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From 2014.igem.org

Preparing Electrocompetent Agrobacteria

Many protocols for preparing electrocompetent agrobacteria can be found online, and are roughly the same. The protocol given in this guide for electroporation uses 50ul of agrobacteria cells per transformation- so you should aliquot appropriately.

For example: http://www.plantsci.cam.ac.uk/research/jillharrison/protocols/cloning/preparation-of-agrobacterium-electrocompetent.pdf

Although electrocompetent agrobacteria are available for purchase, you will want to create a stock of electrocompetent agrobacteria containing the Ti plasmid, since double transformation can lead to low efficiencies.

After you've prepared these cells, plate an untransformed batch onto selective plates with the antibiotic you are planning on transforming them with. Make sure that no colonies appear!

Next steps

See transforming Agrobacterium for how to perform the electroporation.