Team:UT-Dallas/Notebook/6-10

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Tuesday, June 10th, 2014

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June 10,2014 we mini prepped i5.9.1 which was RBS and also i5.9.2 which was promoter. We then did a digestion and gel electrophoresis and Purification for the promoter using a 2 log ladder as seen in figure:6.10.1. Finally we Ligated and Transformed P-Rbs-eCFP,P-Rbs-YG,P-Rbs-eYFP,P-Rbs-redch., RBS-bluechromo., and a negative control.

  • >Mini Prep
  • Digestion and Gel Electrophoresis and Purification
  • Ligate and Transform


    • Figure:6.10.1 Gel with 2 log ladder promoter band

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