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Team:UT-Dallas/Notebook/6-10
From 2014.igem.org
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| - | + | June 10,2014 we mini prepped i5.9.1 which was RBS and also i5.9.2 which was promoter. We then did a digestion and gel electrophoresis and Purification for the promoter using a 2 log ladder as seen in figure:6.10.1. Finally we Ligated and Transformed P-Rbs-eCFP,P-Rbs-YG,P-Rbs-eYFP,P-Rbs-redch., RBS-bluechromo., and a negative control. </p> | |
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| + | <LI>>Mini Prep | ||
| + | <LI> Digestion and Gel Electrophoresis and Purification | ||
| + | <LI> Ligate and Transform | ||
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| + | <img src="https://static.igem.org/mediawiki/2014/e/ec/July_10.jpg" style="border-width: 0px; display: inline; margin-left: 0px; margin-top: 0px;" height="400px"> | ||
| + | <br> Figure:6.10.1 Gel with 2 log ladder promoter band | ||
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Latest revision as of 00:17, 18 October 2014
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Tuesday, June 10th, 2014
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June 10,2014 we mini prepped i5.9.1 which was RBS and also i5.9.2 which was promoter. We then did a digestion and gel electrophoresis and Purification for the promoter using a 2 log ladder as seen in figure:6.10.1. Finally we Ligated and Transformed P-Rbs-eCFP,P-Rbs-YG,P-Rbs-eYFP,P-Rbs-redch., RBS-bluechromo., and a negative control. |
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Figure:6.10.1 Gel with 2 log ladder promoter band |
