Team:CityU HK/collaboration

From 2014.igem.org

(Difference between revisions)
 
(78 intermediate revisions not shown)
Line 4: Line 4:
<style>
<style>
-
#main {background-color: #F0EEEA;}
 
#title {
#title {
Line 10: Line 9:
text-align: center;
text-align: center;
margin-bottom: 40px;
margin-bottom: 40px;
-
font-size: 3em;
+
font-size: 1.7em;
font-family: "brandon-grotesque", sans-serif;
font-family: "brandon-grotesque", sans-serif;
font-weight: 700;
font-weight: 700;
text-shadow: 0px 3px 2px rgba(0, 0, 0, 0.2);
text-shadow: 0px 3px 2px rgba(0, 0, 0, 0.2);
-
padding-top: 4%;
+
padding-top: 0.5%;
letter-spacing: 1px;
letter-spacing: 1px;
}
}
Line 22: Line 21:
       position: relative;
       position: relative;
       top:0;
       top:0;
 +
}
 +
#main h3 {
 +
      margin-left: 10%;
 +
      margin-right: 10%;
 +
      padding-left: 4%;
 +
      padding-right: 5%;
 +
      color: #3cc;
 +
      font-weight:600;
 +
      font-size: 2em;
 +
      border-bottom: solid 2px #ebebeb;
 +
}
 +
#main p {
 +
      padding-left: 17%;
 +
      padding-right: 15%;
}
}
#main #content {
#main #content {
 +
      background-color: #F0EEEA;
       text-align: center;  
       text-align: center;  
-
       padding: 10px 20px 60px 20px;
+
       padding: 40px 20px 30px 20px;
}
}
 +
#main a {color:#3cc;}
 +
#main a:hover {
 +
      color: orange;
 +
      text-decoration: none;}
 +
#main a:visited {color: orange;}
 +
 +
img.displayed {
 +
                      display: block;
 +
                      margin-left: auto;
 +
                      margin-right: auto }
 +
 +
.fig {
 +
    border: none;
 +
    margin-left: 22%;
 +
    margin-right: 22%;
 +
}
 +
 +
.fig th {vertical-align: top;
 +
    width: 12%;
 +
}
 +
 +
.fig td {vertical-align: top;
 +
    text-align: justify;
 +
    font-size: 16px;
 +
    color: #333333;
 +
}
 +
 +
#parameter {margin:0 22%; 0 22%;}
 +
#parameter td, #parameter th {
 +
        border: 2px solid #bdc3c7;
 +
        padding: 2px 3px 2px 3px;
 +
        }
 +
#parameter h2 {font-size: 1.2em; color:#444;}
 +
#parameter p { color: #000;}
 +
 +
.arrow-down {
 +
width: 0;
 +
height: 0;
 +
border-left: 50px solid transparent;
 +
border-right: 50px solid transparent;
 +
border-top: 30px solid #fff;
 +
        position: relative;
 +
        top: 20px;
 +
        margin: 0 auto;
 +
        z-index: 2;
 +
}
 +
#arrow2 {
 +
        border-top: 30px solid #F0EEEA;
 +
        position: relative;
 +
        top: -10px;
 +
}
</style>
</style>
Line 36: Line 101:
<img id="banner"  src="https://static.igem.org/mediawiki/2014/4/4b/CityU_HK_collaboration-banner.png" width=100%>
<img id="banner"  src="https://static.igem.org/mediawiki/2014/4/4b/CityU_HK_collaboration-banner.png" width=100%>
-
<p id="content"> This page is still under construction :) </p>
 
-
</div> <!-- end of #main -->
+
<div id="content">
 +
<h2 id="title">CUHK : Computer simulation of the “Nitrogenase System”  </h2>
 +
<p> This year, the CityU HK iGEM team collaborated with the <a href="https://2014.igem.org/Team:Hong_Kong-CUHK">CUHK iGEM team.</a></p><br>
-
<!--==================== footer ===========================-->
+
<h2 style="color:black"> How did we help the CUHK iGEM team? </h2><br>
-
<div id="footer" class="row">
+
<p>We met the CUHK iGEM team in early October 2014. At the meeting, each party presented our projects and we were impressed with each other’s project goals. Following our discussion, the CityU team agreed to collaborate with the CUHK iGEM team to perform computer simulation/modelling of the “<b><a href="https://2014.igem.org/Team:Hong_Kong-CUHK/home.html?page=link-modelling">Nitrogenase System</a></b>” that the CUHK team was working on. We succeeded in producing a modelling system for their Nitrogenase project.</p><br>
-
<div class="col-md-7">
+
</div>
-
      <h3>Sponsors</h3>
+
-
      <a href="http://www.cityu.edu.hk/" target="_blank"><img src="https://static.igem.org/mediawiki/2014/9/9c/CityU_HK_citylogo.png" width="95"></a>
+
-
    <a href="http://www6.cityu.edu.hk/bhdbapp/deptweb/index.html" target="_blank"><img src="https://static.igem.org/mediawiki/2014/9/98/CityU_HK_bchlogo.png" width="80"></a>
+
-
    <img src="https://static.igem.org/mediawiki/2014/b/b1/CityU_HK_Invitrogen.png" width="100">
+
-
    <img src="https://static.igem.org/mediawiki/2014/7/7a/CityU_HK_Neblogocol.png" width="135">
+
-
    <img src="https://static.igem.org/mediawiki/2014/d/dc/CityU_HK_IDT-Logo.png" width="125">
+
-
</div> <!-- end of col-md-7-->
+
-
<div class="col-md-5">
+
<div class="container"><div class="row"><div class="col-md-12">
-
      <h3>Stay connected!</h3>
+
<div class="arrow-down" id="arrow2"></div>
-
      <p>email: <a href="mailto:cityuhk.igem@gmail.com">cityuhk.igem@gmail.com</a></p>
+
</div></div></div>
-
      <a href="https://www.facebook.com/igem.2014CityU"><img src="https://static.igem.org/mediawiki/2014/3/31/CityU_HK_fb_logo.png" width="50" height="48"></a>
+
-
      <a href="https://twitter.com/CityUHK_iGEM"><img src="http://ibo2014.org/wordpress/wp-content/themes/ibo2014/img/navbar/twitter_icon.png" width="49" height="51"></a>
+
-
      <a href="#"><img src="https://static.igem.org/mediawiki/2014/1/12/CityU_HK_youtube_logo.png" width="52" height="50"></a>
+
-
</div> <!--end of col-md-5-->
+
-
</div>  
+
-
<div class="row">
+
<br>
-
<div id="copyright" class="col-md-12">
+
 
-
<p>Copyright &copy; iGEM CityU HK 2014. All Rights Reserved</p>
+
<h3>The Model</h3>
-
</div>
+
<p> The computer simulation works on two enzyme kinetic systems as shown below.</p>
-
</div>
+
 
-
<!--end of footer-->
+
<br><center><img class="display" src="https://static.igem.org/mediawiki/2014/1/11/CityU_HK_collaboration-fig1.png" width="70%"></center>
 +
<table class="fig">
 +
  <tr>
 +
    <th>Fig. 1 </th>
 +
    <td>Two Enzyme Kinetic Systems. (a) System I-a single one. (b) System II-combination of two kinetic systems.</td>
 +
  </tr>
 +
</table>
 +
<br><br>
 +
 
 +
<p>The table below presents the Vmax and Km of both enzyme systems.</p><br>
 +
 
 +
<center><table id="parameter">
 +
  <tr>
 +
      <td></td>
 +
      <td><h2>Nitrogenase (30 °C)</h2></td>
 +
      <td><h2>Hydrogenase (80 °C)</h2></td>
 +
  </tr>
 +
  <tr>
 +
      <td><h2>Vmax</h2></td>
 +
      <td><p>0.0175 nmol CH4/ nmol MoFe protein /sec</p></td>
 +
      <td><p>5.28x10-6 nmol H+/ nmol hydrogenase/ sec</p></td>
 +
  </tr>
 +
  <tr>
 +
      <td><h2>Km </h2></td>
 +
      <td><p>16 mM HCO3</p></td>
 +
      <td><p>0.037 mM H2</p></td>
 +
  </tr>
 +
</table></center>
 +
<br><br>
 +
 
 +
<p>Based on the parameters provided, we compared the two enzyme kinetic systems using Matlab simulation.</p><br>
 +
 
 +
<h3>Equations</h3>
 +
<center><img  class="display" src="https://static.igem.org/mediawiki/2014/7/7b/CityU_HK_collaboration-eqn.png"width="40%"></center><br><br>
 +
 
 +
<h3>Results</h3>
 +
<p> A comparison of the two systems using Matlab simulation is shown in Fig. 2. The difference between the product output of the two systems are provided by the data in <a href="https://static.igem.org/mediawiki/2014/4/45/CityU_HK_collaboration-fig4.png">Fig.4</a> and <a href="https://static.igem.org/mediawiki/2014/0/09/CityU_HK_collaboration-fig5.png">Fig.5</a>. </p>
 +
 
 +
<br><center><img style="border:solid #ebebeb" class="display" src="https://static.igem.org/mediawiki/2014/3/39/CityU_HK_collaboration-fig2.png"width="50%"></center><br>
 +
<table class="fig">
 +
  <tr>
 +
    <th>Fig. 2 </th>
 +
    <td>Matlab code. Input: V1max, K1m and A10 are the initial parameter settings of the system I. V2max, K2m are those of system II. t represents the time.</td>
 +
  </tr>
 +
</table><br><br>
 +
 
 +
<br><center><img class="display" src="https://static.igem.org/mediawiki/2014/a/a6/CityU_HK_collaboration-fig3.png" width="70%"></center><br>
 +
<table class="fig">
 +
  <tr>
 +
    <th>Fig. 3 </th>
 +
    <td>A. Relationship between substrate [A] and product [C]. B. Relationship between substrate [A] and difference between [C3] and [C1] . </td>
 +
  </tr>
 +
</table>
 +
 
 +
<br><br>
 +
 
 +
<h3>Acknowledgement</h3>
 +
<p> The modelling was performed by <b>Dr. Zhu Yuan</b> of the Department of Biology and Chemistry, City University of Hong Kong. </p>
 +
 
 +
<br><br><br>
 +
 
 +
</div> <!-- end of #main -->
</body>
</body>
</html>
</html>
 +
{{:Team:CityU_HK/Template/footer}}

Latest revision as of 00:23, 18 October 2014

Bootstrap 101 Template

CUHK : Computer simulation of the “Nitrogenase System”

This year, the CityU HK iGEM team collaborated with the CUHK iGEM team.


How did we help the CUHK iGEM team?


We met the CUHK iGEM team in early October 2014. At the meeting, each party presented our projects and we were impressed with each other’s project goals. Following our discussion, the CityU team agreed to collaborate with the CUHK iGEM team to perform computer simulation/modelling of the “Nitrogenase System” that the CUHK team was working on. We succeeded in producing a modelling system for their Nitrogenase project.



The Model

The computer simulation works on two enzyme kinetic systems as shown below.


Fig. 1 Two Enzyme Kinetic Systems. (a) System I-a single one. (b) System II-combination of two kinetic systems.


The table below presents the Vmax and Km of both enzyme systems.


Nitrogenase (30 °C)

Hydrogenase (80 °C)

Vmax

0.0175 nmol CH4/ nmol MoFe protein /sec

5.28x10-6 nmol H+/ nmol hydrogenase/ sec

Km

16 mM HCO3

0.037 mM H2


Based on the parameters provided, we compared the two enzyme kinetic systems using Matlab simulation.


Equations



Results

A comparison of the two systems using Matlab simulation is shown in Fig. 2. The difference between the product output of the two systems are provided by the data in Fig.4 and Fig.5.



Fig. 2 Matlab code. Input: V1max, K1m and A10 are the initial parameter settings of the system I. V2max, K2m are those of system II. t represents the time.




Fig. 3 A. Relationship between substrate [A] and product [C]. B. Relationship between substrate [A] and difference between [C3] and [C1] .


Acknowledgement

The modelling was performed by Dr. Zhu Yuan of the Department of Biology and Chemistry, City University of Hong Kong.




Bootstrap 101 Template

Retrieved from "http://2014.igem.org/Team:CityU_HK/collaboration"