Team:Cambridge-JIC/Guide/Materials/Checklist

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=Checklist=
=Checklist=
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This checklist contains everything you will need for setting up transformation.
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This checklist contains the minimum you will need for setting up transformation.
==DNA==
==DNA==
-
- A [[Team:Cambridge-JIC/Guide/Constructs/AgrobacteriumVectors| helper plasmid]] for [[Team:Cambridge-JIC/Guide/Transformation|Transformation]
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- A [[Team:Cambridge-JIC/Guide/Constructs/AgrobacteriumVectors| helper plasmid]] for [[Team:Cambridge-JIC/Guide/Transformation|Transformation]]
 +
 
- Your [[Team:Cambridge-JIC/Guide/Constructs|T-DNA plasmid]] for transformation
- Your [[Team:Cambridge-JIC/Guide/Constructs|T-DNA plasmid]] for transformation
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==[[Team:Cambridge-JIC/Guide/Materials/Strains |Bacteria]]==
==[[Team:Cambridge-JIC/Guide/Materials/Strains |Bacteria]]==
- Competent E-Coli (chemically/electro)
- Competent E-Coli (chemically/electro)
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- [[Team:Cambridge-JIC/Guide/Transformation/PreparingElectrocompetentAgro | Electrocompetent agrobacteria]]
- [[Team:Cambridge-JIC/Guide/Transformation/PreparingElectrocompetentAgro | Electrocompetent agrobacteria]]
==Marchantia==
==Marchantia==
- [[Team:Cambridge-JIC/Guide/Care/PreparingSpores| Sporangia]]
- [[Team:Cambridge-JIC/Guide/Care/PreparingSpores| Sporangia]]
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OR
OR
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- Male and female Marchantia polymorpha [from the garden]
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- Male and female Marchantia polymorpha [from the garden], for [[Team:Cambridge-JIC/Guide/Care/SexualReproduction|growing spores]]
==Miscellaneous==
==Miscellaneous==
- [[Team:Cambridge-JIC/Guide/Materials/Media|Gamborg's B5 salt mix]]
- [[Team:Cambridge-JIC/Guide/Materials/Media|Gamborg's B5 salt mix]]
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- LB/SOC for bacterial transformation and growth
- LB/SOC for bacterial transformation and growth
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- 100mM Acetosyringone  
- 100mM Acetosyringone  
==Equipment==
==Equipment==
- Ventilated cupboard/room/box under full illumination [larger space = more transformations]
- Ventilated cupboard/room/box under full illumination [larger space = more transformations]
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Latest revision as of 15:29, 17 October 2014

Cambridge iGEM 2014


Contents

Checklist

This checklist contains the minimum you will need for setting up transformation.

DNA

- A helper plasmid for Transformation

- Your T-DNA plasmid for transformation

Bacteria

- Competent E-Coli (chemically/electro)

- Electrocompetent agrobacteria

Marchantia

- Sporangia

OR

- Male and female Marchantia polymorpha [from the garden], for growing spores

Miscellaneous

- Gamborg's B5 salt mix

- LB/SOC for bacterial transformation and growth

- 100mM Acetosyringone

Equipment

- Ventilated cupboard/room/box under full illumination [larger space = more transformations]