Team:UESTC-China/Modeling1
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.SensorEditingAreaClass p{position:relative;left:0px; width:1140px; font-size:23px; font-family: calibri, arial, helvetica, sans-serif; text-align:justify;} | .SensorEditingAreaClass p{position:relative;left:0px; width:1140px; font-size:23px; font-family: calibri, arial, helvetica, sans-serif; text-align:justify;} | ||
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- | <h1 style="color:#1b1b1b; position:relative; left:0px; padding:15 5px; font-size:35px; font-family: calibri, arial, helvetica, sans-serif; font-weight: bold;font-style: Italic; text-align:center; width:1140px;">Photosynthetic | + | <h1 style="color:#1b1b1b; position:relative; left:0px; padding:15 5px; font-size:35px; font-family: calibri, arial, helvetica, sans-serif; font-weight: bold;font-style: Italic; text-align:center; width:1140px;">Photosynthetic formaldehyde assimilation pathway</h1> |
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- | <h1 class="SectionTitles" style=" width:1100px;">Mathematical | + | <h1 class="SectionTitles" style=" width:1100px;">Mathematical principles</h1> |
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<p style="color:#1b1b1b;">Almost all chemical reactions obey the law of constant proportion:</p> | <p style="color:#1b1b1b;">Almost all chemical reactions obey the law of constant proportion:</p> | ||
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- | <h1 class="SectionTitles" style=" width:1100px;">Photosynthetic | + | <h1 class="SectionTitles" style=" width:1100px;">Photosynthetic formaldehyde assimilation pathway</h1> |
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- | <p style="color:#1b1b1b;">The metabolism of photosynthetic | + | <p style="color:#1b1b1b;">The metabolism of photosynthetic formaldehyde assimilation was shown on Fig.1. Since the substrate (Ru5P) and product (F6P) of the sequential reactions catalyzed by HPS and PHI are intermediates of the Calvin cycle in plants, photosynthesis could provide sufficient substrates for the reactions catalyzed by HPS and PHI if the two enzymes were expressed in plant <i>(Song et al., 2010)</i>. It has been proved that over-expressing the HPS/PHI fusion protein can enhance the ability of the plants to absorb and assimilate exogenous formaldehyde <i>(Chen et al., 2010)</i>. In this case, we utilize the mathematical principles described above to analyze the metabolism.</p> |
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- | <div><img style="width:50% ;" src="https://static.igem.org/mediawiki/2014/ | + | <div><img style="width:50% ;" src="https://static.igem.org/mediawiki/2014/3/3f/Regu1.png"></div> |
<div><p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:1100px; color:#1b1b1b;"> | <div><p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:1100px; color:#1b1b1b;"> | ||
- | <strong>Fig.1</strong> Schematic diagram of photosynthetic | + | <strong>Fig.1</strong> Schematic diagram of photosynthetic formaldehyde assimilation pathway. Ru5P, D-ribulose 5-phosphate; Hu6P, D-arabino-3-hexulose 6-phosphate; F6P, fructose 6-phosphate; Xu5P, xylulose 5-phosphate; RuBP, ribulose 1,5-bisphosphate; 3-PGA, glycerate 3-phosphate; FBP, fructose-1,6-bisphosphatase; |
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- | <p style="color:#1b1b1b;">Simplify the system (Fig.2), consist of the input of | + | <p style="color:#1b1b1b;">Simplify the system (Fig.2), consist of the input of formaldehyde and CO2, the recycle of Ru5P and the output of F6P:</p> |
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<div><img style="width:50% ;" src="https://static.igem.org/mediawiki/2014/4/45/MFig-2.jpg"></div> | <div><img style="width:50% ;" src="https://static.igem.org/mediawiki/2014/4/45/MFig-2.jpg"></div> | ||
- | <div><p style="position:relative; left: | + | <div><p style="position:relative; left:10px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:600px; color:#1b1b1b;"> |
<strong>Fig.2</strong> A simplified version of photosynthetic HCHO assimilation pathway | <strong>Fig.2</strong> A simplified version of photosynthetic HCHO assimilation pathway | ||
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<h1 class="SectionTitles" style="width:1100px;">Results</h1> | <h1 class="SectionTitles" style="width:1100px;">Results</h1> | ||
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- | <p style="color:#1b1b1b;">By changing the value of those parameters (<i>k1, k2</i> … ), we obtained the relationship (Fig.3) between the concentration of different components (Ru5P, F6P and | + | <p style="color:#1b1b1b;">By changing the value of those parameters (<i>k1, k2</i> … ), we obtained the relationship (Fig.3) between the concentration of different components (Ru5P, F6P and formaldehyde) versus the time. </p> |
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- | <div><img style="width: | + | <div><img style="width:60% ;" src="https://static.igem.org/mediawiki/2014/d/d2/Uestc_newModel1.jpg"></div> |
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<strong>Fig.3</strong> The diagram of concentration versus time. A for Ru5P and F6P, B for HCHO and C for those three components | <strong>Fig.3</strong> The diagram of concentration versus time. A for Ru5P and F6P, B for HCHO and C for those three components |
Latest revision as of 01:55, 18 October 2014