide">[OriTRP4]+[RFP]</h4> | ||
| + | <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439001">BBa_K1439001</a></p> | ||
| + | <p>Type: Composite</p> | ||
| + | <p>Length:1427bp</p> | ||
| + | <p>Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTRP4. Used to test plasmid mobility.</p> | ||
| + | <p></p> | ||
| + | <h4 class="text-primary divide">[OriTR]+[RFP]</h4> | ||
| + | <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439002">BBa_K1439002</a></p> | ||
| + | <p>Type: Composite</p> | ||
| + | <p>Length:1448bp</p> | ||
| + | <p>Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.</p> | ||
| + | <p></p> | ||
| + | <p class="text-primary"> </p> | ||
| + | <h4 class="text-primary divide">hEF1a_Cas9-VP16 MammoBlock</h4> | ||
| + | <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439003">BBa_K1179003</a></p> | ||
| + | <p>Type: Generator</p> | ||
| + | <p>Length: 5141bp</p> | ||
| + | <p>Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with guide RNA binding sites upstream.</p> | ||
| + | <h4 class="text-primary divide">TAT-H4</h4> | ||
| + | <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439004">BBa_K1439004</a></p> | ||
| + | <p>Type: Coding</p> | ||
| + | <p>Length:393bp</p> | ||
| + | <p>Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte.</p> | ||
| + | <h4 class="text-primary divide">TAT-PTD</h4> | ||
| + | <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439005">BBa_K1439005</a></p> | ||
| + | <p>Type: Coding</p> | ||
| + | <p>Length:33bp</p> | ||
| + | <p>Description/Usage: This part codes the expression of the TAT-PTD that is used in helping the plasmid transfect into the eukaryocyte.</p> | ||
| + | <h4 class="text-primary divide">Histone H4</h4> | ||
| + | <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439006">BBa_K1439006</a></p> | ||
| + | <p>Type: Coding</p> | ||
| + | <p>Length:312bp</p> | ||
| + | <p>Description/Usage: This part codes the expression of the Histone H4 that is used in combing and protecting the plasmid in bacterium.</p> | ||
| + | <h4 class="text-primary divide">CMV promoter</h4> | ||
| + | <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439007">BBa_K1439007</a></p> | ||
| + | <p>Type: Regulatory</p> | ||
| + | <p>Length:588bp</p> | ||
| + | <p>Description/Usage: We use CMV to improve the expression of TAT:H4 and the efficiency of transfection.</p> | ||
| + | <h4 class="text-primary divide">TAT-H4-B0015</h4> | ||
| + | <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439008">BBa_K1439008</a></p> | ||
| + | <p>Type: Composite</p> | ||
| + | <p>Length:530bp</p> | ||
| + | <p>Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte. It has a terminator and can be added different promoters.</p> | ||
| + | <h4 class="text-primary divide">Lysis device induced by L-arabinose</h4> | ||
| + | <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439009">BBa_K1439009</a></p> | ||
| + | <p>Type: Device</p> | ||
| + | <p>Length:1923bp</p> | ||
| + | <p>Description/Usage:This part encodes the expression of the lysis protein that is induced by L-arabinose mobility.</p> | ||
| + | <p></p> | ||
| + | <h4 class="text-primary divide">Lysis device induced by aTc</h4> | ||
| + | <p>ID: <a href="http://parts.igem.org/Part:BBa_K1439010">BBa_K1439010</a></p> | ||
| + | <p>Type: Device</p> | ||
| + | <p>Length:2738bp</p> | ||
| + | <p>Description/Usage:This is the lysis device using three genes from enterobacteria phage T4: lysozyme, holin, and antiholin.Tet promoter can control the lysis of bacteria. | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | </div> | ||
<script> | <script> | ||
$(document.body).scrollspy({ target: '#childNavigator' }); | $(document.body).scrollspy({ target: '#childNavigator' }); | ||
$('#childNavigator').affix({ offset: { top: 285 } }); | $('#childNavigator').affix({ offset: { top: 285 } }); | ||
</script> | </script> | ||
| + | <div class = "footer"> | ||
| + | <style> | ||
| + | .footer { padding: 10px; background-color: rgb(45,111,165); } | ||
| + | .footer > div { width: 418px; margin: 0 auto; padding-top: 50px; } | ||
| + | .footer > div > img { float: left; height: 100px; display: inline-block; position: relative; margin-left: 6px; margin-right: 6px; } | ||
| + | .footer > p { text-align: center; color: #ffffff } | ||
| + | .footer > p > a { color: #ffffff; } | ||
| + | </style> | ||
| + | <div> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/a/ad/OUC-China_Index_OrgLogo.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/e/ec/OUC-China_Index_OrgTitle.jpg" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/9/96/OUC-China_Foot_ouclogo.png" /> | ||
| + | <div style="clear:both"></div> | ||
| + | </div> | ||
| + | <p>contact us: <a href="mailto:oucigem@163.com">oucigem@163.com</a></p> | ||
| + | </div> | ||
<img class="anchor" onclick="document.documentElement.scrollTop = document.body.scrollTop = 0;" src="https://static.igem.org/mediawiki/2014/b/b6/ToTop.png"> | <img class="anchor" onclick="document.documentElement.scrollTop = document.body.scrollTop = 0;" src="https://static.igem.org/mediawiki/2014/b/b6/ToTop.png"> | ||
</html> | </html> | ||
Latest revision as of 03:07, 18 October 2014
Biobrick
Origin of transfer for the RP4-plasmid nic region
ID: BBa_K1439000
Type: conjugation
Length:350bp
Description/Usage: OriTRP4,the RP4 plasmid nic region,is where the relaxosome nicks the plasmid and conjugative transfer by RP4 plasmid machinery begins.
[OriTRP4]+[RFP]
ID: BBa_K1439001
Type: Composite
Length:1427bp
Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTRP4. Used to test plasmid mobility.
[OriTR]+[RFP]
ID: BBa_K1439002
Type: Composite
Length:1448bp
Description/Usage: This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.
hEF1a_Cas9-VP16 MammoBlock
ID: BBa_K1179003
Type: Generator
Length: 5141bp
Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with guide RNA binding sites upstream.
TAT-H4
ID: BBa_K1439004
Type: Coding
Length:393bp
Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte.
TAT-PTD
ID: BBa_K1439005
Type: Coding
Length:33bp
Description/Usage: This part codes the expression of the TAT-PTD that is used in helping the plasmid transfect into the eukaryocyte.
Histone H4
ID: BBa_K1439006
Type: Coding
Length:312bp
Description/Usage: This part codes the expression of the Histone H4 that is used in combing and protecting the plasmid in bacterium.
CMV promoter
ID: BBa_K1439007
Type: Regulatory
Length:588bp
Description/Usage: We use CMV to improve the expression of TAT:H4 and the efficiency of transfection.
TAT-H4-B0015
ID: BBa_K1439008
Type: Composite
Length:530bp
Description/Usage: This part codes the expression of the fusion protein TAT-H4 that is used in combing and protecting the plasmid in bacterium and helping the plasmid transfect into the eukaryocyte. It has a terminator and can be added different promoters.
Lysis device induced by L-arabinose
ID: BBa_K1439009
Type: Device
Length:1923bp
Description/Usage:This part encodes the expression of the lysis protein that is induced by L-arabinose mobility.
Lysis device induced by aTc
ID: BBa_K1439010
Type: Device
Length:2738bp
Description/Usage:This is the lysis device using three genes from enterobacteria phage T4: lysozyme, holin, and antiholin.Tet promoter can control the lysis of bacteria.
contact us: oucigem@163.com
