Team:SF Bay Area DIYbio/Notebook

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:SF_Bay_Area_DIYbio/Notebook&amp;action=edit" style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:SF_Bay_Area_DIYbio/Notebook&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/Parts"style="color:#000000"> Parts</a></td>
<a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/Parts"style="color:#000000"> Parts</a></td>
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<a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/Modeling"style="color:#000000"> Modeling</a></td>
 
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<a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/Safety"style=" color:#000000"> Safety </a></td>
<a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/ELSEI"style=" color:#000000">Ethical, Legal, Societal, Environmental Issues</a></td>
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<a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/Attributions"style="color:#000000"> Attributions </a></td>
<a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/Attributions"style="color:#000000"> Attributions </a></td>
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<a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/Future_Plans"style="color:#000000"> Future Plans </a></td>
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<p> &nbsp; </p>
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<p><b>Notebook</b></p>
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<p><b><font size="5">Notebook</font></b></p><br>
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<p><b>General Protocols</b></p>
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<p><b><font size="4">General Protocols</font></b></p>
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   <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/ElectraCloningProtocol"> Electra Cloning Protocol </a><br>
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   <li><a href="https://www.dna20.com/products/expression-vectors/electra-system#2"> Electra Cloning Protocol </a><br>
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   <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/DNAhandling"> DNA Handling Protocol </a><br>
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   </li><li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/DNAhandling"> DNA Handling Protocol </a><br>
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   <li>Media:Zymo E. coli competent cells transformation protocol.pdf<br>
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   </li><li><a href="https://static.igem.org/mediawiki/2014/1/1e/Zymo_E._coli_competent_cells_transformation_protocol.pdf">Media:Zymo E. coli competent cells transformation protocol</a><br>
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   <li>Media:D4025i.pdf (Zyppy plasmid midiprep protocol)<br>
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   </li><li><a href="https://static.igem.org/mediawiki/2014/0/0e/Zyppy_plasmid_midiprep_kit_protocol.pdf">Media:D4025i (Zyppy plasmid midiprep protocol)</a><br>
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   <li>Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf <br>
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   </li><li><a href="https://static.igem.org/mediawiki/2014/4/4d/Clontech_UTF-8%27en-us%27Quick_Easy_Yeast_Transformation_Mix_Protocol-At-A-Glance_040214.pdf">Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214</a> <br>
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   <li>Media:Sequencing_Analysis_using_Serial_Cloner_2_16Oct2014.pdf‎<br></ul>
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   </li><li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/MediaSequencingAnalysis">  Media:Sequencing_Analysis_using_Serial_Cloner_2_16Oct2014‎</a><br></li></ul>
<br>
<br>
<p>General Documentation Information</p><ul>
<p>General Documentation Information</p><ul>
     <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/NotebookDocumentation">Real Vegan Cheese Notebook Documentation Guidelines</a><br>
     <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/NotebookDocumentation">Real Vegan Cheese Notebook Documentation Guidelines</a><br>
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     </ul>
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<p><b><font size="4">Experiments</font></b></p>
<p><b>June 2014</b></p><ul>
<p><b>June 2014</b></p><ul>
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     <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/NoteBookJune1">CR_062214_humanKappaCasein_001</a> <br></ul></p><br>
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     <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/NoteBookJune1">CR_062214_humanKappaCasein_001<b>22 June 2014</b></a> <br></li></ul><p></p><br>
<p><b>August 2014</b></p><ul>
<p><b>August 2014</b></p><ul>
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     <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/AugustCloning">CLONE_082514_humKappaCasein(Kex+)</a><br>
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     <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/AugustCloning">CLONE_082514_humKappaCasein(Kex+)<b>25 Aug 2014</b></a><br>
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     <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/AugustGrowthCurve">Growth Curve Experiment</a><br></ul></p><br>
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     </li><li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/AugustGrowthCurve">Growth Curve Experiment <b>22 Aug 2014</b></a><br></li></ul><p></p><br>
<p><b>September 2014</b></p><ul>
<p><b>September 2014</b></p><ul>
     <li><a href="https://2014.igem.org/cloning7caesin">Cloning 7 casein gBlocks, <b>12 Sep 2014</b></a><br>
     <li><a href="https://2014.igem.org/cloning7caesin">Cloning 7 casein gBlocks, <b>12 Sep 2014</b></a><br>
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     <li><a href="https://2014.igem.org/troubleshooting12.9">Troubleshooting failed transformation of P.FAKS.humBeta.S on <b>12 Sept. 2014</b></a><br>
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     </li><li><a href="https://2014.igem.org/troubleshooting12.9">Troubleshooting failed transformation of P.FAKS.humBeta.S on <b>12 Sept. 2014</b></a><br>
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     <li><a href="https://2014.igem.org/CloningBovineBetaCaesinSept">Cloning bovine beta casein, Fam20C (Kex + & -), from <b>27Sep2014</b></a><br>
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     </li><li><a href="https://2014.igem.org/CloningBovineBetaCaesinSept">Cloning bovine beta casein, Fam20C (Kex + &amp; -), from <b>27Sep2014</b></a><br>
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     <li><a href="https://2014.igem.org/Submitting10CaesinSept">Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing <b>30Sep2014</b></a><br></ul></p><br>
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     </li><li><a href="https://2014.igem.org/Submitting10CaesinSept">Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing <b>30Sep2014</b></a><br></li></ul><p></p><br>
<p><b>October 2014</b></p><ul>
<p><b>October 2014</b></p><ul>
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    <li><a href="https://2014.igem.org/InitialYeastStudyOct">Initial studies of yeast strains' growth conditions and troubleshooting <b>Oct.2</b></a><br>
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<!-- <li><a href="https://2014.igem.org/InitialYeastStudyOct">Initial studies of yeast strains' growth conditions and troubleshooting <b>Oct.2</b></a><br>-->
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     <li><a href="https://2014.igem.org/RepeadMidiprepsOct">Repeat Midipreps to select new clones for sequencing for failed sequences from <b>Oct. 2</b></a><br>
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     </li><li><a href="https://2014.igem.org/RepeadMidiprepsOct">Repeat Midipreps to select new clones for sequencing for failed sequences from <b>Oct. 2</b></a><br>
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     <li><a href="https://2014.igem.org/CloningHumanAlphaS1Oct">Cloning human alpha casein S1, <b>04Oct2014</b></a><br>
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     </li><li><a href="https://2014.igem.org/CloningHumanAlphaS1Oct">Cloning human alpha casein S1, <b>04Oct2014</b></a><br>
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    <li><a href="https://2014.igem.org/CloningBovineKappaBetaOct">Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit <b>08Oct2014</b></a><br>
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<!--</li><li><a href="https://2014.igem.org/CloningBovineKappaBetaOct">Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit <b>08Oct2014</b></a><br>-->
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     <li><a href="https://2014.igem.org/SubmittingPlasmidSequencingOct">Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing <b>10Oct2014</b></a><br>
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     </li><li><a href="https://2014.igem.org/SubmittingPlasmidSequencingOct">Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing <b>10Oct2014</b></a><br>
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    <li><a href="https://2014.igem.org/CloningBovineKappaBetaBioBrickOct">Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit <b>10Oct2014</b></a><br>
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<!--    </li><li><a href="https://2014.igem.org/CloningBovineKappaBetaBioBrickOct">Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit <b>10Oct2014</b></a><br>-->
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     <li><a href="https://2014.igem.org/CloningBovineBetaOct">Cloning bovineBeta into pSB1C3 Biobrick using NEB biobrick kit <b>13Oct2014</b></a><br>
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     <!--</li><li><a href="https://2014.igem.org/CloningBovineBetaOct">Cloning bovineBeta into pSB1C3 Biobrick using NEB biobrick kit <b>13Oct2014</b></a><br>-->
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     <li><a href="https://2014.igem.org/SubmittingFam20COct">Submitting FAM20C (Kex + & Kex -) for sequencing with new internal primers <b>14Oct2014</b></a><br>
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     </li><li><a href="https://2014.igem.org/SubmittingFam20COct">Submitting FAM20C (Kex + &amp; Kex -) for sequencing with new internal primers <b>14Oct2014</b></a><br>
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    <li><a href="https://2014.igem.org/SubmittingFam20CkexOct">Submitting FAM20C Kex + clone 9A and bovineKappa and bovineBeta: pSB1C3 Biobrick for sequencing <b>15Oct2014</b></a><br><br></ul></p>
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<!--    </li><li><a href="https://2014.igem.org/SubmittingFam20CkexOct">Submitting FAM20C Kex + clone 9A and bovineKappa and bovineBeta: pSB1C3 Biobrick for sequencing <b>15Oct2014</b></a>-->
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Notebook


General Protocols


General Documentation Information


Experiments

June 2014


August 2014


September 2014


October 2014