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Team:TU Darmstadt/Notebook/Methods/hydrochloric acid extraction
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| - | <!--TYPO3SEARCH_begin--><div id="c246" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">37% | + | <!--TYPO3SEARCH_begin--><div id="c246" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">37% Hydrochloric Acid Extraction</h1></div><div class="csc-textpic-text"><div><p><b>Materials: </b></p></div><div><p><span style="font-size: 14px;">- 37% hydrochloric acid</span><br /><span style="font-size: 14px;">- Centrifuge</span><br /><span style="font-size: 14px;">- Tubes</span></p></div><div></div><div><p><b>Procedure:</b></p></div><div><p>- Spin down the bacterial solution at 4500g for 10 minutes.</p></div><div><p>- Remove culture media, weigh the remaining pellet and acetify with the same amount of 37% hydrochloric acid. </p></div><div><p>- Spin down the resolved pellet at 13,3g for 10 minutes and Remove supernatant.</p></div><div><p>The anthocyanidin is present in the organic and the aqueous phase; the color can be changed by adjusting the pH-value. </p></div><div></div></div></div><!--TYPO3SEARCH_end--> |
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Latest revision as of 00:49, 18 October 2014
37% Hydrochloric Acid Extraction
Materials:
- 37% hydrochloric acid
- Centrifuge
- Tubes
Procedure:
- Spin down the bacterial solution at 4500g for 10 minutes.
- Remove culture media, weigh the remaining pellet and acetify with the same amount of 37% hydrochloric acid.
- Spin down the resolved pellet at 13,3g for 10 minutes and Remove supernatant.
The anthocyanidin is present in the organic and the aqueous phase; the color can be changed by adjusting the pH-value.
