Team:Tsinghua/Project

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      <li><a href="https://2014.igem.org/Team:Tsinghua/Main_page">MAIN PAGE</a></li>
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      <li><a href="https://2014.igem.org/Team:Tsinghua/Introduction">INTRODUCTION</a>
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          <li><a href="https://2014.igem.org/Team:Tsinghua/Introduction">Overview</a></li>     
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          <li><a href="https://2014.igem.org/Team:Tsinghua/Introduction/Diabetes">Diabetes Type I</a></li>
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          <li><a href="https://2014.igem.org/Team:Tsinghua/Introduction/Gene_therapy">Gene Therapy</a></li>
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      <li><a href="https://2014.igem.org/Team:Tsinghua/Project">PROJECT</a>
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<li><a href="https://2014.igem.org/Team:Tsinghua/Project">Overview</a></li>     
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<li><a href="https://2014.igem.org/Team:Tsinghua/Project/Virus">The Virus</a></li>     
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<li><a href="https://2014.igem.org/Team:Tsinghua/Project/Drug">The Drug</a></li>
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<li><a href="https://2014.igem.org/Team:Tsinghua/Project/Microbe">The Microbe</a></li>
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<li><a href="https://2014.igem.org/Team:Tsinghua/Project/Cocktail">The Cocktail</a></li>
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      <li><a href="https://2014.igem.org/Team:Tsinghua/Modeling">Modeling</a></li>
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      <li><a href="https://2014.igem.org/Team:Tsinghua/Parts">Parts</a></li>
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      <li><a href="https://2014.igem.org/Team:Tsinghua/Safety">SAFETY</a></li>
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      <li><a href="https://2014.igem.org/Team:Tsinghua/Notebook">NOTEBOOK</a>
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        <li><a href="https://2014.igem.org/Team:Tsinghua/Notebook">OVERVIEW</a></li>
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        <li><a href="https://2014.igem.org/Team:Tsinghua/Notebook/Protocol">PROTOCOL</a></li>
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        <li><a href="https://2014.igem.org/Team:Tsinghua/Notebook/Lablog">LABLOG</a></li>
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      <li><a href="https://2014.igem.org/Team:Tsinghua/Human_practice">HUMAN PRACTICE</a>
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  <li><a href="https://2014.igem.org/Team:Tsinghua/Human_practice">OVERVIEW</a></li>
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          <li><a href="https://2014.igem.org/Team:Tsinghua/Human_practice/Gallery">GALLERY</a></li>
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          <li><a href="https://2014.igem.org/Team:Tsinghua/Human_practice/Outreach">OUTREACH</a></li>
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          <li><a href="https://2014.igem.org/Team:Tsinghua/Human_practice/Video">VIDEO</a></li>         
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      <li><a href="https://2014.igem.org/Team:Tsinghua/Team">TEAM</a></li>
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    <h1>Project: Overview</h1>
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<h2>Diabeat: a gene therapy for Type I Diabetes Mellitus</h2>
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<p><i><b> Type I diabetes mellitus </b></i> (T1DM) affects more than 17 million people worldwide, and current treatments for T1DM, known as <i><b> insulin therapy </b></i>, require continued insulin injection, diet control, and constant monitoring of blood sugar. While <i><b> gene therapy </b></i> methods that restore insulin production in non-pancreatic cells might provide a one-shot cure for T1DB. We propose a <i><b> gene therapy </b></i> for T1DM using an <i><b> adeno-associated viral </b></i> (AAV) vector that transfects somatic cells with an insulin gene controlled by a glucose-sensitive promoter, thus potentially able to restore <i><b> glucose-regulated </b></i> insulin production in diabetic patients. Preliminary testing will be conducted on cell lines to assess the efficiency of the therapy.</p>
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<p>With the aim of restoring insulin production in T1DM patients, we propose to genetically modify non-beta cells to produce insulin at high glucose concentration. An engineered AAV vector will deliver the insulin production system into 293 cell lines to be tested. The insulin gene has been cloned and modified to allow for efficient proteolytic cleavage in non-beta cells (furin). In initial tests fluorescent proteins was be used instead of insulin as a reporter, and the effects are assessed using FACS and microscopy.</p>
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<p>In short, our project is based on <b><i>one</i> Gene Therapy Platform</b> and <b><i>three</i></b> independently functioning <b>Insulin Production Systems</b> that would provide a care-free cure for Diabetes Type I.</p>  
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<u><p>Click on any of the icons below to read more about our "one platform and three systems".</p></u>
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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Tsinghua/Project&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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Latest revision as of 22:11, 17 October 2014

Team Tsinghua 2014 Return to iGEM 2014

Project: Overview

Diabeat: a gene therapy for Type I Diabetes Mellitus

Type I diabetes mellitus (T1DM) affects more than 17 million people worldwide, and current treatments for T1DM, known as insulin therapy , require continued insulin injection, diet control, and constant monitoring of blood sugar. While gene therapy methods that restore insulin production in non-pancreatic cells might provide a one-shot cure for T1DB. We propose a gene therapy for T1DM using an adeno-associated viral (AAV) vector that transfects somatic cells with an insulin gene controlled by a glucose-sensitive promoter, thus potentially able to restore glucose-regulated insulin production in diabetic patients. Preliminary testing will be conducted on cell lines to assess the efficiency of the therapy.

With the aim of restoring insulin production in T1DM patients, we propose to genetically modify non-beta cells to produce insulin at high glucose concentration. An engineered AAV vector will deliver the insulin production system into 293 cell lines to be tested. The insulin gene has been cloned and modified to allow for efficient proteolytic cleavage in non-beta cells (furin). In initial tests fluorescent proteins was be used instead of insulin as a reporter, and the effects are assessed using FACS and microscopy.

In short, our project is based on one Gene Therapy Platform and three independently functioning Insulin Production Systems that would provide a care-free cure for Diabetes Type I.

Click on any of the icons below to read more about our "one platform and three systems".

 

Retrieved from "http://2014.igem.org/Team:Tsinghua/Project"