Team:Groningen/testenv

From 2014.igem.org

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</html>{{:Team:Groningen/Template/MODULE/menu|lactoaid}}<html>
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</html>{{:Team:Groningen/Template/MODULE/header|home|d/df/Overlay.home.png}}<html>
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<!-- DON'T EVER USE THIS CODE START -->
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<div class="entry gridcell grid spanall">
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</html>{{:Team:Groningen/Template/MODULE/tmpl_textmodule|Templates (Remove me before deadline)|
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===Copy your base from these templates===
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*[[Team:Groningen/Template/MODULE/tmpl contentmodule|Content Module Template]]
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*[[Team:Groningen/Template/MODULE/tmpl referencemodule|Reference Module Template]]
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*[[Team:Groningen/Template/MODULE/tmpl tablemodule|TableModule Template]] (always one table per page)
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*[[Team:Groningen/Template/MODULE/tmpl page|Page Template]]
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Alwayse make a new page for each of these type templates listed above. '''never nest''' snippets given within these templates. When you dublicate snippets '''always copy from END to START''':
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<pre>
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<-- NAME SNIPPET START -->
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...
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<-- NAME SNIPPET END -->
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</pre>
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rules: '''OPTIONAL''' code can be removed, a '''SNIPPET''' can be duplicated, '''MANDATORY''' must be used and not removed.
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===Use these functions===
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<pre>
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</html>{{:Team:Groningen/Template/MODULE/tmpl_breadcrummodule|tail|head}}<html>
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</html>{{:Team:Groningen/Template/MODULE/tmpl_imagemodule|title|imagefile|text}}<html>
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</html>{{:Team:Groningen/Template/MODULE/tmpl_imagelinkmodule|title|imagefile|link|text}}<html>
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</html>{{:Team:Groningen/Template/MODULE/tmpl_moviemodule|moviemp4}}<html>
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</html>{{:Team:Groningen/Template/MODULE/tmpl_imagelinkonlymodule|imagefile|link}}<html>
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</html>{{:Team:Groningen/Template/MODULE/tmpl_imageonlymodule|imagefile}}<html>
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</html>{{:Team:Groningen/Template/MODULE/tmpl_textmodule|title|text}}<html>
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</html>{{:Team:Groningen/Template/MODULE/tmpl_textlinkmodule|title|link|text}}<html>
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</html>{{:Team:Groningen/Template/MODULE/tmpl_vcardmodule|photofile|fullname|function|department|phone|email|linkedin|description}}<html>
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</pre>
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===Functions for use within contentmodule template===
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<pre>
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</html>{{:Team:Groningen/Template/MODULE/newfigure|name|figurefile|caption}}<html>
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</html>{{:Team:Groningen/Template/MODULE/newfigurevideo|name|figurefile|videofile|caption}}<html>
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</pre>
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===Other pages===
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*[[Team:Groningen/testenv/medialinks|Media Links]] for all images and movies
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for large text do it this way
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<pre>
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</html>{{:Team:Groningen/Template/MODULE/tmpl_textmodule|title|
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text
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<pre></html>{{:Team:Groningen/Template/MODULE/tmpl_textlinkmodule|title
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|link|
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text
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If functions do not worke properly still use them, this simply means it is not yet implemented.
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<div class="entry gridcell grid">
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<!-- left colmn modules here -->
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{{:Team:Groningen/Template/MODULE/tmpl_breadcrummodule|Test Environment| Hello}}
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{{:Team:Groningen/Template/MODULE/tmpl_contentmodule}}
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</html>{{:Team:Groningen/Template/MODULE/tmpl_moviemodule|moviemp4}}<html>
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</html>{{:Team:Groningen/Template/MODULE/Notebook/toolbox/appendix/table1}}<html>
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<div class="entry grid gridcell spanall">
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</html>{{:Team:Groningen/Template/MODULE/Notebook/toolbox/appendix/table1}}<html>
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</div>
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<div class="entry grid gridcell spanall">
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</html>{{:Team:Groningen/Template/MODULE/Notebook/toolbox/appendix/table2}}<html>
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</html>{{:Team:Groningen/Template/MODULE/Notebook/toolbox/appendix/table3}}<html>
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Latest revision as of 02:18, 18 October 2014

 
 
> LactoAid
 
 
Abstract
Background
 
 
> Project
 
 
The Bandage
Detection
Secretion
Toolbox
Parts
Model-Based Design
Characterization
Interlab Measurement Study
 
 
> Team
 
 
Members
Supervisors
Official Team Page
Acknowledgements
Sponsors
 
 
> Notebook
 
 
Secretion
Detection
The Bandage
Toolbox
Characterization
Vector
Protocols
 
 
> Policy & Practice
 
 
Events
Future Perspectives
Martini Hospital
Media Coverage
Collaboration
 
 
> Awards
 
 
Checklist
Prizes
 
 
> iGEM
 
 
 
 
 
 
 
 
 
Templates (Remove me before deadline)
 

Contents

Copy your base from these templates

Alwayse make a new page for each of these type templates listed above. never nest snippets given within these templates. When you dublicate snippets always copy from END to START: 

<-- NAME SNIPPET START -->
...
<-- NAME SNIPPET END -->

rules: OPTIONAL code can be removed, a SNIPPET can be duplicated, MANDATORY must be used and not removed.

Use these functions

</html>{{:Team:Groningen/Template/MODULE/tmpl_breadcrummodule|tail|head}}<html>

</html>{{:Team:Groningen/Template/MODULE/tmpl_imagemodule|title|imagefile|text}}<html>

</html>{{:Team:Groningen/Template/MODULE/tmpl_imagelinkmodule|title|imagefile|link|text}}<html>

</html>{{:Team:Groningen/Template/MODULE/tmpl_moviemodule|moviemp4}}<html>

</html>{{:Team:Groningen/Template/MODULE/tmpl_imagelinkonlymodule|imagefile|link}}<html>

</html>{{:Team:Groningen/Template/MODULE/tmpl_imageonlymodule|imagefile}}<html>

</html>{{:Team:Groningen/Template/MODULE/tmpl_textmodule|title|text}}<html>

</html>{{:Team:Groningen/Template/MODULE/tmpl_textlinkmodule|title|link|text}}<html>

</html>{{:Team:Groningen/Template/MODULE/tmpl_vcardmodule|photofile|fullname|function|department|phone|email|linkedin|description}}<html>

Functions for use within contentmodule template

</html>{{:Team:Groningen/Template/MODULE/newfigure|name|figurefile|caption}}<html>
</html>{{:Team:Groningen/Template/MODULE/newfigurevideo|name|figurefile|videofile|caption}}<html>

Other pages

for large text do it this way 

</html>{{:Team:Groningen/Template/MODULE/tmpl_textmodule|title|
text
}}<html>

with large arguments or to many arguments place argument on new line (with argument seperator) 

</html>{{:Team:Groningen/Template/MODULE/tmpl_textlinkmodule|title
|link|
text
}}<html>

If functions do not worke properly still use them, this simply means it is not yet implemented.

 
 
 
Test Environment > Hello
 
 
 
 
 
 
 
 
image1
 
 
 
 
 
Primers for making BioBricks out of the nisin operon
 
Primer Sequence
NisA forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA
NisA reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA
NisB forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAAAAGTTCATTTAA
NisB reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCATGTATTCTT
NisT forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA
NisT reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT
NisC forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATAAAAAAAATATAAA
NisC reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCCTCTTCCCTC
NisI forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGAAGATATTTAATACT
NisI reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTACTAATTTCCTACCTTCG
NisP forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAAAAAATACTAGGTTT
NisP reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC
NisRK forward GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGTATAAAATTTTAATAGT
NisRK reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTTTTTATTTTTA
NisFEG forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA
NisFEG reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT
PNisI+RBS forward GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCACTGGATAATGACTATT
PNisI+RBS reverse TACTAGTAGCGGCCGCTGCAGGAAGAAACTTCCTCTTCCCTCCTTTCAA
 
 
 
 
fullname
description
Function:
function
 
 
 
 
 
 
 
title
 
 
title
 
text
 
 
 
 
title
 
text
 
 
 
Primers for making BioBricks out of the nisin operon
 
Primer Sequence
NisA forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA
NisA reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA
NisB forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAAAAGTTCATTTAA
NisB reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCATGTATTCTT
NisT forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA
NisT reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT
NisC forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATAAAAAAAATATAAA
NisC reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCCTCTTCCCTC
NisI forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGAAGATATTTAATACT
NisI reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTACTAATTTCCTACCTTCG
NisP forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAAAAAATACTAGGTTT
NisP reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC
NisRK forward GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGTATAAAATTTTAATAGT
NisRK reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTTTTTATTTTTA
NisFEG forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA
NisFEG reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT
PNisI+RBS forward GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCACTGGATAATGACTATT
PNisI+RBS reverse TACTAGTAGCGGCCGCTGCAGGAAGAAACTTCCTCTTCCCTCCTTTCAA
 
 
 
 
Primers for making the new sfGFP(Bs) BioBrick
 
Primer Sequence
sfGFP(Bs) forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTCAAAAGGAGAAGAGCT
sfGFP(Bs) reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTATAAAGCTCAT
 
 
 
 
Primers for making BioBricks out of genes with illegal restriction sites
 
The primers are denoted with the position of the illegal restriction site that they should remove. Illegal EcoRI sites are abbreviated with an E, XbaI sites with an X, SpeI site with an S and PstI sites with a P.
 
Gene Primer Sequence
NisT Prefix forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA
  S980 reverse TTGTTCCATAAACAAACTTGTATTATAAATGATGTAAATA
  S980 forward TATTTACATCATTTATAATACAAGTTTGTTTATGGAACAA
  S1210 reverse CCATAGTTGGTTGATATAATCCTGAAATTATCTTTACTTGTGTAC
  S1210 forward GTACACAAGTAAAGATAATTTCAGGATTATATCAACCAACTATGG
  Suffix reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT
  Suffix forward ATGAATAATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA
  Prefix reverse CACTTCATCCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG
NisP Prefix forward GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGAAAAAAATACTAGGTTT
  P225 reverse AGTCCTTGTTGTCGATTTTACTGCTGGTGACTGCGCCCCT
  P225 forward AGGGGCGCAGTCACCAGCAGTAAAATCGACAACAAGGACT
  E347 reverse AACGCTATCTCTCTTACTAAATTCAGAACTAACTTGAGTT
  E347 forward AACTCAAGTTAGTTCTGAATTTAGTAAGAGAGATAGCGTT
  E1657 reverse CAATACTCTATTCCCATTAACTTCTGGACTATTCATTAGA
  E1657 forward TCTAATGAATAGTCCAGAAGTTAATGGGAATAGAGTATTG
  Suffix reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC
  Suffix forward AAATTGATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA
  Prefix reverse CCTAGTATTTTTTTCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG
NisFEG Prefix forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA
  P186 reverse TCCAGTATCAGCAGAAATTAAACCAAACAAAATTTTCATC
  P186 forward GATGAAAATTTTGTTTGGTTTAATTTCTGCTGATACTGGA
  E336/X349 reverse CCTGTTTCTGCCAAACCAATCACTTCTAGTGTTTCATGTATTCTCTTA
  E336/X349 forward TAAGAGAATACATGAAACACTAGAAGTGATTGGTTTGGCAGAAACAGG
  Suffix reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT
  Suffix forward TAGATAATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA
  Prefix reverse TTTTACCTGCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG
 
 
 
 
 
 
© 2014 iGem Groningen, Design: Randy Wind; Art: Andries de Vries, Freek de Wijs and Bente Lauxen; Photography: Bente Lauxen; Coding: Randy Wind and Anco Gietema.
 
 
 
 
 

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