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Team:Caltech/week3
From 2014.igem.org
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| - | <tr><td colspan= | + | <tr> <td bgColor=#FFFFFF colspan = 3 height = 60px> <font size = +5> <center>Notebook </center> </td> </tr> |
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<b><a href='week11'>Week 11</a></b><br><br> | <b><a href='week11'>Week 11</a></b><br><br> | ||
<b><a href='week12'>Week 12</a></b><br><br> | <b><a href='week12'>Week 12</a></b><br><br> | ||
| + | <b><a href='week13'>Week 13</a></b><br><br> | ||
| + | <b><a href='week14'>Week 14</a></b><br><br> | ||
| + | <b><a href='week15'>Week 15</a></b><br><br> | ||
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| - | <ul><li></li> | + | To figure out what went wrong during assembly of the combinatorial promoter constructs used in last week's TXTL workshop, we attempted to take out the LacI and TetR genes present in plasmids pAA001. |
| - | <li></li> | + | <ul><li>PCR extraction of linear fragments of pAA001 minus the lacI and tetR genes.</li> |
| - | <li></li> | + | <li>Recircularization of the truncated "backbone"+"combinatorial promoter construct" round-the-horn ligation</li> |
| - | <li> | + | <li>Transformation of recircularized plasmids into JM109 competent cells</li> |
| - | + | <li>Cells plated onto carbenicillin plates and incubated at 37°C overnight</li> | |
</ul> | </ul> | ||
| + | <br> | ||
| + | In other news, we continued to work on the wiki and make it worse and worse...... | ||
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| - | <ul><li></li> | + | <ul><li>Colony PCR of colonies that grew up last night</li> |
| - | <li></li> | + | <li>Due to the long weekend, we decided that we will wait until next week to begin minipreps</li> |
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Latest revision as of 00:34, 15 September 2014
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