Team:TU Darmstadt/Notebook/Methods/37 hydrochloric acid

From 2014.igem.org

(Difference between revisions)
(Created page with "<div id="contentWrap" class="container_24"> <div id="breadcrumbs" class="grid_24"> <p>Sie sind hier:  <a href="index.php?id=23" >wiki</a> › <a href="i...")
 
(4 intermediate revisions not shown)
Line 1: Line 1:
-
<div id="contentWrap" class="container_24">
+
{{:Team:TU_Darmstadt/Template}}
-
<div id="breadcrumbs" class="grid_24">
+
 
-
<p>Sie sind hier:&nbsp; <a href="index.php?id=23" >wiki</a> &rsaquo;&nbsp;<a href="index.php?id=32" >Notebook</a> &rsaquo;&nbsp;<a href="index.php?id=54" >Methods</a> &rsaquo;&nbsp;<span class="current"><a href="index.php?id=132" >37% hydrochloric acid extraction</a></span></p>
+
 
-
</div>
+
<html>
 +
 
<div id="leftNavi" class="grid_5">
<div id="leftNavi" class="grid_5">
 +
<nav>
<nav>
-
<ul class="menu"><li class="first"><a href="index.php?id=160" >Home</a></li><li><a href="index.php?id=28" >Project</a></li><li><a href="index.php?id=29" >Results</a></li><li><a href="index.php?id=30" >Policy & Practices</a></li><li><a href="index.php?id=31" >Achievements</a></li><li class="active"><a href="index.php?id=32" >Notebook</a><ul class="menu2"><li class="first"><a href="index.php?id=53" >Labjournal</a></li><li><a href="index.php?id=73" >Materials</a></li><li class="active last"><a href="index.php?id=54" >Methods</a><ul class="menu3"><li class="first"><a href="index.php?id=84" >Agarose gel electrophoresis</a></li><li><a href="index.php?id=85" >Dephosphorylation</a></li><li><a href="index.php?id=86" >DNA ligation</a></li><li><a href="index.php?id=87" >DNA quantification with NanoDrop</a></li><li><a href="index.php?id=89" >PCR purification</a></li><li><a href="index.php?id=90" >PCR with Pfu polymerase</a></li><li><a href="index.php?id=91" >Plasmid preparation</a></li><li><a href="index.php?id=92" >Restriction digest</a></li><li><a href="index.php?id=93" >SDS-PAGE</a></li><li><a href="index.php?id=94" >Bacterial cell culture</a></li><li><a href="index.php?id=95" >Cell counting/plating</a></li><li><a href="index.php?id=96" >Chemically competent cells</a></li><li><a href="index.php?id=97" >Colony PCR with Taq polymerase</a></li><li><a href="index.php?id=98" >Glycerine stocks</a></li><li><a href="index.php?id=99" >Heat shock transformation</a></li><li><a href="index.php?id=100" >Protein expression</a></li><li class="active"><a href="index.php?id=132" >37% hydrochloric acid extraction</a></li><li><a href="index.php?id=131" >Dichloromethane extraction</a></li><li><a href="index.php?id=134" >Methanol extraction</a></li><li class="last"><a href="index.php?id=135" >Ethanol extraction</a></li></ul></li></ul></li><li><a href="index.php?id=33" >Team</a></li><li class="last"><a href="index.php?id=34" >Gallery</a></li></ul>
+
<ul class="menu"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt" >Home</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Project" >Project</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results" >Results</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/PolicyandPractices" >Policy & Practices</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Achievements" >Achievements</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook" >Notebook</a><ul class="menu2"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal" >Labjournal</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials" >Materials</a></li><li class="active last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods" >Methods</a><ul class="menu3"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Agarose_gel_electrophoresis" >Agarose gel electrophoresis</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Dephosphorylation" >Dephosphorylation</a></li><li ><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/DNA_ligation" >DNA ligation</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/DNA_quantification_with_NanoDrop" >DNA quantification with NanoDrop</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/PCR_purification" >PCR purification</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/PCR_with_Pfu_polymerase" >PCR with Pfu polymerase</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Plasmid_preparation" >Plasmid preparation</a></li ><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Restriction_digest" >Restriction digest</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/SDS-PAGE" >SDS-PAGE</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Bacterial_cell_culture" >Bacterial cell culture</a></li><li ><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Cell_counting_plating" >Cell counting/plating</a></li><li ><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Chemically_competent_cells" >Chemically competent cells</a></li><li ><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Colony_PCR_with_Taq_polymerase" >Colony PCR with Taq polymerase</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Glycerine_stocks" >Glycerine stocks</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Heat_shock_transformation" >Heat shock transformation</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Protein expression" >Protein expression</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/hydrochloric_acid_extraction" >37% hydrochloric acid extraction</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Dichloromethane_extraction" >Dichloromethane extraction</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Methanol_extraction" >Methanol extraction</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Ethanol_extraction" >Ethanol extraction</a></li></ul></li></ul></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Team" >Team</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Gallery" >Gallery</a></li></ul>
</nav>
</nav>
</div>
</div>
<div id="wikicontent" class="grid_19">
<div id="wikicontent" class="grid_19">
-
<!--TYPO3SEARCH_begin--><div id="c246" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">37% hydrochloric acid extraction</h1></div><div class="csc-textpic-text"><div><p><b>Materials:&nbsp;</b></p></div><div><p><span style="font-size: 12px;">- 37% hydrochloric acid</span><br /><span style="font-size: 12px;">- Centrifuge</span><br /><span style="font-size: 12px;">- Tubes</span></p></div><div></div><div><p><b>Procedure:</b></p></div><div><p>Spin down the bacterial solution at 4500g for 10 minutes.</p></div><div><p>Remove culture media, weigh the remaining pellet and acetify with the same amount of 37% hydrochloric acid.&nbsp;</p></div><div><p>Spin down the resolved pellet at 13,3g for 10 minutes and Remove supernatant.</p></div><div><p>The anthocyanidin is present in the organic and the aqueous phase; the color can be changed by adjusting the pH-value.&nbsp;</p></div><div></div></div></div><!--TYPO3SEARCH_end-->
+
<!--TYPO3SEARCH_begin--><div id="c246" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">37% hydrochloric acid extraction</h1></div><div class="csc-textpic-text"><div><p><b>Materials:&nbsp;</b></p></div><div><p><span style="font-size: 12px;">- 37% hydrochloric acid</span><br /><span style="font-size: 12px;">- Centrifuge</span><br /><span style="font-size: 12px;">- Tubes</span></p></div><div></div><div><p><b>Procedure:</b></p></div><div><p>- Spin down the bacterial solution at 4500g for 10 minutes.</p></div><div><p>- Remove culture media, weigh the remaining pellet and acetify with the same amount of 37% hydrochloric acid.&nbsp;</p></div><div><p>- Spin down the resolved pellet at 13,3g for 10 minutes and Remove supernatant.</p></div><div><p>The anthocyanidin is present in the organic and the aqueous phase; the color can be changed by adjusting the pH-value.&nbsp;</p></div><div></div></div></div><!--TYPO3SEARCH_end-->
</div>
</div>
Line 60: Line 62:
</div>
</div>
</div> <!--ende contentWrap-->
</div> <!--ende contentWrap-->
 +
</html>

Latest revision as of 19:36, 17 October 2014

Home


37% hydrochloric acid extraction

Materials: 

- 37% hydrochloric acid
- Centrifuge
- Tubes

Procedure:

- Spin down the bacterial solution at 4500g for 10 minutes.

- Remove culture media, weigh the remaining pellet and acetify with the same amount of 37% hydrochloric acid. 

- Spin down the resolved pellet at 13,3g for 10 minutes and Remove supernatant.

The anthocyanidin is present in the organic and the aqueous phase; the color can be changed by adjusting the pH-value.