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Team:Caltech/week12
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<a id='home' href="https://2014.igem.org/Team:Caltech"style="color:#000000">Home </a> </td> | <a id='home' href="https://2014.igem.org/Team:Caltech"style="color:#000000">Home </a> </td> | ||
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<table> | <table> | ||
| - | <tr><td colspan= | + | <tr> <td bgColor=#FFFFFF colspan = 3 height = 60px> <font size = +5> <center>Notebook </center> </td> </tr> |
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<b><a href='week11'>Week 11</a></b><br><br> | <b><a href='week11'>Week 11</a></b><br><br> | ||
<b><font size=+1>Week 12</font></b><br><br> | <b><font size=+1>Week 12</font></b><br><br> | ||
| + | <b><a href='week13'>Week 13</a></b><br><br> | ||
| + | <b><a href='week14'>Week 14</a></b><br><br> | ||
| + | <b><a href='week15'>Week 15</a></b><br><br> | ||
<!-- links end here --> | <!-- links end here --> | ||
</td> | </td> | ||
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<tr><td colspan=2><h1>Week Twelve</h1></td></tr> | <tr><td colspan=2><h1>Week Twelve</h1></td></tr> | ||
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| + | <tr> | ||
| + | <td width="25%" valign="top"> | ||
| + | <h4>Sunday, 8/31/14</h4> | ||
| + | </td> | ||
| + | <td valign="top"> | ||
| + | <b>Export Systems</b> | ||
| + | <br>Redo of cloning of agr system: | ||
| + | <ul><li>Ran colony PCR on 10 picked overnight colonies.</li> | ||
| + | <li>colony PCR products were then run on a gel.</li> | ||
| + | <li>5 LB-CARB liquid cultures were set up and incubated overnight.</li> | ||
| + | </ul> | ||
| + | De-FLAGging pTG005 for LC/MS analysis: | ||
| + | <ul><li>Ran colony PCR on 10 picked overnight colonies.</li> | ||
| + | <li>colony PCR products were then run on a gel.</li> | ||
| + | <li>3 LB-CARB liquid cultures were set up and incubated overnight.</li> | ||
| + | </ul> | ||
| + | </td> | ||
| + | </tr> | ||
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| + | <tr><td bgColor="#777777" colspan="2" height="1px"></td></tr> | ||
<tr> | <tr> | ||
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<td valign="top"> | <td valign="top"> | ||
| + | <b>Export Systems</b> | ||
| + | <br>Redo of cloning of agr system: | ||
| + | <ul><li>Miniprepped the 5 liquid cultures from yesterday. Stored the plasmids in the fridge.</li></ul> | ||
| + | De-FLAGging pTG005 for LC/MS analysis: | ||
| + | <ul><li>Miniprepped the 3 liquid cultures from yesterday. Stored the plasmids in the fridge.</li></ul> | ||
| + | <b>lamBCDA & fsrABC Reception Systems</b> | ||
<ul> | <ul> | ||
</ul> | </ul> | ||
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<td valign="top"> | <td valign="top"> | ||
| + | <b>lamBCDA & fsrABC Reception Systems</b> | ||
<ul> | <ul> | ||
| + | </ul> | ||
| + | <b>agrBCDA Reception System and Combinatorial Promoters</b> | ||
| + | <ul><li>Ran colony PCR using liquid cultures of Gibson colonies inoculated on Friday, in hopes of obtaining clearer gel results</li> | ||
</ul> | </ul> | ||
</td> | </td> | ||
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</td> | </td> | ||
<td valign="top"> | <td valign="top"> | ||
| + | <b>Export Systems</b> | ||
| + | <ul><li>Shipped samples for both agr-recloning subproject (2*5=10 samples) & pTG005-unFLAGging subproject (2*3=6 samples) out for sequencing.</li> | ||
| + | </ul> | ||
| + | <b>lamBCDA & fsrABC Reception Systems</b> | ||
<ul> | <ul> | ||
</ul> | </ul> | ||
| - | < | + | <b>agrBCDA Reception System and Combinatorial Promoters</b> |
| + | <ul><li>Ran gel of colony PCR products from yesterday </li> | ||
| + | <li>Gel still yielded unfavorable results</li> | ||
| + | <li>Planning to clone using Golden Gate assembly </li> | ||
| + | </ul> | ||
</td> | </td> | ||
</tr> | </tr> | ||
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</td> | </td> | ||
<td valign="top"> | <td valign="top"> | ||
| - | <ul> | + | <b>Export Systems</b> |
| + | <ul><li>Sequencing data appears to confirm successful unFLAGging of pTG005.</li> | ||
</ul> | </ul> | ||
</td> | </td> | ||
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</td> | </td> | ||
<td valign="top"> | <td valign="top"> | ||
| - | < | + | </td> |
| - | </ | + | </tr> |
| + | |||
| + | <tr><td bgColor="#777777" colspan="2" height="1px"></td></tr> | ||
| + | |||
| + | <tr> | ||
| + | <td width="25%" valign="top"> | ||
| + | <h4>Saturday, 9/6/14</h4> | ||
| + | </td> | ||
| + | <td valign="top"> | ||
</td> | </td> | ||
</tr> | </tr> | ||
Latest revision as of 01:02, 15 September 2014
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