Team:Bielefeld-CeBiTec/Notebook/Journal/Isobutanol/Aug

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                       <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification</a> (as described before)</li>
                       <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification</a> (as described before)</li>
              <ul>
              <ul>
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                         <li>We used pSB1C3-RFP instead of pSB1C3-CFP as template for backbone amplification</li>
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                         <li>We used pSB1C3-RFP (<a href="http://parts.igem.org/Part:BBa_J04450" target="_blank">J04450</a>) instead of pSB1C3-CFP as template for backbone amplification</li>
        <li>Annealing temperature: 65 °C</li>
        <li>Annealing temperature: 65 °C</li>
        <li>Bands as expected (~ 2200 bp)</li>
        <li>Bands as expected (~ 2200 bp)</li>
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  <li>This week the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Organisms#L.lactis" target="_blank"> <i>Lactococcus lactis</i></a> arrived. We tried to amplify it's <i>adhA</i>.</li>
  <li>This week the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Organisms#L.lactis" target="_blank"> <i>Lactococcus lactis</i></a> arrived. We tried to amplify it's <i>adhA</i>.</li>
                   <ul>
                   <ul>
-
                    PCR:
+
                  <ul>
 +
<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#DNAisolation" target="_blank">DNA isolation</a> of <i>Lactococcus lactis</i></li>
 +
</ul>
 +
 
<ul>
<ul>
<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification</a> of the <i>adhA</i> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rev-pBS1C3-adhA" target="_blank">rev-pBS1C3-adhA</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw-pSB1C3_adhA" target="_blank">fw-pSB1C3_adhA</a>) and the Backbone out of psB1K3-<i>RFP</i> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rev-adhA_pSB1C3" target="_blank">rev-adhA_pSB1C3</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw-adhA-pSB1C3" target="_blank">fw-adhA-pSB1C3</a>)</li>
<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification</a> of the <i>adhA</i> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rev-pBS1C3-adhA" target="_blank">rev-pBS1C3-adhA</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw-pSB1C3_adhA" target="_blank">fw-pSB1C3_adhA</a>) and the Backbone out of psB1K3-<i>RFP</i> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rev-adhA_pSB1C3" target="_blank">rev-adhA_pSB1C3</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw-adhA-pSB1C3" target="_blank">fw-adhA-pSB1C3</a>)</li>

Latest revision as of 16:49, 16 October 2014


August

  • alsS, ilvC, ilvD, kivD and backbone pSB1K3
    • This week we tried to begin from zero again with optimized conditions and new competent cells.
      Therefor we prepared new plasmids from alsS, ilvC, ilvD, kivD and the backbone pSB1K3-RFP