Team:AHUT China/Notebook

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<br>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:AHUT_China/Notebook&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:AHUT_China"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:AHUT_China/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=AHUT_China"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:AHUT_China/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:AHUT_China/Parts"style="color:#000000"> Parts</a></td>
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<a href="https://2014.igem.org/Team:AHUT_China/Modeling"style="color:#000000"> Modeling</a></td>
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<a href="https://2014.igem.org/Team:AHUT_China/Notebook"style="color:#000000"> Notebook</a></td>
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<a href="https://2014.igem.org/Team:AHUT_China/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:AHUT_China/Attributions"style="color:#000000"> Attributions </a></td>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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<tr><td colspan="3"> <h3>Notebook</h3></td></tr>
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<td width="45%"  valign="top">
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<p>You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well. </p>
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  <div id="block_pic_1">
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      <p style="width:800px; height:100px; padding:100px 80px; text-align:center; margin:0 auto; color:#f8f8f8; font-size:48px; font-family:'Trebuchet MS', Helvetica, sans-serif;">A man can fail many times, but he isn't a failure until he begins to blame somebody else. </p>
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<p style="height:100px; width:960px; line-height:100px; margin: 0 auto; text-align:right; color:#ffffff;font-size:24px; font-family:'Trebuchet MS', Helvetica, sans-serif;">
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---J. Burroughs. Averican naturalist
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</p>
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    </div>
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    <div style="background-color:#ffffff; padding-bottom:30px;">
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      <div class="topic">
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      <a name="n">NOTEBOOK</a>
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      </div>
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      <div class="triangle"><img src="https://static.igem.org/mediawiki/2014/2/22/AHUT_CHINA_2014_triangle.png" width="143" height="35" /></div>
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      <div class="block_content" style="text-align:center">
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        <img src="https://static.igem.org/mediawiki/2014/8/85/AHUT_CHINA_2014_notebook_pic_1.png" width="600" height="1900" />
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      </div><!--block_content-->
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    </div><!--color-->
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    <div style="background-color:#ffffff; padding-bottom:30px;">
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      <div class="topic">
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      <a name="p">PROTOCOLS</a>
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      </div>
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      <div class="triangle"><img src="https://static.igem.org/mediawiki/2014/2/22/AHUT_CHINA_2014_triangle.png" width="143" height="35" /></div>
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      <div class="block_content">
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        <p class="content_topic" style="">Taq Plus PCR</p>
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        <p style="width:300px; padding:0 100px; margin-top:20px; color:#414141;" class="content_word f_left">
 +
MasterMix<br />
 +
0.1 U Taq Plus Polymerase/μl<br />
 +
500 μM dNTP each<br />
 +
20 mM Tris-HCl (pH8.3)<br />
 +
100 mM KCl<br />
 +
3 mM MgCl2<br />
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<img src="https://static.igem.org/mediawiki/2014/a/ae/AHUT_CHINA_2014_notebook_pic_2.jpg" width="192" height="89" />
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</p>
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        <div class="hp_pic f_left" style="width:400px; margin:0px; text-align:center;">
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          <a><img src="https://static.igem.org/mediawiki/2014/b/b0/AHUT_CHINA_2014_notebook_pic_3.jpg" width="222" height="267" /></a>
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        </div>
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        <p class="content_topic c_b" style="padding-top:20px;">TIANgel Midi Purification Kit</p>
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        <div style="text-align:center;"><img src="https://static.igem.org/mediawiki/2014/3/31/AHUT_CHINA_2014_notebook_pic_4.jpg" width="328" height="131" /></div>
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        <p class="content_topic c_b" style="padding-top:20px;">DNA MW Standard Marker</p>
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        <div style="text-align:center;"><img src="https://static.igem.org/mediawiki/2014/1/16/AHUT_CHINA_2014_notebook_pic_5.png" width="474" height="188" /></div>
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        <p class="content_topic c_b" style="padding-top:20px;">LB media</p>
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        <table>
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          <tr>
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            <td>1L LB medium</td>
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            <td>1%</td>
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          </tr>
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          <tr>
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            <td>Yeast Extract</td>
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            <td>0.5%</td>
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          </tr>
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          <tr>
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            <td>aCl</td>
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            <td>1%</td>
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          </tr>
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        </table>
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        <p class="content_topic c_b" style="padding-top:20px;">E.coli</p>
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        <p style="text-align:center;">DH5a<br />Top10</p>
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        <p class="content_topic c_b" style="padding-top:20px;">Plasmids</p>
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        <div style="text-align:center;"><img src="https://static.igem.org/mediawiki/2014/f/f4/AHUT_CHINA_2014_notebook_pic_6.jpg" width="451" height="384" /></div>
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        <p>pUC57:http://www.ncbi.nlm.nih.gov/nuccore/Y14837.1</p>
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        <p style="text-align:center;">PSB1C3</p>
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        <p class="content_topic c_b" style="padding-top:20px;">DNA Ligation Kit</p>
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        <div style="text-align:center;"><img src="https://static.igem.org/mediawiki/2014/2/26/AHUT_CHINA_2014_notebook_pic_7.jpg" width="470" height="63" /></div>
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        <p class="content_topic c_b" style="padding-top:20px;">Enzyme Cutting</p>
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        <div style="text-align:center;"><img src="https://static.igem.org/mediawiki/2014/5/50/AHUT_CHINA_2014_notebook_pic_8.jpg" width="389" height="317" /></div>
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      <div class="c_b"></div>
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    <div style="background-color:#ffffff; padding-bottom:30px;">
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      <div class="topic">
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      <a name="l">Lab Pictures</a>
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      </div>
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      <div class="triangle"><img src="https://static.igem.org/mediawiki/2014/2/22/AHUT_CHINA_2014_triangle.png" width="143" height="35" /></div>
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      <div class="block_content">
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        <div style="text-align:center; margin-top:20px;" class="hp_pic">
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          <img src="https://static.igem.org/mediawiki/2014/6/6a/AHUT_CHINA_2014_notebook_pic_9.jpg" width="400" height="300" style="border:#f59d34 4px dashed;" />
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          <img src="https://static.igem.org/mediawiki/2014/2/2b/AHUT_CHINA_2014_notebook_pic_10.jpg" width="400" height="300" style="border:#f59d34 4px dashed;" />
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        </div>
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        <div style="text-align:center; margin-top:20px;" class="hp_pic">
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          <img src="https://static.igem.org/mediawiki/2014/d/d1/AHUT_CHINA_2014_notebook_pic_11.jpg" width="400" height="300" style="border:#f59d34 4px dashed;" />
 +
          <img src="https://static.igem.org/mediawiki/2014/8/86/AHUT_CHINA_2014_notebook_pic_15.jpg" width="249" height="300" style="border:#f59d34 4px dashed;" />
 +
        </div>
 +
        <div style="text-align:center; margin-top:20px;" class="hp_pic">
 +
          <img src="https://static.igem.org/mediawiki/2014/4/47/AHUT_CHINA_2014_notebook_pic_12.jpg" width="400" height="225" style="border:#f59d34 4px dashed;" />
 +
          <img src="https://static.igem.org/mediawiki/2014/6/6e/AHUT_CHINA_2014_notebook_pic_13.jpg" width="400" height="225" style="border:#f59d34 4px dashed;" />
 +
        </div>
 +
        <div style="text-align:center; margin-top:20px;">
 +
          <img src="https://static.igem.org/mediawiki/2014/0/07/AHUT_CHINA_2014_notebook_pic_14.jpg" width="400" height="225" style="border:#f59d34 4px dashed;" />
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        </div>
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</table>
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    <div style="width:100%; background:url(https://static.igem.org/mediawiki/2014/d/de/AHUT_CHINA_2014_car.png) repeat-x;background-color:#ffffff;  display:block; height:120px;"></div>
 +
   
 +
    <ul id="left_bar">
 +
      <li class="navi1"><a href="http://www.happyvalleychinesefood.com/igem/download/AHUT4iGEM_android.apk"></a></li>
 +
      <li class="navi2"><a href="http://www.happyvalleychinesefood.com/igem/download/AHUT4iGEM_iosbrk.ipa"></a></li>
 +
      <li class="navi3" id="goTopBtn"><a></a></li>
 +
      <script type=text/javascript>goTopEx();</script>
 +
  </ul>
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</div><!--sec_main-->
</html>
</html>
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{{:Team:AHUT_China/footer}}

Latest revision as of 04:09, 17 October 2014

A man can fail many times, but he isn't a failure until he begins to blame somebody else.

---J. Burroughs. Averican naturalist

NOTEBOOK
PROTOCOLS

Taq Plus PCR

MasterMix
0.1 U Taq Plus Polymerase/μl
500 μM dNTP each
20 mM Tris-HCl (pH8.3)
100 mM KCl
3 mM MgCl2

TIANgel Midi Purification Kit

DNA MW Standard Marker

LB media

1L LB medium 1%
Yeast Extract 0.5%
aCl 1%

E.coli

DH5a
Top10

Plasmids

pUC57:http://www.ncbi.nlm.nih.gov/nuccore/Y14837.1

PSB1C3

DNA Ligation Kit

Enzyme Cutting

Lab Pictures

Retrieved from "http://2014.igem.org/Team:AHUT_China/Notebook"